Gene Information

Name : REQ_08110 (REQ_08110)
Accession : YP_004005611.1
Strain : Rhodococcus equi 103S
Genome accession: NC_014659
Putative virulence/resistance : Virulence
Product : 2,3-dihydroxybenzoate-AMP ligase
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 851226 - 852908 bp
Length : 1683 bp
Strand : -
Note : -

DNA sequence : 
GTGAGCACCGCCCTACGGCGCAGTCCACTGGTCCCCTCCACGCTGTATCCCGCCGAGTTCGCGCAGCGCTACCGGGACGC
CGGGTACTGGACCGACGAGACCTTCGCCCGGTTCCTGCCTGCCCGCGCCGAGCAGTTCGGGGACCGCGTCGCCGTCACCG
GCGCGGGTGAGGGCGACGCCGTGCGCACCCTCACGTACCGTCAGCTGAGCGCCAACGCCGACGTCCTCGCCGCAGGTCTG
CTGCGACTGGGGGTGCGGGCCGGCGACCGGGTCGTACTGCAGCTGCCGAACATGCCCGAGTACGTCGAGATGCTGTTCGC
GGTCTTCACGATCGGGGCCCTGCCGGTGTTCGCGCTGCCCGCGCACCGGCGCGCCGAGGTGAGCTACTTCTGCGAGTTCA
CCGACGCAGCCGCGTACGTGATCGCGGACCGGCACGCCGGGTTCGACTACCGGACGCTGGCGCGGGAGATCGTCGCCGGA
TCCGAGCGTCCGCCGCTGGTGATCGTCGTCGGCGAGGCAGGAGACGATCCCGGCTTCGTCCCGCTCTCCGAGCTGCGGAC
CGCCACACCGGACCCGGGTGACCGCGCCGAACCCGCACCGGACGACGTCGCGTTCCTGCAACTGTCGGGCGGAACCACCG
GGCGTTCCAAGCTCATCCCCCGCACACACACCGACTACCTGTACTCGGTGCGCGCGTCCGCCGAGATCTGCGGCCTCGAC
CCCGACACTCGGATGCTCGTGAGCCTGCCTGCCGCACACAACTTCCCGATGAGCTCGCCCGGCATCCTCGGGGTGCTCCA
CGCGGGCGGGCGCGTGGTGATGGCCCCGGACCCGACGCCGTCGACGTCGTTCGAACTGATCGAGCGCGAGCGGATCACGA
TCGCGTCGCTCGTCCCGCCCCTCGCGTTGACCTGGATGTCGTCGCCGGCCCGCGCGAAGCACGACCTGTCGAGTCTCGAA
CTGCTGCAGGTGGGCGGCGCCAAGTTCGGCGCCGAAGCGGCTCGCCGCGTCCGCGACGAACTCGGGTGCCGGCTGCAGCA
GGTGTTCGGGATGGCCGAAGGCCTGGTGAACTACACGCGCCACGACGATCCCGACGACGTCGTGGTCACCACGCAGGGCC
GGCCGATCAGCCCGGACGACGAGGTGCTCGTGGTGGACGACCACGACAGGCCGGTCGCGGTCGGGGTGGTCGGGCACCTC
CTCACCCGCGGCCCGTACACGATCCGCGGCTACTACGCTGCGCCGGAGCACAATCGGGCGTCGTTCACGGAGGACGGGTT
CTACCGCACCGGTGACCGGGTGCGCCTCACCGAGACCGGGCACATCGTCGTCGAGGGCCGTGCGAAGGACCAGATCAACC
GGGGCGGTGAGAAGGTGGCGGCCGAGGAGGTGGAGAACCACCTGCTCGCGCACCCCGCCGTGCGCGATGTCGCCGTCGTC
TCCAAGCCCGACGCCTACCTGGGCGAGCGCACGTTCGCCTTCGTGGTCGGCGAGGGCACCCCGCCGCGCGCCGTCGAACT
GCGCGTGTTCCTCCGCGAACGCGGACTGGCCGCCTACAAGATCCCCGACGGCTTCCGGTTCGTCGACGAGTTCCCCGTCA
CCGGCGTCGGCAAGGTCAGCCGCACCGAGCTGCGCCGCGCCCTCGAATCCACGTTCGTCACCGAAACCGCTACCGACAGT
TAG

Protein sequence : 
MSTALRRSPLVPSTLYPAEFAQRYRDAGYWTDETFARFLPARAEQFGDRVAVTGAGEGDAVRTLTYRQLSANADVLAAGL
LRLGVRAGDRVVLQLPNMPEYVEMLFAVFTIGALPVFALPAHRRAEVSYFCEFTDAAAYVIADRHAGFDYRTLAREIVAG
SERPPLVIVVGEAGDDPGFVPLSELRTATPDPGDRAEPAPDDVAFLQLSGGTTGRSKLIPRTHTDYLYSVRASAEICGLD
PDTRMLVSLPAAHNFPMSSPGILGVLHAGGRVVMAPDPTPSTSFELIERERITIASLVPPLALTWMSSPARAKHDLSSLE
LLQVGGAKFGAEAARRVRDELGCRLQQVFGMAEGLVNYTRHDDPDDVVVTTQGRPISPDDEVLVVDDHDRPVAVGVVGHL
LTRGPYTIRGYYAAPEHNRASFTEDGFYRTGDRVRLTETGHIVVEGRAKDQINRGGEKVAAEEVENHLLAHPAVRDVAVV
SKPDAYLGERTFAFVVGEGTPPRAVELRVFLRERGLAAYKIPDGFRFVDEFPVTGVGKVSRTELRRALESTFVTETATDS

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 3e-99 46
ybtE CAA21394.1 - Virulence HPI Protein 3e-99 46
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 2e-99 46
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 2e-97 46
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 2e-99 46
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 3e-97 46
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 3e-99 46
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 3e-99 46
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 3e-99 46

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
REQ_08110 YP_004005611.1 2,3-dihydroxybenzoate-AMP ligase VFG0168 Protein 4e-120 51
REQ_08110 YP_004005611.1 2,3-dihydroxybenzoate-AMP ligase VFG0932 Protein 1e-104 47
REQ_08110 YP_004005611.1 2,3-dihydroxybenzoate-AMP ligase VFG1817 Protein 8e-103 46