Gene Information

Name : hrcN (Dda3937_00611)
Accession : YP_003883242.1
Strain : Dickeya dadantii 3937
Genome accession: NC_014500
Putative virulence/resistance : Virulence
Product : type III secretory pathway, ATPase HrcN
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : -
Position : 2655406 - 2656788 bp
Length : 1383 bp
Strand : +
Note : E. coli containing D. dadantii hrp cluster is capable of secreting an exogenous effector into tobacco, eliciting a hypersensitive response

DNA sequence :
GTGATGAACGCGCAGGACGCAATCGATACCCGCTACCCCCAGCTGAGCCGGTGGCTGCAACAGCAACAACGTCAGCTGGC
GGCGTATTCGCCGGTGACGCGACAGGGGCAAATCATCGGCATCAGCGGCATTCTGCTGGAGTCCAGCCTGCCGAACGCCC
GCATCGGCGATCTGTGCCTGGTGGCGCGCGACGACAGCTCGCAGGTGCTGGCGGAAATTGTCGGCTTCAGCCCGGAAAAC
ACCTTTCTGTCGGCGCTGGGCGCGCTGGACGGCATCGCGCAGGGCGCGGTGGTCACCCCGCTCTACCAGCCGCATTGCGT
GCAGGTGTCCGACGCGCTGTTCGGCAGTGTGCTGGACGGCTTCGGCCGCCCGCTGGAAGACGGCGGCCACAGCGCGTTTG
TCGAGCCAGGCTCACTTAACGCCACCCCTATCCCGGTGATCGGCGACGCGCCGCCGCCTACTGAACGCCCGCGCATCGCC
GACCCGCTGCCCACCGGCATGCGCGCCATCGACGGCCTGCTGACCATCGGCTGCGGTCAGCGTGTCGGAGTATTCGCCGG
CGCCGGCTGCGGCAAAACCACCCTGCTGGCGGAGCTGGCGCGCAACACCCCGTGCGACGCCATCGTGTTTGGGCTGATCG
GCGAACGCGGCCGCGAACTGCGCGAATTCCTCGATCACGAACTGGACGAACAGCTGCGCAGCCGCACCGTGCTGGTCTGC
TCCACCTCCGATCGCAGCAGCATGGAGCGCGCCCGCGCCGCCTTTACCGCTACCGCCATCGCCGAAGCCTTTCGCGCCGC
GGGCAAACAAGTGCTGCTGATTATCGACTCGCTGACCCGCTTTGCACGCGCTCAGCGCGAAATCGGCCTGGCGCTGGGCG
AGCCGCAGGGGCGCGGCGGCTTGCCGCCCTCGGTCTACACCCTGTTGCCCCGGCTGGTGGAACGCGCCGGGCAAACCCGT
CAGGGCGCGATTACCGCGCTCTATTCGGTGCTGATCGAACAGGATTCGATGAACGACCCGGTGGCCGACGAAGTGCGATC
GCTGATCGACGGCCACATCGTGTTGTCGCGCCGGCTGGCCGAACGCAACCACTACCCGGCGATCGACGTGCTGATGAGCC
TGAGCCGCACCATGAGCAACGTGGTGGAACGGGATCACACCCGTCACGCCAACGCGGTACGGCGGCTGATGGCGGCCTAC
AAACAGGTGGAAATGCTGATCCGCCTCGGGGAATACCAACCGGGGCACGACGCCTTCACCGATGCCGCCGTCAGCGCCAA
CGACGACATCAACCGCTTTCTGCAACAGGCGATGCGCGAGCCGCAGGGCTTTGACCACATTCAGTCGCAACTCGAAGAGG
TGAGCCAGTATGCCAATCTCTAA

