Gene Information

Name : hrcV (Dda3937_00609)
Accession : YP_003883240.1
Strain : Dickeya dadantii 3937
Genome accession: NC_014500
Putative virulence/resistance : Virulence
Product : type III secretion inner membrane channel protein (LcrD,HrcV,EscV,SsaV)
Function : -
COG functional category : U : Intracellular trafficking, secretion and vesicular transport
COG ID : COG4789
EC number : -
Position : 2652324 - 2654426 bp
Length : 2103 bp
Strand : +
Note : E. coli containing D. dadantii hrp cluster is capable of secreting an exogenous effector into tobacco, eliciting a hypersensitive response

DNA sequence :
ATGGCTGTGCTGATCGCCTGGCTTAACCGTTTTGCCATGAGCGCCATGCAGCGCTCCGAAGTGGTGGGCGCCGTCATCGT
CATGGCGATTGTGTTCATGATGATTATCCCGCTGCCGACCGGCCTGATTGACGTGCTGATTGCGTTCAACATCTGTATTT
CATCACTGCTGATCGTGCTGGCGATGTACCTGCCCAAACCGCTGGCGTTCTCTACCTTCCCGGCGGTGCTGCTGCTGACC
ACCATGTTCCGGCTGGCGCTGTCCATCTCCACCACCCGGCAGATTCTGTTGCAACAGGATGCCGGTCACGTGGTGGAGGC
GTTCGGCAACTTCGTGGTGGGCGGCAATCTGGCGGTCGGCCTGGTGATCTTCATGATCCTGACGGTGGTGAACTTTCTGG
TGATCACCAAAGGGTCGGAGCGGGTGGCGGAAGTGGCGGCGCGTTTCACCCTCGATGCCATGCCCGGCAAACAGATGTCC
ATCGACAGCGACCTGCGCGCCGGGTTGATCGACGCCCAACAGGCTCGCCAGCGACGGGAAAACCTGGCCAAGGAGAGCCA
ACTGTTCGGTGCCATGGACGGGGCGATGAAGTTCGTCAAAGGGGATGCCATCGCCAGCCTGGTGATCGTATTCATCAACA
TGATTGGCGGCTTCGCCATCGGCGTGTTGCAAAACGGCATGGCCGCCGGCGACGCCATGCACATTTACTCGGTGCTGACC
ATCGGCGACGGCCTGATCGCTCAGATTCCGGCGCTGCTGATTTCGCTGACCGCCGGGATGATCATCACCCGCGTCTCCGC
CGACGGCCAGAAAATGGACAACAATATTGGCCGGGAGATCGCCGAGCAGCTGACCAGCCAGCCCAAGGCGTGGATCATCT
CGTCGGTGGGCATGTTGGGGTTCGCGCTGCTGCCCGGTATGCCGACGCTGGTGTTTCTGCTAATCAGCCTGGTGTCGCTC
GGCAGCGGGTTGTTTCAGCTGTGGCGCGTCAAACAGAGCGGCTTGCAGGATTCGCTGCTGGCGGATGACGGCATGCCCGC
TGAACAGAACGGCTATCAGGATCTGCGGCGTTTCAACCCGACCCGCGCCTACCTGCTGCAGTTCCATACCGTGTGGCAGG
GCGCCGCGGCGGCCGCGGTGCTGGTACAGGACATCCGTCGCCTGCGCAACCGGCTGGTGTACCACTTCGGCTTTACGCTG
CCGTCGTTCGATATCGAATTCAACCCCAGCGTGCCGGAGGATGAGTTCCGCTTCTGCGTGTATGAAATCCCGCAGTTGCG
AGCCAGCTTCGGCGTGCCGCTGCTGGCGGTGCCGCGCGGACAACTGCCGGAAGCGATGCAGGACGACGCCATGATGCCCG
GCCTGTCGGCCCGCGACGAACACCACCTGCTGTGGCTGACGCCGGAACATCCGCTATTACAGCAGCCGGAACTGAGCCCC
TGGAGCCCGGACGCGCTGATCCTCAGTCGCATGGAGAATGCGATTCATCGCAGCGGCGCGCAGTTTATCGGCCTGCAGGA
AACCAAATCGATCCTCGCCTGGCTGGAGGCCGAACAGCCGGAGCTGGCGCAGGAGCTACAGCGCATCATGCCGTTATCGC
GCTTCGCCAGCGTACTGCAACGGCTGGCGTCGGAGCGGGTGCCGCTGCGTTCGGTGCGGCCGATCGCCGAAGCGCTGATC
GAAATCGGTCAGCATGAGCGCGACATCAACGCCCTCACCGACTACGTGCGGCTGGAGCTGAAAGCCCAAATTTGCCACCA
GTACAGTCAGGACGACAGCCTCACCGTCTGGCTGCTGACGCCGGAAACCGAAGAGCTGCTGCGCGACGCGCTGCGCCAGA
CGCAGAACGAAACCTTCTTCGCGCTGACCCAGGAATACGCCGCCACCCTGCTCGGCCTATTGCGGCGCGCATTCCCGCCG
ATGACGCCGCCGTCGGCGCTGATTCTGGTGGCGCAGGACCTGCGCAGCCCGCTGCGCATTCTGTTGCAGGACGAATTTCA
TCACGTACCGGTGCTGTCGTTCACCGAACTGGAGTCGCACCTGTCGATCAACGTGGCGGGCCGTATCGACCTGCTGGACC
GGGTTGACCCCTTTAACGCATAG

