Gene Information

Name : ML5_4212 (ML5_4212)
Accession : YP_004083866.1
Strain : Micromonospora sp. L5
Genome accession: NC_014815
Putative virulence/resistance : Resistance
Product : mercuric reductase
Function : -
COG functional category : C : Energy production and conversion
COG ID : COG1249
EC number : -
Position : 4463494 - 4464903 bp
Length : 1410 bp
Strand : -
Note : KEGG: mmi:MMAR_p12 mercuric reductase, MerA; TIGRFAM: mercuric reductase; PFAM: FAD-dependent pyridine nucleotide-disulphide oxidoreductase; pyridine nucleotide-disulphide oxidoreductase dimerisation region

DNA sequence : 
ATGCGGTACGACCTGGCAATCGTCGGCTCCGGTGGCGGTGCCTTCGCGGCGGCGATCGCCGCGCGGCGTAAGGGCAAGCA
CGTGGTGATGGTGGAGCACGGCGACGTGGGCGGCACGTGTGTGAACACCGGGTGCGTACCGTCCAAGGCGTTGCTGGCCG
CCGCGCACGCCCGCCACGTCGCGCTGGACGCGGCCCGCTTCCCGGGAATTCGCGCGAGCGCCGAGCAGGTGGATTTCCAG
GAGCTGATCGCCGGCAAGCGGCGACTGGTGGCAGACATGCGGGCGGACAAGTACGTCGACCTCGCCGCCGAGTACGGCTG
GGAGATCCTCACCGGCCACGGCCGGTTCACCGAGGGCCCGCTGCTGTGCGTCGATCTGCCCGACGGCGGCAGTCAACGCA
TCGAGGCGGAGAACTACCTGATCGCCACCGGCTCGGCCCCGTGGATACCACCCATCGACGGCCTCGCCCAGGTCGGCTAC
CTAACCTCCACCACGGCGATGGAATCCGACCACCTGCCGCAGTCCCTGCTGGTGGTCGGCGGCAACTACATCGGCCTCGA
ACAGGGGCAACTGTTCGCCCGACTCGGCACCCAGGTCACCGTGGTCGAGACCCTCGACCGACTCGCGCCCAAGGAAGAAC
CGGAGGTGTCCGCTGCCATCGAGCAGGTCTTCGCCGACGACGGCATCGCCGTCCACACCACCGCGACCGTACGGTCGGTA
CGCCGCGAGAACGGACACGTCGTCGCCGTGCTCGACGGACAACCCGGCCGACAGATCCACGCCGAGCAGATCCTGATCGC
CACCGGACGCCGCCCAGTTGTCGACGGCCTGAACCTGGAGACGGTCGGAGTCAAGACCGGCCAGCACGGCGAGATCCTGG
TCGACGAGCACCTGCGCACCACCAACCCGCGTATCTGGGCCGCCGGAGACGTCACCGGCCACCCCCAGTTCGTGTACGTC
GCCGGCGCACACGGGTCGATCGTGGCCGACAACGCCTTCGACAACGCGGGTCGCACCGTCGACTACCACCACCTGCCGCA
AGTCACCTTCACCACCCCGGCGATCGCGTCGGCCGGGATGACCGAGGCCCAGGCGATCAGCGCTGGTCACCGCTGCGACT
GCCGGGTACTGCCGCTGGAGTACGTGCCACGCGCGATCGTCAACCGCGACACCCGCGGCCTCATCAAGCTGGTCGCCGAC
GCCGACACCGGCCGACTCCTCGGCGCCCACGTGGTCGCCGACGCCGCCGGAGAGGTCATCGCCACCGCCGTGTACGCCCT
CGCCAACGACATGACCGTGCAGCAGATGGCCGACCTGTGGTGCCCATACCTGACCATGGCCGAAGGCCTCAAACTCGCCG
CCCAGACCTACACCCGCGACGTGTCGAAACTGTCCTGCTGCGCCTCCTGA

Protein sequence : 
MRYDLAIVGSGGGAFAAAIAARRKGKHVVMVEHGDVGGTCVNTGCVPSKALLAAAHARHVALDAARFPGIRASAEQVDFQ
ELIAGKRRLVADMRADKYVDLAAEYGWEILTGHGRFTEGPLLCVDLPDGGSQRIEAENYLIATGSAPWIPPIDGLAQVGY
LTSTTAMESDHLPQSLLVVGGNYIGLEQGQLFARLGTQVTVVETLDRLAPKEEPEVSAAIEQVFADDGIAVHTTATVRSV
RRENGHVVAVLDGQPGRQIHAEQILIATGRRPVVDGLNLETVGVKTGQHGEILVDEHLRTTNPRIWAAGDVTGHPQFVYV
AGAHGSIVADNAFDNAGRTVDYHHLPQVTFTTPAIASAGMTEAQAISAGHRCDCRVLPLEYVPRAIVNRDTRGLIKLVAD
ADTGRLLGAHVVADAAGEVIATAVYALANDMTVQQMADLWCPYLTMAEGLKLAAQTYTRDVSKLSCCAS

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
SE0085 NP_763640.1 dihydrolipoamide dehydrogenase Not tested SCCpbp4 Protein 2e-94 48
merA BAB47642.1 mercuric reductase Not tested Type-III SCCmec Protein 1e-94 48
merA AAN62178.1 mercuric (Hg(II)) reductase Not tested PAGI-2(C) Protein 2e-72 44
merA ACN81005.1 MerA Hg(II) mercuric ion reductase Not tested AbaR5 Protein 1e-72 43
merA ABQ57371.1 MerA Not tested SGI1 Protein 3e-72 43
merA AGK07021.1 MerA Not tested SGI1 Protein 3e-72 43
merA AGK07079.1 MerA Not tested SGI1 Protein 3e-72 43
merA CAJ77060.1 Mercuric ion reductase Not tested AbaR1 Protein 1e-72 43
unnamed ABR13401.1 mercuric reductase Not tested PAGI-5 Protein 1e-72 42
merA AFG30120.1 MerA Not tested PAGI-2 Protein 7e-71 42
merA YP_006098387.1 mercuric ion reductase Not tested Tn2411 Protein 1e-70 42
merA AET25397.1 MerA Not tested PAGI-2(C) Protein 7e-71 42

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
ML5_4212 YP_004083866.1 mercuric reductase BAC0651 Protein 8e-95 48
ML5_4212 YP_004083866.1 mercuric reductase BAC0224 Protein 2e-91 47
ML5_4212 YP_004083866.1 mercuric reductase BAC0653 Protein 1e-72 45
ML5_4212 YP_004083866.1 mercuric reductase BAC0652 Protein 2e-73 43