Gene Information

Name : Calkro_0619 (Calkro_0619)
Accession : YP_004023332.1
Strain : Caldicellulosiruptor kronotskyensis 2002
Genome accession: NC_014720
Putative virulence/resistance : Resistance
Product : d-alanine/d-alanine ligase
Function : -
COG functional category : M : Cell wall/membrane/envelope biogenesis
COG ID : COG1181
EC number : 6.3.2.4
Position : 766723 - 767817 bp
Length : 1095 bp
Strand : +
Note : TIGRFAM: D-alanine/D-alanine ligase; KEGG: ate:Athe_2039 D-alanyl-alanine synthetase A; PFAM: D-alanine--D-alanine ligase domain protein

DNA sequence :
ATGACTAAGTTAAAAGTTGCTGTTTTGTTTGGAGGAGTTTCAACAGAACACGAAATATCCATAGTTTCGGCAAAATCAAT
TATGCAAAATATGGACAAGGAAAAATACGAAATAATTCCAATAGGTATAACAAAGGAAGGAAAATGGCTCTTGTACACAG
GCAAAATTGAAGATTTGGATAGCAAGTGGACTCAGTTTTCTATAGAATGTTTTATCTCTCCTGATAGAACCAAAAAGGCA
CTTGTAAAAATAAAAGATAACGAGGCTACTTTCATCGACATTGATGTGGTATTTCCGGTTTTGCATGGACTGAATGGTGA
GGACGGAACAGTTCAAGGACTTTTAGAGCTCTCTGGCATACCATATGTAGGGTGCGGTGTTCTTTCGTCAGCTATATGCA
TGGACAAAGCATTTGCTAAAAAGCTTGCGCTTTTAGAAGGAATACCACAGGGTCATTTTTTGGTTGTATACAAAAACGAA
TATTCAGCTAAAAAGGATTATTTCATAAGAAGAATAGAGAGTGAGTTTTCGTATCCTGTTTTTGTAAAGCCTGCAAACTC
AGGTTCGTCTGTGGGTATATCAAAAGCGAAAGATAGAGAAGACCTTGTTTTGGCAATACATGAGGCTTTTTTGTACGATA
CAAAGATTTTGATTGAACAGGCTATAAACGCTCGGGAGATAGAATGTGCAGTTTTAGGAAATGATGAGGTATTTGTGTCT
GAGCCGGGAGAAATAATTCCATCAAGAGAGTTTTATTCGTATGAGGCAAAGTATATTGATAATTCATCAGAGCTTATTAT
TCCTGCAAGACTTCCAAAGGAAGTTACTGAAGAGATAAAAGATTTGGCAGCAAGGATTTACAAAATTTTTGAGTGCTGCG
GAATGGCAAGAGTGGACTTTTTTGTTGATAAAGATACGAACAAAGTGTATTTCAACGAGATAAACACAATACCTGGCTTT
ACAAGTATTTCGATGTATCCAAAGCTTATGGAGTTTAGTAGTATTCCATATTCTCAGCTCATTGATAAACTAATTTCTCT
TGCCATTGAGAAAAATAGACAGAAAAAAAGCATAAAATACAGTAAAGAGGGCTGA

Protein sequence :
MTKLKVAVLFGGVSTEHEISIVSAKSIMQNMDKEKYEIIPIGITKEGKWLLYTGKIEDLDSKWTQFSIECFISPDRTKKA
LVKIKDNEATFIDIDVVFPVLHGLNGEDGTVQGLLELSGIPYVGCGVLSSAICMDKAFAKKLALLEGIPQGHFLVVYKNE
YSAKKDYFIRRIESEFSYPVFVKPANSGSSVGISKAKDREDLVLAIHEAFLYDTKILIEQAINAREIECAVLGNDEVFVS
EPGEIIPSREFYSYEAKYIDNSSELIIPARLPKEVTEEIKDLAARIYKIFECCGMARVDFFVDKDTNKVYFNEINTIPGF
TSISMYPKLMEFSSIPYSQLIDKLISLAIEKNRQKKSIKYSKEG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
vanB NP_815951.1 D-alanine--D-lactate ligase Not tested Not named Protein 6e-60 44
vanB YP_008394259.1 Vancomycin B-type resistance protein vanB, D-alanine--D-lactate ligase Not tested Not named Protein 4e-59 43

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Calkro_0619 YP_004023332.1 d-alanine/d-alanine ligase U35369.1.gene6.p01 Protein 3e-60 44
Calkro_0619 YP_004023332.1 d-alanine/d-alanine ligase AE016830.1.gene2250. Protein 3e-60 44
Calkro_0619 YP_004023332.1 d-alanine/d-alanine ligase U00456.gene.p01 Protein 3e-60 44
Calkro_0619 YP_004023332.1 d-alanine/d-alanine ligase NC_014475.1.orf3.gen Protein 1e-60 43
Calkro_0619 YP_004023332.1 d-alanine/d-alanine ligase NC_005054.2598280.p0 Protein 1e-60 43
Calkro_0619 YP_004023332.1 d-alanine/d-alanine ligase AF277571.gene.p01 Protein 3e-58 43
Calkro_0619 YP_004023332.1 d-alanine/d-alanine ligase AF130997.1.orf4.gene Protein 4e-58 43
Calkro_0619 YP_004023332.1 d-alanine/d-alanine ligase AY489045.gene.p01 Protein 4e-56 43
Calkro_0619 YP_004023332.1 d-alanine/d-alanine ligase AY145441.gene.p01 Protein 6e-60 43
Calkro_0619 YP_004023332.1 d-alanine/d-alanine ligase AF310956.2.orf5.gene Protein 7e-60 43