Gene Information

Name : Calkr_0526 (Calkr_0526)
Accession : YP_004025687.1
Strain : Caldicellulosiruptor kristjanssonii 177R1B
Genome accession: NC_014721
Putative virulence/resistance : Resistance
Product : d-alanine/d-alanine ligase
Function : -
COG functional category : M : Cell wall/membrane/envelope biogenesis
COG ID : COG1181
EC number : 6.3.2.4
Position : 570133 - 571227 bp
Length : 1095 bp
Strand : +
Note : TIGRFAM: D-alanine/D-alanine ligase; KEGG: ate:Athe_2039 D-alanyl-alanine synthetase A; PFAM: D-alanine--D-alanine ligase domain protein

DNA sequence :
ATGACCAAGTTAAAGGTTGCTGTTTTGTTTGGAGGAGTTTCAACAGAACACGAAATATCTATAGTTTCGGCAAAATCAAT
CATGCAAAATATGGATAAGGAAAAATACGAAATAATTCCAATAGGTATAACAAAAGAAGGAAAATGGCTCTTGTACACAG
GTAAAATTGAAGATTTGGATAACAAGTGGACTCAGTTTTCTATAGAATGTTTTATCTCTCCTGATAGAACCAAAAAGGCA
CTTGTAAAAATAAAAGATAACGAGGCTACCTTTATCGACATTGACGTTGTGTTCCCAGTTTTGCATGGACTGAATGGTGA
GGACGGGACAGTTCAGGGACTTTTAGAGTTGTCTAACATACCGTATGTGGGCTGCGGTGTTCTTTCGTCGGCTATATGCA
TGGACAAAGCATTTGCTAAAAAGCTTGCACTTTTAGAAGGAATACCACAGGGGCATTTTTTGGTTGTATACAAAAACGAA
TATTCAGCTAAAAAAGATTATTTTATAAGGCGAATAGAGAGTGAGTTTTCGTATCCTGTTTTTGTAAAGCCTGCAAACTC
AGGCTCATCTGTTGGTATTTCAAAAGCAAAAGACAGAGAAGACCTTGTTTTAGCAATACATGAGGCTTTTTTGTATGATA
CAAAGATTTTGATTGAACAGGCTATCAACGCTCGTGAGATAGAATGCGCAGTTCTTGGGAATGATGAGGTATTTGTCTCT
GAACCTGGAGAAATAATTCCGTCAAGAGAGTTTTATTCGTATGAGGCAAAGTATATTGATAATTCATCAGAGCTCATCAT
CCCGGCAAGACTTCCAAAAGAAGTTGTTGAAGAGATAAAAGATTTGGCAGCAAGGATTTACAAAATTTTTGAGTGCTGTG
GAATGGCAAGAGTGGACTTTTTTGTTGATAAAGATACGAACAAAGTGTATTTCAACGAGGTAAACACAATACCCGGTTTT
ACAAGTATTTCGATGTATCCAAAACTTATGGAGTTCAGTAGCATTCCATATTCTCAGCTCATTGATAAACTAATTTCTCT
TGCCATTGAGAAGAATGAGCAGAAAAAAAGCATAAAATATAGCAAAGAGGGCTGA

Protein sequence :
MTKLKVAVLFGGVSTEHEISIVSAKSIMQNMDKEKYEIIPIGITKEGKWLLYTGKIEDLDNKWTQFSIECFISPDRTKKA
LVKIKDNEATFIDIDVVFPVLHGLNGEDGTVQGLLELSNIPYVGCGVLSSAICMDKAFAKKLALLEGIPQGHFLVVYKNE
YSAKKDYFIRRIESEFSYPVFVKPANSGSSVGISKAKDREDLVLAIHEAFLYDTKILIEQAINAREIECAVLGNDEVFVS
EPGEIIPSREFYSYEAKYIDNSSELIIPARLPKEVVEEIKDLAARIYKIFECCGMARVDFFVDKDTNKVYFNEVNTIPGF
TSISMYPKLMEFSSIPYSQLIDKLISLAIEKNEQKKSIKYSKEG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
vanB NP_815951.1 D-alanine--D-lactate ligase Not tested Not named Protein 2e-59 44
vanB YP_008394259.1 Vancomycin B-type resistance protein vanB, D-alanine--D-lactate ligase Not tested Not named Protein 2e-58 43

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Calkr_0526 YP_004025687.1 d-alanine/d-alanine ligase U00456.gene.p01 Protein 1e-59 44
Calkr_0526 YP_004025687.1 d-alanine/d-alanine ligase U35369.1.gene6.p01 Protein 1e-59 44
Calkr_0526 YP_004025687.1 d-alanine/d-alanine ligase AE016830.1.gene2250. Protein 1e-59 44
Calkr_0526 YP_004025687.1 d-alanine/d-alanine ligase NC_005054.2598280.p0 Protein 3e-60 43
Calkr_0526 YP_004025687.1 d-alanine/d-alanine ligase NC_014475.1.orf3.gen Protein 3e-60 43
Calkr_0526 YP_004025687.1 d-alanine/d-alanine ligase AF130997.1.orf4.gene Protein 1e-57 43
Calkr_0526 YP_004025687.1 d-alanine/d-alanine ligase AF277571.gene.p01 Protein 8e-58 43
Calkr_0526 YP_004025687.1 d-alanine/d-alanine ligase AF310956.2.orf5.gene Protein 2e-59 43
Calkr_0526 YP_004025687.1 d-alanine/d-alanine ligase AY489045.gene.p01 Protein 2e-55 43
Calkr_0526 YP_004025687.1 d-alanine/d-alanine ligase AY145441.gene.p01 Protein 2e-59 43