Gene Information

Name : Ethha_0761 (Ethha_0761)
Accession : YP_004091061.1
Strain : Ethanoligenens harbinense YUAN-3
Genome accession: NC_014828
Putative virulence/resistance : Resistance
Product : D-alanine--D-alanine ligase
Function : -
COG functional category : M : Cell wall/membrane/envelope biogenesis
COG ID : COG1181
EC number : 6.3.2.4
Position : 832723 - 833787 bp
Length : 1065 bp
Strand : +
Note : TIGRFAM: D-alanine/D-alanine ligase; KEGG: cce:Ccel_0283 D-alanyl-alanine synthetase A; PFAM: D-alanine--D-alanine ligase domain protein

DNA sequence :
TTGAAAAAACCGCGTGTAGCCGTCCTGTTTGGCGGCATGTCCTCGGAGCATGCAGTTTCTCTTGTTTCGGCCACTTCGGT
CATTGCCAATATTCCGCGTGATCGGTTTGATGTAGTAATGATCGGCATCACGCGCGACGGCGAATGGCGGCTGTTTGAAG
GCGATGTTGAAAAACTGGCCGGTTCCGGCTGGGAAAGCGACCCGTCCAACCGGCCGGCGTTCATCGCGCCCGATTCGCGC
GTGCATGGGATCGTGGCGCTTTCACCGGATGGTCGGATCGAAACCATCCGTCTGGACGTCGTGTTCCCGGTGCTCCATGG
TCTGAACGGCGAGGACGGCACGGTGCAGGGGCTGCTGACCCTGGCGCGCATTCCGTTTGTCGGCTGCGGTGTAACGGCTT
CCGCAGTCTGTATGGACAAAGCGATCACCAAAGCGCTGTGCGATACGTACCAGATCCCGCAGGCCAAATGGGAAACGGTG
CGGGTCTCCGATCTGAAAGACAACGGCAAAGCGGTTGTGGAACGCCTGGAAGCGCATCTGGGCTACCCGTTGTTTGTAAA
ACCCGCGAATGCGGGCTCTTCGGTGGGGATTAGCAAAGCGGCCGACCGTGAAACACTCTACAAGGCCATCGAAACCGCTG
CGACGCATGACCGTAAGCTGGTGTTTGAAGAGGCCGTGAACGGCAGAGAAATTGAGTGCGCCGTGCTGGGCAATGAGGAA
CCGGTCGTGTCCTGCTGCGGAGAAATCAAACCCTGCAACGAATTTTATGATTATAACGCCAAATATCTGGCCGGCCGCAC
GGAACTGGTTATCCCGGCGAGTCTGCCGAAAGATGTATCCGAACGCGTACGTCAGACCGCAATGCGCGTGTTTCGGCTTT
TGGGCTGCCGGGGTCTGACGCGCATGGACTTCTTTGCCTTGGAAGACGGGACGGTGCTCCTCAACGAGCCGAATACCATC
CCCGGGTTTACGTCCATCAGCATGTACCCCAAACTGTTCGAAGCCGGTGGCATCTCTTATGGCGAACTGTTGGAAACCCT
GCTGCGGCTGGCGATGGAAGGGTGA

Protein sequence :
MKKPRVAVLFGGMSSEHAVSLVSATSVIANIPRDRFDVVMIGITRDGEWRLFEGDVEKLAGSGWESDPSNRPAFIAPDSR
VHGIVALSPDGRIETIRLDVVFPVLHGLNGEDGTVQGLLTLARIPFVGCGVTASAVCMDKAITKALCDTYQIPQAKWETV
RVSDLKDNGKAVVERLEAHLGYPLFVKPANAGSSVGISKAADRETLYKAIETAATHDRKLVFEEAVNGREIECAVLGNEE
PVVSCCGEIKPCNEFYDYNAKYLAGRTELVIPASLPKDVSERVRQTAMRVFRLLGCRGLTRMDFFALEDGTVLLNEPNTI
PGFTSISMYPKLFEAGGISYGELLETLLRLAMEG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
vanB NP_815951.1 D-alanine--D-lactate ligase Not tested Not named Protein 2e-59 41
vanB YP_008394259.1 Vancomycin B-type resistance protein vanB, D-alanine--D-lactate ligase Not tested Not named Protein 1e-59 41

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Ethha_0761 YP_004091061.1 D-alanine--D-alanine ligase U35369.1.gene6.p01 Protein 8e-60 41
Ethha_0761 YP_004091061.1 D-alanine--D-alanine ligase AE016830.1.gene2250. Protein 8e-60 41
Ethha_0761 YP_004091061.1 D-alanine--D-alanine ligase AF277571.gene.p01 Protein 1e-57 41
Ethha_0761 YP_004091061.1 D-alanine--D-alanine ligase U00456.gene.p01 Protein 8e-60 41
Ethha_0761 YP_004091061.1 D-alanine--D-alanine ligase AY489045.gene.p01 Protein 4e-57 41
Ethha_0761 YP_004091061.1 D-alanine--D-alanine ligase AF310956.2.orf5.gene Protein 6e-60 41
Ethha_0761 YP_004091061.1 D-alanine--D-alanine ligase AY145441.gene.p01 Protein 2e-60 41