Protein sequence :
MAVLIAWLNRFAMSAMQRSEVVGAVIVMAIVFMMIIPLPTGLIDVLIAFNICISSLLIVLAMYLPKPLAFSTFPAVLLLT
TMFRLALSISTTRQILLQQDAGHVVEAFGNFVVGGNLAVGLVIFMILTVVNFLVITKGSERVAEVAARFTLDAMPGKQMS
IDSDLRAGLIDAQQARQRRENLAKESQLFGAMDGAMKFVKGDAIASLVIVFINMIGGFAIGVLQNGMAAGDAMHIYSVLT
IGDGLIAQIPALLISLTAGMIITRVSADGQKMDNNIGREIAEQLTSQPKAWIISSVGMLGFALLPGMPTLVFLLISLVSL
GSGLFQLWRVKQSGLQDSLLADDGMPAEQNGYQDLRRFNPTRAYLLQFHTVWQGAAAAAVLVQDIRRLRNRLVYHFGFTL
PSFDIEFNPSVPEDEFRFCVYEIPQLRASFGVPLLAVPRGQLPEAMQDDAMMPGLSARDEHHLLWLTPEHPLLQQPELSP
WSPDALILSRMENAIHRSGAQFIGLQETKSILAWLEAEQPELAQELQRIMPLSRFASVLQRLASERVPLRSVRPIAEALI
EIGQHERDINALTDYVRLELKAQICHQYSQDDSLTVWLLTPETEELLRDALRQTQNETFFALTQEYAATLLGLLRRAFPP
MTPPSALILVAQDLRSPLRILLQDEFHHVPVLSFTELESHLSINVAGRIDLLDRVDPFNA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
hrcV AAB05999.2 HrcV Virulence Hrp PAI Protein 0.0 78
hrcV AAT96337.1 HrcV Virulence S-PAI Protein 0.0 69
hrcV AAT96256.1 HrcV Virulence S-PAI Protein 0.0 69
hrcV AAT96297.1 HrcV Virulence S-PAI Protein 0.0 69
hrcV ABA47273.1 HrcV Virulence S-PAI Protein 0.0 69
hrcV ABQ88352.1 HrcV Virulence Hrp PAI Protein 0.0 65
hrpI AAA03015.1 HrpI Virulence Hrp PAI Protein 0.0 65
hrcV NP_791229.1 type III secretion protein HrcV Virulence Hrp PAI Protein 0.0 64
hrcV AAG33877.1 HrcV Virulence Hrp PAI Protein 0.0 64
hrcV AAT96139.1 HrcV Virulence T-PAI Protein 0.0 64
hrcV AAT96193.1 HrcV Virulence T-PAI Protein 0.0 64
hrcV AAR25631.1 type III secretion protein HrcV Not tested Not named Protein 2e-179 63
lssD AAO18050.1 LssD Virulence TTSS locus Protein 2e-114 42
invA AAS66860.1 InvA Not tested SSR-2 Protein 1e-95 41
YPO0266 YP_002345347.1 secretion system apparatus protein SsaV Virulence Not named Protein 3e-95 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
hrcV YP_003883240.1 type III secretion inner membrane channel protein (LcrD,HrcV,EscV,SsaV) VFG0064 Protein 4e-116 43
hrcV YP_003883240.1 type III secretion inner membrane channel protein (LcrD,HrcV,EscV,SsaV) VFG0384 Protein 1e-113 42
hrcV YP_003883240.1 type III secretion inner membrane channel protein (LcrD,HrcV,EscV,SsaV) VFG0198 Protein 3e-104 42