Gene Information

Name : pykF (CLOST_1647)
Accession : YP_003936672.1
Strain : Clostridium sticklandii DSM 519
Genome accession: NC_014614
Putative virulence/resistance : Virulence
Product : pyruvate kinase I
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 1779186 - 1780937 bp
Length : 1752 bp
Strand : -
Note : Evidence 2a : Function of homologous gene experimentally demonstrated in an other organism; PubMedId : 10751408, 1859631, 8591049, 89228557, 89386643, 9023191, 9298646; Product type e : enzyme

DNA sequence :
ATGTTCAATAAAAAAACAAAAATAGTATGCACTATAGGACCAGCTAGTGAAAGCATAGATACGCTTAAGGAGCTTATAAA
AAGCGGTCTAAATGTATGTAGACTTAATTTCTCTCACGGTAATTATGAAGAGCATGGAAAGAGAATAGATAACATAAAAG
CAGCTAGAAATGAAATGAAGCTGCCAATTGCAATACTGCTTGATACTAAAGGCCCTGAAATTAGAACAGGTAAATTTTCA
TCTCCAGAAGTAAATCTAGTAGAAGGACAAAACTTCATCATTACTATGGAAGAGGTTTTAGGCGATGAAACAAAGTGTAC
AGTTTCATATAAAGAACTAGTTAACGATGTAAAGCCTGGCAACCAAATTCTTATAGACGATGGTCTAGTAGGTCTTGCGG
TACAAGAAATCAAAGGACAAGAGATACTATGCATCGTTCAAAATGCAGGCACTATCAAAGACAATAAAGGCGTAAACGTA
CCTAATGTAAAAATAAATCTTCCTGCAATAACAGCAAAAGATAAAAAAGACATAGAATTTGGTATTGAACAAGGAATTGA
TTTTATTGCTGCTTCATTTGTGCGTAAAGCATCTGACGTGCTAGCTATAAGAGAAATACTAGAAGAACATAATGCTACTA
ACATACAGATTATTTCAAAGATAGAAAACCAAGAAGGCGTAGACAACATAGACGAAATATTAGAGGTTTCTGACGGCTTG
ATGGTTGCAAGAGGTGATTTGGGAGTTGAAATCCCAACTGAAGACATTCCTATAGTTCAAAAAGAGTTAATCAAAAAATG
TAATATACTTGGAAAGCCAGTAATAACAGCTACACAAATGTTAGATTCAATGATAAGAAACCCAAGACCTACAAGAGCAG
AGGTTACAGATGTTGCTAATGCAATATTTGATGGTACAGATGCTATCATGCTATCTGGAGAAACAGCAGCTGGAAAGTAT
CCACTTGAATCTGTTAAGACTATGGCATCTATAGCTATAAGGGCAGAGCAAACCTTAGATTATGAAGAATTACTAAAAAC
TAAAGTTAAGCTACGCCAGCTAAACATCACAAACGCTATCAGTCATGCGACTTGTACGACTGCAATTGATTTAAAAGCAA
GTGCAATTATATCAGCTACAGCTTCTGGCTACACTGCTAGAATGGTATCTAGCTATAGACCATCCGCTCCAATAATTGCG
GCAACAAACTCTGAAATGGTTATGAGACAAATGGGTCTTGTTTGGGGGGTGTATCCTCTACTTACTGAAAAAGGAATGTC
TACAGATGATGTATTCGAAAAATCAGTTCAAAGTGCTTTAGACATGGATTATATCTCATCTGGAGACCTTGTAGTAATAA
CGGCTGGTGTTCCTGTAGGAATAGCAGGTACAACAAACCTAATCAATGTCCATATTGCTTCTGAAATACTTATAAGAGGA
GTAGGTGTTGGAACTACAAATATTTCTGGTAGAGCTAGACTAATTACTGATTCTAATATGGAAATAGAACAAGGTGATAT
TATAGTAGCATCTAGCACAGATAAAGATATGATGAATATAATAAACAAGGCATCTGCAATTATTACAGAAGAAGGCGGTC
TTACTAGCCATGCTGCTGTAGTGGGAGTGAGCCTAGGAATACCTGTAATAGTATCTGCTACAAATGCTCTAAAGATAATA
GAAGATAATGAAACTATAACTATAGATGCTCAAAGCGGATTAGTATATAAAGGCGAAGTAAGAATGCTATAA

Protein sequence :
MFNKKTKIVCTIGPASESIDTLKELIKSGLNVCRLNFSHGNYEEHGKRIDNIKAARNEMKLPIAILLDTKGPEIRTGKFS
SPEVNLVEGQNFIITMEEVLGDETKCTVSYKELVNDVKPGNQILIDDGLVGLAVQEIKGQEILCIVQNAGTIKDNKGVNV
PNVKINLPAITAKDKKDIEFGIEQGIDFIAASFVRKASDVLAIREILEEHNATNIQIISKIENQEGVDNIDEILEVSDGL
MVARGDLGVEIPTEDIPIVQKELIKKCNILGKPVITATQMLDSMIRNPRPTRAEVTDVANAIFDGTDAIMLSGETAAGKY
PLESVKTMASIAIRAEQTLDYEELLKTKVKLRQLNITNAISHATCTTAIDLKASAIISATASGYTARMVSSYRPSAPIIA
ATNSEMVMRQMGLVWGVYPLLTEKGMSTDDVFEKSVQSALDMDYISSGDLVVITAGVPVGIAGTTNLINVHIASEILIRG
VGVGTTNISGRARLITDSNMEIEQGDIIVASSTDKDMMNIINKASAIITEEGGLTSHAAVVGVSLGIPVIVSATNALKII
EDNETITIDAQSGLVYKGEVRML

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 1e-104 52
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 1e-104 52
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 2e-105 52
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 5e-104 52

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pykF YP_003936672.1 pyruvate kinase I VFG0479 Protein 5e-105 52