Gene Information

Name : pyk1 (PPE_00286)
Accession : YP_003868706.1
Strain : Paenibacillus polymyxa E681
Genome accession: NC_014483
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 323931 - 325346 bp
Length : 1416 bp
Strand : +
Note : TIGRFAMsMatches:TIGR01064; COGMatches:COG0469; PfamMatches:PF00224, PF02887; go_process: glycolysis (GO:0006096)

DNA sequence : 
ATGCTTAAAACAAAAATTATTTGTACTATGGGACCTGCCTGTGACTCCATTCATTTGTTAAAAGAAATGATCCAGGCTGG
TATGACGGTAGCCCGCTTGAATATGGCTCACGGTGAACTGGAAGATCACGTTAAACGTATCGAAAATGTGCGTCAGGCTG
CGAAGGAAATGAATACCTTTATCCCTGTCATGATGGATATTAAAGGACCGGAAATACGGATTGGCAAGCTGAGAGAGGCA
TCTTGTCTGCTGAAACCAGGTAGCCAGCTCATTCTGACCACAGAAGAGATTCTAGGGGACGAGCATCGTATTTCAGTGAA
TTACCCGGATATGCCGAAGGACATCAAACCAGGCGACCGCATTCTCATTGACGACGGTTTGGTGGATCTGACTGTAACCT
CCATCGAAGGTACGGAAATGATTTGTAATATTGTAAGTGGCGGTATTTTGAAGCCGAGAAAAGGTGTGAACCTGCCAGGG
ATTCATACGACCCTTCCAGGCGTGACGGAGCGAGATGTGAAGCACATCCAATTTGGGGTGGAGCAGCGTGTTGAGCTTAT
TGCGGCTTCTTTTGTGCGTAAAGGCGACGATATCCGTGAAATCCGTGAGATTTTGAAGGGACATCATGCTGAGCATGTAC
AAATTTATTCCAAGATTGAAAATGCTGAAGGCATGGAAAACCTGGATGATATTATCGAAGCTTCTGATGGCATTATGGTA
GCCCGTGGCGATCTGGGTGTCGAAGTGCCGATTCAAGATGTGCCTGTGATGCAAAAAGAAATGATCGACAAGTGTAATCT
GGTCGGCAAGCCCGTTATCGTAGCTACCCACATGCTGGAATCCATGCAGGTGAACCCGCGTCCGACGAGAGCCGAAGTTA
GTGACGTGTTTAATGCCGTTATGCAAGGAGCCGATGTTGTTATGCTGTCGGGCGAATCAGCTGCGGGTAAATATCCTGTC
GAGTCTGTACGGACCATGGCCGCTGTAGCTGCCAGAGCAGAATCGCAATTCGATTATCAGGAGCAGTTCGATAAGCGCAG
AGCACGTCAAAGCACCAACATTACGGAAGTGATTAGCCAGGGGGCGGTGAGTTCGTCCCTGGAGCTGGATGCGAAAGCGA
TTATTACGTCGACTGCCAGCGGATTCACAGCACGTATGGTATCCAAATATCGTCCGAAGGCACCTATTATTGCGGTTACT
CCGAATAATACGGTATTCGCTAAAATTTGCCTGTTATCCGGTGTGTTCCCGATCAAAGGGGATAAAGTAGCTACAACGGA
TGAAATGTTCGAATCCTCCATCCGTAACGCCCTGAATGCAGGATATATCCATAAAGGGGATACTGTAGTCCTGTCGGCAG
GTGTTCCAGTCGCTGAGGCAGGTACTACGAACTTGATCCGAGTATATCAAGTGTAA

Protein sequence : 
MLKTKIICTMGPACDSIHLLKEMIQAGMTVARLNMAHGELEDHVKRIENVRQAAKEMNTFIPVMMDIKGPEIRIGKLREA
SCLLKPGSQLILTTEEILGDEHRISVNYPDMPKDIKPGDRILIDDGLVDLTVTSIEGTEMICNIVSGGILKPRKGVNLPG
IHTTLPGVTERDVKHIQFGVEQRVELIAASFVRKGDDIREIREILKGHHAEHVQIYSKIENAEGMENLDDIIEASDGIMV
ARGDLGVEVPIQDVPVMQKEMIDKCNLVGKPVIVATHMLESMQVNPRPTRAEVSDVFNAVMQGADVVMLSGESAAGKYPV
ESVRTMAAVAARAESQFDYQEQFDKRRARQSTNITEVISQGAVSSSLELDAKAIITSTASGFTARMVSKYRPKAPIIAVT
PNNTVFAKICLLSGVFPIKGDKVATTDEMFESSIRNALNAGYIHKGDTVVLSAGVPVAEAGTTNLIRVYQV

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 2e-92 45
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 7e-93 45
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 7e-93 45
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 5e-92 45

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pyk1 YP_003868706.1 pyruvate kinase VFG0479 Protein 3e-93 45