Gene Information

Name : Bcell_3089 (Bcell_3089)
Accession : YP_004096063.1
Strain : Bacillus cellulosilyticus DSM 2522
Genome accession: NC_014829
Putative virulence/resistance : Resistance
Product : mercuric reductase
Function : -
COG functional category : C : Energy production and conversion
COG ID : COG1249
EC number : -
Position : 3281620 - 3283260 bp
Length : 1641 bp
Strand : -
Note : KEGG: bpf:BpOF4_20564 mercuric reductase; TIGRFAM: mercuric reductase; PFAM: FAD-dependent pyridine nucleotide-disulphide oxidoreductase; Heavy metal transport/detoxification protein; pyridine nucleotide-disulphide oxidoreductase dimerisation region

DNA sequence :
ATGAAAAAATTTAAATTGTACATTCAAGGAATGACTTGCACAGGGTGTGAAGAACATGTGGGCGAAGCACTTGAAAAAAT
AGGTGCTGAAAATATTGAGACCAGTTTTCATCATGGTGAAGCAGTATTTGAATTGCCAGATGATATTGGTGTTGAAAGTG
CACTAAAGGCAATTCATGAAGCAAAATATCAGCCTGGAGAAATAGAAAGGGTATCATCAGAAGAAAAAGTGGTCTTAGGT
AATGAAGGAGATTATGACCTGCTTATCATTGGTTCTGGTGGTGCTGCTTTTTCGGCTGCAATTAAAGCGATTGAGTATGG
GGCAAAAGTTGCCATGATAGAGCGTGGAACAGTTGGGGGAACATGCGTAAATATTGGTTGTGTTCCATCAAAAACACTCC
TTAGAGCTGGGGAAATCAATCATTTAGCAAAAAATAATTCATTTACAGGTTTACAGACAACTGCTGGAGAAGTGGAATTA
GCACCTTTAATCAAGCAAAAGAATGAATTGGTGAGTGAGCTTCGAAATCAAAAATATGTTGATCTAATTGATGAATATGG
ATTTGATTTAATTGAAGGTGATGCGAAATTTATTGATGAAAATACGGTTAAAGTCAATGGAAAGAAATTTTCTGCAAAGC
GCTTTTTAATTGCAACGGGGGCTTCTCCATCTTTACCCCTGATTTCGGGACTTAAAGAAGTGGAGTATTTAACAAGTACA
ACGCTACTTGAATTAAAGAAAGTACCAAAACGATTAACAGTGATTGGTTCAGGATACATTGGAATGGAGCTTGGACAACT
ATTTCATAATTTAGGTTCAAAAGTAACACTTATGCAAAGGAGTGAGCGACTTTTAAAAGAGTATGATCCAGAGATTTCGG
AAACAGTAGAAAAAGCCTTAGTTGAACAAGGAATAAACCTTGTAAAAGGAGCATCTTTTGAACGTGTTGAACAAGCTGGA
GAAATCAAAAAGGTACATGTAACAGTAGATGGTAAGAAAAAAGTCATTGAATCAGAGCAGTTGCTTGTTGCAACAGGAAG
AAAACCGAATACGGAAAAATTAAATTTACATGTTGCAGGTGTTGAAGTTGGACCTCGAAAAGAAATAAAAATCAATGACT
ATGCGCAAACAAGTAATGAAAAAATTTATGCAGCAGGAGATGTAACACTAGGACCCCAATTTGTATATGTAGCAGCATAT
GAAGGTGGAATTGTTGCTGATAATGCGATTGGTGGGTTAAATAAAAAAATAGATTTATCTGTTGTTCCAGGTGTCACATT
TACAAAACCTTCGATTGCAACGGTTGGATTAACAGAAGAACAAGCAAAAGAAAAAGGCTATGAGGTAAAAACATCTGTCT
TGCCTTTAAGTGCTATTCCAAGAGCGATAGTAAACCGTGAAACAACAGGTGTTTTCAAGCTTGTTGCTGACGCGAAAACA
CTAAAAGTGCTTGGGGTTCATATCGTGTCTGAAAATGCAGGAGATGTGATTTATTCAGCGACATTAGCCGTTAAGTTTGG
CTTAACGGTAGAGGATCTAAAAGAAACTATAGTACCATACTTAACAATGACAGAAGGACTAAAACTAGCTGCTCTTACCT
TCGATAAAGATGTATCAAAATTATCTTGTTGTGCAGGTTAA

Protein sequence :
MKKFKLYIQGMTCTGCEEHVGEALEKIGAENIETSFHHGEAVFELPDDIGVESALKAIHEAKYQPGEIERVSSEEKVVLG
NEGDYDLLIIGSGGAAFSAAIKAIEYGAKVAMIERGTVGGTCVNIGCVPSKTLLRAGEINHLAKNNSFTGLQTTAGEVEL
APLIKQKNELVSELRNQKYVDLIDEYGFDLIEGDAKFIDENTVKVNGKKFSAKRFLIATGASPSLPLISGLKEVEYLTST
TLLELKKVPKRLTVIGSGYIGMELGQLFHNLGSKVTLMQRSERLLKEYDPEISETVEKALVEQGINLVKGASFERVEQAG
EIKKVHVTVDGKKKVIESEQLLVATGRKPNTEKLNLHVAGVEVGPRKEIKINDYAQTSNEKIYAAGDVTLGPQFVYVAAY
EGGIVADNAIGGLNKKIDLSVVPGVTFTKPSIATVGLTEEQAKEKGYEVKTSVLPLSAIPRAIVNRETTGVFKLVADAKT
LKVLGVHIVSENAGDVIYSATLAVKFGLTVEDLKETIVPYLTMTEGLKLAALTFDKDVSKLSCCAG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
SE0085 NP_763640.1 dihydrolipoamide dehydrogenase Not tested SCCpbp4 Protein 0.0 69
merA BAB47642.1 mercuric reductase Not tested Type-III SCCmec Protein 0.0 69
merA AAN62178.1 mercuric (Hg(II)) reductase Not tested PAGI-2(C) Protein 2e-85 42
merA ABQ57371.1 MerA Not tested SGI1 Protein 4e-85 41
merA AGK07021.1 MerA Not tested SGI1 Protein 4e-85 41
merA ACN81005.1 MerA Hg(II) mercuric ion reductase Not tested AbaR5 Protein 2e-84 41
merA AGK07079.1 MerA Not tested SGI1 Protein 4e-85 41
unnamed ABR13401.1 mercuric reductase Not tested PAGI-5 Protein 6e-83 41
merA AET25397.1 MerA Not tested PAGI-2(C) Protein 2e-83 41
merA AFG30120.1 MerA Not tested PAGI-2 Protein 2e-83 41
merA YP_006098387.1 mercuric ion reductase Not tested Tn2411 Protein 3e-83 41
merA CAJ77060.1 Mercuric ion reductase Not tested AbaR1 Protein 2e-84 41

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Bcell_3089 YP_004096063.1 mercuric reductase BAC0224 Protein 0.0 75
Bcell_3089 YP_004096063.1 mercuric reductase BAC0651 Protein 0.0 69
Bcell_3089 YP_004096063.1 mercuric reductase BAC0652 Protein 5e-83 41