Gene Information

Name : Entcl_3201 (Entcl_3201)
Accession : YP_003942731.1
Strain : Enterobacter cloacae SCF1
Genome accession: NC_014618
Putative virulence/resistance : Virulence
Product : 2,3-dihydroxybenzoate-AMP ligase
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 3413380 - 3414987 bp
Length : 1608 bp
Strand : -
Note : KEGG: kva:Kvar_3756 2,3-dihydroxybenzoate-AMP ligase; TIGRFAM: 2,3-dihydroxybenzoate-AMP ligase; PFAM: AMP-dependent synthetase and ligase

DNA sequence : 
ATGAGCATTGCGTTTACCCGCTGGCCCGCTGAGCTTGCGGCCCGCTACCGTGAAAAAGGGTACTGGCAGGATCTGCCGCT
GACCGACATTCTCACCCGCCATGCCGACAGCGACGCGCTGGCGGTGATTGAGGGCGAGCGCGCGCTGAGCTACCGGACGC
TGAACCAGCAGGTGGATAACCTGGCCTGTCAGCTGGCGCGCCTCGGCGTGAAGGCGGGCGAAACGGCGCTGGTGCAGCTT
GGCAACGTGACGGAGTTTTATGTCACGCTGTTTGCGCTGCTGCGTCTTGGCGTGGCGCCGGTTAACGCGCTGTTCAGCCA
TCAGCGCAGCGAGCTGAACGCCTATGCGGCGCAAATCGCGCCGGCGCTGCTGATCGCCGACCGCGAGCACGCGCTGTTCG
CCGATGAGGCGTTCCTGCAGGATTTTATGGCCGAACACCGCTGCGTGCGGACGGTGCTGCTGCGCAACGACGACGGCGAG
CAGAGCCTGGCGGCGGCTATCGCCCGTCCGGCAGAGCGTTTTGCTCCCGCGCCGTCGCCAGCCGATGAGGTGGCGTTTTT
CCAGCTCTCCGGCGGCAGCACCGGGACGCCAAAGCTGATCCCCCGTACGCACAACGACTACTACTACAGCATTCGCCGCA
GCAACGAGATTTGCGCCTTCGGCCCGCACACCCGCTACCTGTGCGCGCTTCCGGCGGCGCACAACTATCCGCTGAGCTCG
CCCGGCGCGCTGGGCGTGTTTCTCGCCGGCGGTCGGGTGGTGCTGGCCTCTGATCCGAGCGCCACGCAGTGCTTCCCGTT
GATTGAGAAGCACCAGATAACCGTCACCGCGCTGGTGCCGCCCGCGGTCAGCCTGTGGCTGCAGGCGGTTCAGGAGGGCG
GCAATGCGCCGCTGCGCTCGCTCACGCTGCTGCAGGTGGGCGGCGCCCGGCTGTCGGCAACCCTGGCCGCGCGCATTCCC
GCCGCGATCGGCTGCCAGCTGCAGCAGGTGTTCGGTATGGCCGAAGGGCTGGTGAACTACACCCGGCTCGACGACCCGCT
GGAGCGCATTATCCACACTCAGGGCTGCCCGATGAGCCCGGATGATGAGGTGTGGGTCGCCGGTGAGCGCGGCGAACCGC
TGCCGCGCGGCGAGGTGGGGCGTCTGATGACCCGCGGACCGTACACCTTTCGCGGCTACTACAACAGCCCGGCGCATAAC
GCCAGCGCGTTTAACGCCGACGGCTTTTACTGCTCCGGCGACCTGATTTCCATGGATCAGCACGGCTACATCACCGTGCA
GGGGCGCGAGAAAGATCAGATCAACCGCGGCGGTGAAAAAATTGCCGCCGAAGAGGTGGAAAACCTGCTGCTGCGCCATG
AGGCGGTGATTCATGCGGCGCTGGTGAGCATGGAGGACAGCCTGCTGGGTGAAAAAAGCTGCGCGTATCTGGTGGTGAAT
GCGCCGCTGCGCGCGGTCGCCGTACGCCGGTTCCTGCGCGAGCAGGGCGTGGCGGAGTTTAAGCTGCCGGATCGGGTGGA
GTGCGTCGACGCGCTGCCGCTGACGCCGGTCGGCAAAGTGGATAAGAAAAAACTGCGTCAGCGCCTGGCAGAACGCCCGC
TGGCCTGA

Protein sequence : 
MSIAFTRWPAELAARYREKGYWQDLPLTDILTRHADSDALAVIEGERALSYRTLNQQVDNLACQLARLGVKAGETALVQL
GNVTEFYVTLFALLRLGVAPVNALFSHQRSELNAYAAQIAPALLIADREHALFADEAFLQDFMAEHRCVRTVLLRNDDGE
QSLAAAIARPAERFAPAPSPADEVAFFQLSGGSTGTPKLIPRTHNDYYYSIRRSNEICAFGPHTRYLCALPAAHNYPLSS
PGALGVFLAGGRVVLASDPSATQCFPLIEKHQITVTALVPPAVSLWLQAVQEGGNAPLRSLTLLQVGGARLSATLAARIP
AAIGCQLQQVFGMAEGLVNYTRLDDPLERIIHTQGCPMSPDDEVWVAGERGEPLPRGEVGRLMTRGPYTFRGYYNSPAHN
ASAFNADGFYCSGDLISMDQHGYITVQGREKDQINRGGEKIAAEEVENLLLRHEAVIHAALVSMEDSLLGEKSCAYLVVN
APLRAVAVRRFLREQGVAEFKLPDRVECVDALPLTPVGKVDKKKLRQRLAERPLA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 7e-93 44
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 6e-93 44
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 4e-94 43
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 6e-94 43
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-94 43
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 5e-94 43
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-94 43
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-94 43
ybtE CAA21394.1 - Virulence HPI Protein 5e-94 43

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Entcl_3201 YP_003942731.1 2,3-dihydroxybenzoate-AMP ligase VFG0932 Protein 0.0 80
Entcl_3201 YP_003942731.1 2,3-dihydroxybenzoate-AMP ligase VFG0168 Protein 1e-109 47
Entcl_3201 YP_003942731.1 2,3-dihydroxybenzoate-AMP ligase VFG1817 Protein 6e-86 42