Gene Information

Name : GY4MC1_0829 (GY4MC1_0829)
Accession : YP_003988255.1
Strain : Geobacillus sp. Y4.1MC1
Genome accession: NC_014650
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : -
Position : 831179 - 832942 bp
Length : 1764 bp
Strand : +
Note : KEGG: gwc:GWCH70_2685 pyruvate kinase; TIGRFAM: pyruvate kinase; PFAM: pyruvate kinase barrel; pyruvate kinase alpha/beta; PEP-utilising protein mobile region

DNA sequence :
ATGATGCGGAAAACGAAAATCGTTTGCACGATTGGTCCTGCTAGCGAAAGCGTGGACAGGCTTGTGGAATTGATCAATGC
TGGAATGAACGTGGCGCGTTTGAATTTTTCCCACGGCGATTATGCAGAACATGGACGCCGCATTCAAAACATTCGCGAAG
CGGTAAAACGGACAGGAAAAACAGTAGCGATCCTTTTAGACACAAAAGGCCCGGAAATTCGCACACATAATATGGAAAAC
GGAGCGATTGAGCTAAAAGAAGGAGAACAGCTTGTCATTTCGATGCAAGAAGTGCTTGGGACACCGGAGAAAATTTCCGT
TACGTATGAAAAGCTAGTTGATGATGTCGCTCCTGGGGCAAAAATTCTTCTCGATGACGGTTTAATCGGGTTAGAAGTGA
TTTCGGTTGATCGGCAAGCGCGTGAAATCGTGACAAAAGTATTAAATGGCGGCGTGCTGAAAAACAAAAAAGGAGTTAAC
GTGCCTGGGGTTCGCGTCAATTTGCCGGGCATTACAGAAAAAGACCGCCAAGACATTTTGTTTGGCATCGAGCAAGGCAT
TGATTTTATCGCTGCTTCATTTGTGCGCCGCGCTTCTGACATATTAGAAATCCGTGAACTGCTTGAGGCAAACGACGCGC
TTCATATTCAAATCATTGCTAAAATCGAAAATCAAGAAGGCGTTGATAATATTGATGAAATTTTAGAAGTCGCGGATGGG
CTTATGGTGGCGCGCGGCGACCTTGGCGTGGAAATTCCAGCTGAAGAAGTGCCGCTTATCCAAAAAGTGCTGATCAAAAA
ATGCAATATGCTTGGTAAACCGGTTATTACGGCTACACAAATGCTTGATTCAATGCAGCGTAACCCAAGACCGACGCGGG
CAGAGGCAAGCGATGTCGCGAATGCGATTTTCGATGGAACAGATGCAGTGATGTTATCTGGAGAAACAGCGGCCGGACAC
TATCCGGTAGAAGCGGTTAAAACCATGCACCAAATTGCTTTGCGCACAGAACAAGCGCTGCAATACCGGGAACTATTAGC
ACAACGGACAAAAGAGAGCGCGACGACGATTACCGATGCAATTGGCCAGTCTGTTGCGCATACGGCGCTGAATCTTGACG
TGGCTGCAATTGTAACGCCGACGGTGAGCGGGAGAACAGCAGGCATGGTGTCAAAATACCGCCCGAAAGCACCGATTGTA
GCGGTTACTGCCGACGAATCGGTATCGCGTAAATTGGCGCTCATATGGGGAGTATATTCGCAAGTTGCCCCACAAGTGAA
CACGACCGATGAAATGCTTGATATTGCCGTAGAAGCGGCGATAAAATCAGGAGTAGTTAAACATGGTGATTTAGTCGTCA
TTACCGCGGGAGTTCCAGTTGGAGAAACGGGATCGACGAACTTGATGAAAGTGCATGTGATCGGGGACATTATCGCGAAA
GGCCAAGGAATCGGCCGTAAGTCTGCGTTCGGAAAGGTGATTATTGCGAACACGGCGGAAGAGGCCATCAACAAAATGGT
AGAAGGCGGAATTTTAGTAACGCACTGCACGGATGCCGACATGATGGAAGCGCTGAAGAAAGCGGCGGCGATCATTACCG
AGGAAGGCGGATTAACGAGCCACGCCGCAGTCGTCGGTCTAAACCTCGGCATTCCTGTTGTTGTCGGTGTAGAAAACGCG
ACATCGATTTTAAAAGACGGGCAGGAGATTACAGTCGATGCCGGATTTGGCGCCGTGTATCAAGGGCATGCAAGCGTGTT
ATAA

Protein sequence :
MMRKTKIVCTIGPASESVDRLVELINAGMNVARLNFSHGDYAEHGRRIQNIREAVKRTGKTVAILLDTKGPEIRTHNMEN
GAIELKEGEQLVISMQEVLGTPEKISVTYEKLVDDVAPGAKILLDDGLIGLEVISVDRQAREIVTKVLNGGVLKNKKGVN
VPGVRVNLPGITEKDRQDILFGIEQGIDFIAASFVRRASDILEIRELLEANDALHIQIIAKIENQEGVDNIDEILEVADG
LMVARGDLGVEIPAEEVPLIQKVLIKKCNMLGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAVMLSGETAAGH
YPVEAVKTMHQIALRTEQALQYRELLAQRTKESATTITDAIGQSVAHTALNLDVAAIVTPTVSGRTAGMVSKYRPKAPIV
AVTADESVSRKLALIWGVYSQVAPQVNTTDEMLDIAVEAAIKSGVVKHGDLVVITAGVPVGETGSTNLMKVHVIGDIIAK
GQGIGRKSAFGKVIIANTAEEAINKMVEGGILVTHCTDADMMEALKKAAAIITEEGGLTSHAAVVGLNLGIPVVVGVENA
TSILKDGQEITVDAGFGAVYQGHASVL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 1e-114 53
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 2e-114 53
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 4e-114 53
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 1e-114 53

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
GY4MC1_0829 YP_003988255.1 pyruvate kinase VFG0479 Protein 6e-115 53