Protein sequence :
MMNAQDAIDTRYPQLSRWLQQQQRQLAAYSPVTRQGQIIGISGILLESSLPNARIGDLCLVARDDSSQVLAEIVGFSPEN
TFLSALGALDGIAQGAVVTPLYQPHCVQVSDALFGSVLDGFGRPLEDGGHSAFVEPGSLNATPIPVIGDAPPPTERPRIA
DPLPTGMRAIDGLLTIGCGQRVGVFAGAGCGKTTLLAELARNTPCDAIVFGLIGERGRELREFLDHELDEQLRSRTVLVC
STSDRSSMERARAAFTATAIAEAFRAAGKQVLLIIDSLTRFARAQREIGLALGEPQGRGGLPPSVYTLLPRLVERAGQTR
QGAITALYSVLIEQDSMNDPVADEVRSLIDGHIVLSRRLAERNHYPAIDVLMSLSRTMSNVVERDHTRHANAVRRLMAAY
KQVEMLIRLGEYQPGHDAFTDAAVSANDDINRFLQQAMREPQGFDHIQSQLEEVSQYANL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 8e-147 82
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 3e-133 74
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 1e-133 74
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 1e-132 74
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 3e-132 73
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 9e-116 71
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 6e-116 71
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 1e-119 71
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 1e-119 71
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 2e-108 70
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 2e-106 66
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 2e-106 66
lscN AAO18044.1 LscN Virulence TTSS locus Protein 2e-87 52
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 6e-80 50
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 5e-80 50
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-80 50
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 3e-81 50
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-81 50
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 1e-81 50
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 1e-81 50
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 5e-79 50
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 5e-79 50
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 9e-73 49
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 1e-72 49
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-73 49
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 1e-72 49
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 9e-73 49
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 7e-76 49
escN CAI43874.1 EscN protein Virulence LEE Protein 3e-74 47
escN YP_003236088.1 translocator EscN Virulence LEE Protein 2e-73 47
escN NP_290268.1 hypothetical protein Virulence LEE Protein 9e-74 47
escN AAC38383.1 EscN Virulence LEE Protein 8e-74 47
ECs4568 NP_312595.1 EscN Virulence LEE Protein 9e-74 47
escN AAC31513.1 L0034 Virulence LEE Protein 6e-74 47
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 6e-74 47
escN YP_003223475.1 translocator EscN Virulence LEE Protein 4e-74 47
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 7e-73 46
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 7e-73 46
escN AAK26715.1 EscN Virulence LEE Protein 3e-74 46
unnamed AAL06369.1 EscN Virulence LEE Protein 2e-72 46
escN AAL57542.1 EscN Virulence LEE Protein 3e-74 46
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 6e-74 46
escN CAC81862.1 EscN protein Virulence LEE II Protein 4e-74 46
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 4e-63 44
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 4e-63 44
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 4e-63 44
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 4e-63 44
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 2e-60 43
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 1e-62 43
invC AAS66861.1 InvC Not tested SSR-2 Protein 1e-59 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
hrcN YP_003883242.1 type III secretory pathway, ATPase HrcN VFG0390 Protein 2e-86 53
hrcN YP_003883242.1 type III secretory pathway, ATPase HrcN VFG0192 Protein 1e-86 52
hrcN YP_003883242.1 type III secretory pathway, ATPase HrcN VFG0048 Protein 4e-87 51
hrcN YP_003883242.1 type III secretory pathway, ATPase HrcN VFG0515 Protein 6e-73 49
hrcN YP_003883242.1 type III secretory pathway, ATPase HrcN VFG0730 Protein 5e-74 47
hrcN YP_003883242.1 type III secretory pathway, ATPase HrcN VFG0812 Protein 4e-74 47
hrcN YP_003883242.1 type III secretory pathway, ATPase HrcN VFG2144 Protein 3e-82 46
hrcN YP_003883242.1 type III secretory pathway, ATPase HrcN VFG1254 Protein 1e-70 46
hrcN YP_003883242.1 type III secretory pathway, ATPase HrcN VFG0555 Protein 2e-63 44
hrcN YP_003883242.1 type III secretory pathway, ATPase HrcN VFG1778 Protein 1e-62 44
hrcN YP_003883242.1 type III secretory pathway, ATPase HrcN VFG2499 Protein 3e-65 44
hrcN YP_003883242.1 type III secretory pathway, ATPase HrcN VFG2459 Protein 4e-64 42
hrcN YP_003883242.1 type III secretory pathway, ATPase HrcN VFG1008 Protein 6e-59 42
hrcN YP_003883242.1 type III secretory pathway, ATPase HrcN VFG2331 Protein 3e-66 42