Gene Information

Name : Bcell_3224 (Bcell_3224)
Accession : YP_004096197.1
Strain : Bacillus cellulosilyticus DSM 2522
Genome accession: NC_014829
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : -
Position : 3435582 - 3437336 bp
Length : 1755 bp
Strand : -
Note : KEGG: pyruvate kinase; TIGRFAM: pyruvate kinase; PFAM: pyruvate kinase barrel; pyruvate kinase alpha/beta; PEP-utilising protein mobile region

DNA sequence :
GTGAGAAAAACAAAAATAGTTTGTACGATTGGTCCTGCGAGTGAAACTGTAGAAAAACTAGAACAATTAATATCTGCTGG
AATGAATGTTGCGAGATTAAATTTTTCCCATGGTGATTTTGATGAGCATGGAGCTCGTATTAAAAATATTCGTGAAGCTG
CAAATAAACTAGGTAAACCTGTTGCGATTCTTTTAGATACAAAAGGTCCAGAAATCCGTACACAAACGGTTGAAGGTGGA
GCTATCGAATTAGTGAAAGGCTCTGCGCTTAAAGTAGCTATGACTGAGGTAGTTGGTACGACTGAGAAAATTTCAGTAAC
ATACCCTGGACTTGTTGATGATGTACAAGTTGGTTCAACAATCCTTCTAGATGACGGGTTAATTGAATTGAAGGTTACAG
AAGTCGGTAATGAAGAGTTAACGACTGAAGTAATGAATAGTGGTACATTGAAAAATAAAAAAGGTGTCAACGTTCCTGGT
GTCAGTGTTCAGTTGCCTGGAATTACAGAAAAGGATGCAAACGACATCGTTTTCGGTATTGAGCAAGATGTAGATTTTAT
TGCGCCATCTTTTGTGAGAAAAGCTTCGGATGTGTTAGAACTTCGTGAATTACTTGAAAAGCATAACGCTGGTCATATTC
AAATCATCCCTAAAATTGAAAACCAAGAGGGTGTAGACAATATTGAAGAGATTCTTGAAATCTCTGATGGTTTAATGGTA
GCACGTGGAGACCTTGGCGTTGAAATTCCTGCTGAAGATGTACCGTTAGTTCAAAAAGAGTTAATTAAAAAGTGTAATGC
ACAAGGGAAGCCTGTTATTACAGCTACACAAATGTTAGATTCCATGCAACGTAATCCTCGCCCAACTCGTGCAGAAGCTA
GTGACGTAGCAAACGCTATATTTGATGGTACGGATGCAATTATGCTATCAGGTGAAACGGCTGCCGGTGATTACCCAGTA
GAATCTGTACAAACGATGAGAAACATTGCGTTGAAAACTGAAACAGCGCTTAAATATAAAGATATTCTTCGTAAAAAGAC
GAAGGAAAGTGAACACACTATTACAGATGCAATTAGCCAATCTGTATCACATACTGCTCTTAATTTAAATGCATCAGCTA
TTATTACTGCAACAGAAAGTGGACACACTGCAAAAATGATTGCGAAATATCGTCCGCAATCTCCAATTGCTGCAGTAACA
AGCAGTGAGCGTGTATGTCGATCATTAGCGTTAACTTGGGGTGTATATCCATCTGTAGGTCATAGTGCTGATTCTACTGA
TGAAATGCTACAAATTGCAGTAGAAGGAGCTTTACAGACGGGGTTAGTACAGTATGGTGACCTAGTTGTTATTACTGCAG
GTGTACCAATTAACGAAAAAGGTACAACAAACATTATGAAGGTACATGTAGTAGGTGAAGTTGCTGCTAAAGGGCAAGGT
GTAGGTCGTAAATCTGCTACAGGTAAAGTAGTTGTTGCTAACAGCGCAACAGAAGCTCTTAACAAATTAAATGATGGTGA
TGTATTAGTAACAACAAATACAGACCGTGACATGATGGAGGCTTTTGAAAAAGCTGGAGCAGTTATTACTGAGCAAGGTG
GCCTCACTTCTCATGCTGCTGTTGTTGGACTAAGCTTAGATATTCCTGTAATTGTTGGTGTTGAAAATGTAACTTCACTC
TTTAATGAAGGTGAAGAAGTTACAGTTGACGCGCAACACGGATATGTTTATAAAGGACAAGCAAACGTACTTTAA

Protein sequence :
MRKTKIVCTIGPASETVEKLEQLISAGMNVARLNFSHGDFDEHGARIKNIREAANKLGKPVAILLDTKGPEIRTQTVEGG
AIELVKGSALKVAMTEVVGTTEKISVTYPGLVDDVQVGSTILLDDGLIELKVTEVGNEELTTEVMNSGTLKNKKGVNVPG
VSVQLPGITEKDANDIVFGIEQDVDFIAPSFVRKASDVLELRELLEKHNAGHIQIIPKIENQEGVDNIEEILEISDGLMV
ARGDLGVEIPAEDVPLVQKELIKKCNAQGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGDYPV
ESVQTMRNIALKTETALKYKDILRKKTKESEHTITDAISQSVSHTALNLNASAIITATESGHTAKMIAKYRPQSPIAAVT
SSERVCRSLALTWGVYPSVGHSADSTDEMLQIAVEGALQTGLVQYGDLVVITAGVPINEKGTTNIMKVHVVGEVAAKGQG
VGRKSATGKVVVANSATEALNKLNDGDVLVTTNTDRDMMEAFEKAGAVITEQGGLTSHAAVVGLSLDIPVIVGVENVTSL
FNEGEEVTVDAQHGYVYKGQANVL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 2e-110 52
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 1e-109 52
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 2e-110 52
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 2e-110 52

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Bcell_3224 YP_004096197.1 pyruvate kinase VFG0479 Protein 8e-111 52