Gene Information

Name : GY4MC1_0169 (GY4MC1_0169)
Accession : YP_003987632.1
Strain : Geobacillus sp. Y4.1MC1
Genome accession: NC_014650
Putative virulence/resistance : Virulence
Product : 2,3-dihydroxybenzoate-AMP ligase
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 183843 - 185468 bp
Length : 1626 bp
Strand : +
Note : KEGG: bsu:BSU31980 2,3-dihydroxybenzoate-AMP ligase; TIGRFAM: 2,3-dihydroxybenzoate-AMP ligase; PFAM: AMP-dependent synthetase and ligase

DNA sequence : 
ATGCTAAGCGGATGCCCAACATGGCCAAAGGAGTTTGCAGACTTTTACCGGAAGGAGGGCTGCTGGGCAGGTGAGACTTT
TGGTGACATGCTGCGGGAAAGGGCGTTAAAGTACGGTGATCGCATTGCAGTTACTTCTGGAGAAAGGCAAATCAGCTATA
AAGAACTGGATAGAAGAGCGGATCAGCTTGCCTCAGGATTCCGTCAACTGGGGATTCGACCAGCGGATCGCGTTGTCGTT
CAATTGCCGAATCGAATTGAATTTTTTGAAGTATGCTTTGCTTTATTCCGTCTGGGGGCGCTGCCTGTCTTTGCCTTGCC
TTCACATCGATATAGTGAAATCAGTTATTTTTGTGAATTTAGCGAAGCGGCGGCTTATATTATTCCAGACCAGCATGGGG
GATTTGATTTCCGCTCCCTTGCCAAGCAGGTGAAGGCTGCTGTTCCAACCCTAAAACACGTCATTGTGGTTGGAGAGGCA
GGAGAGTTTGAACCATTAGAAAATCTGTTTATCGATCCTGTCCAGCTGCCGCTGGTTCATTCAAGCGATGTTGCTTTCTT
CCTGCTGTCCGGAGGAAGCACAGGGCTGCCAAAAATGATTCCGCGGACCCACGATGATTATATTTACAGTCTCCGGGTTA
GTGCCGAGATTTGTAAGCTTCATCAAGATAGTGTTTATCTTGCGGTTCTTCCCATTGCCCATAATTATCCGCTTAGTTCA
CCGGGTGTGCTAGGAACGTTGTATGCCGGCGGAAAAGTGGTCCTTGCAGACAGCCCAAGTCCTGATGAGGCATTTCCGCT
TATTCAGCAAGAGAAAGTCACCATTACTGCGCTCGTGCCGCCGCTTGTTTGGATATGGTTAGATGCCGCATCTTCTTGCA
AGTATGATTTATCCAGCCTTCAAGTTTTGCAAGTCGGCGGGGCGAAATTCAGCGCAGAAGTAGCCAAAAGAGTAAAACCA
GTTCTTGGCTGCACCTTGCAGCAGGTGTATGGCATGGCTGAAGGGCTGGTCAACTATACGAGATTGGATGATCCTGAAGA
ATTGATTATAAATACTCAAGGAAAGCCAATGTCTTACTATGATGAAATCCGCATAGTAGATGAAGAAGATAACGAGGTGG
AGCCTGGCCAAGTTGGAGAGCTGTTAACGAGGGGCCCTTATACCATTCGCGGATATTATAAAGCGGAAGCGCATAATGCG
AAAGCCTTTACTGCGGACGGCTTTTACCGGACGGGCGACTTGGTGCGGATGACTCCATCCGGCTACTTGGTTGTCGAAGG
GCGCGTGAAAGATCAAATTAACCGGGGCGGTGAAAAAATTGCGGCGGAGGAAGTGGAAAATCACCTTTTAGCACATCCTT
CCGTACGTGATGCGGCTGTCGTTGCGATGCCGGATGAATTCCTTGGCGAACGATCGTGCGCTTTTATCATTCTGCGTGAT
CACTCTGTGAAAGCAGCTGAACTGAAGGCGTTTTTGCAAGAGCGCGGAGTGGCAAGTTTTAAAATACCTGATCGCATCGA
ATTCGTTGATTCATTTCCGAAGACAGCGGTTGGAAAAATTAACAAGAGGGCATTACGGCAAATCATTGCCGAAAAACGGC
AATCTGCAGGAAAAACAAACGTATAA

Protein sequence : 
MLSGCPTWPKEFADFYRKEGCWAGETFGDMLRERALKYGDRIAVTSGERQISYKELDRRADQLASGFRQLGIRPADRVVV
QLPNRIEFFEVCFALFRLGALPVFALPSHRYSEISYFCEFSEAAAYIIPDQHGGFDFRSLAKQVKAAVPTLKHVIVVGEA
GEFEPLENLFIDPVQLPLVHSSDVAFFLLSGGSTGLPKMIPRTHDDYIYSLRVSAEICKLHQDSVYLAVLPIAHNYPLSS
PGVLGTLYAGGKVVLADSPSPDEAFPLIQQEKVTITALVPPLVWIWLDAASSCKYDLSSLQVLQVGGAKFSAEVAKRVKP
VLGCTLQQVYGMAEGLVNYTRLDDPEELIINTQGKPMSYYDEIRIVDEEDNEVEPGQVGELLTRGPYTIRGYYKAEAHNA
KAFTADGFYRTGDLVRMTPSGYLVVEGRVKDQINRGGEKIAAEEVENHLLAHPSVRDAAVVAMPDEFLGERSCAFIILRD
HSVKAAELKAFLQERGVASFKIPDRIEFVDSFPKTAVGKINKRALRQIIAEKRQSAGKTNV

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 5e-123 52
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 6e-123 52
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 1e-122 51
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 2e-122 51
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 1e-122 51
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 1e-122 51
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 1e-122 51
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 1e-122 51
ybtE CAA21394.1 - Virulence HPI Protein 1e-122 51

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
GY4MC1_0169 YP_003987632.1 2,3-dihydroxybenzoate-AMP ligase VFG0168 Protein 3e-139 54
GY4MC1_0169 YP_003987632.1 2,3-dihydroxybenzoate-AMP ligase VFG0932 Protein 5e-118 50
GY4MC1_0169 YP_003987632.1 2,3-dihydroxybenzoate-AMP ligase VFG1817 Protein 2e-115 47