Gene Information

Name : Galf_2176 (Galf_2176)
Accession : YP_003847945.1
Strain : Gallionella capsiferriformans ES-2
Genome accession: NC_014394
Putative virulence/resistance : Resistance
Product : cadmium-translocating P-type ATPase
Function : -
COG functional category : P : Inorganic ion transport and metabolism
COG ID : COG2217
EC number : -
Position : 2356495 - 2358678 bp
Length : 2184 bp
Strand : -
Note : KEGG: mms:mma_1796 cation-translocating P-type ATPase; TIGRFAM: cadmium-translocating P-type ATPase; ATPase, P-type (transporting), HAD superfamily, subfamily IC; heavy metal translocating P-type ATPase; PFAM: E1-E2 ATPase-associated domain protein; Heavy

DNA sequence :
ATGAAACACTCAATGTTCAATATTCGTAACATGGACTGTCCAACTGAGGAGGCACTGATCCGCAAGCGTCTCGGATCGAT
GCCCGGCATCGGCGAGCTGTCGTTCAACCTGATAACCCGCCGTCTTGCCGTCACGCATACACTGGACGATGAACAGCCTA
TCCTCGATGCCTTGCGCGAGATAGGTATGAAGACAGACCCCGAGCCGCAATGCCAGTCCGGCTCCTGTTCGAGCTGCGAA
GCGGAATCCCCCGTGGTATCGCGCCGGACATGGGCGCTGATGGCGGTGTCCGGTACTGCGGCCATGGTTGCGGAAATCGT
CGCGTGGAACGGCGCGAGCGAGCAATCGGTCGCGGTGATCGCTCTGGCCTTGCTCTCCATTGTCACCGGTGGCCTCAGCA
CTCTGAAAAAAGGCTGGATCGCGCTCAAGAACTTCGCCCTCAATATGAATTTCCTGATGAGCATCGCCGTCATCGGTGCG
CTTGCCATCGGCGAATGGCCGGAAGCTGCGGTGGTGATTTTCCTGTTCGCGCTGGCCGAGTTGATCGAAACGCTCTCGCT
GGAGCGGGCCAAGAACGCAATCAAGGGGCTGATGGCGATGACTCCCGAGGTCGCCACCGTGCAACTCGACAACGGCGAAT
GGCGCGAGACAGCAGTAACCGACGTGCAGGTTGGGCAAGTGGTGCGCGTCAAACCGGGAGAGCGTATTCCGCTGGACGGC
GTGGTCACGGCGGGCGGCAGTTCGGTGAACCAGGCGCCGATTACCGGGGAGAGCATGCCCGTGGTGAAGGCGGCGGGCGA
TCCGGTGTTCGCCGGTACGCTGAACGAGCGCGGTATGCTGGAGTTTCGCGTCACCGCAAACAAGGGCAACACCACGCTGG
ATCGCATCATCCACACGGTGCAAGAGGCGCAAGGCCAACGCGCGCCGACGCAACGTTTCGTCGACCAGTTCGCGCGTTAC
TACACCCCCGCCGTGGTGGTGTTCGCGGTTCTGGTGGCGGTCGTTCCTCCGTTGCTGTTCGGTGCCGCGTTCGAGCCATG
GTTCTACAAGGCGCTGGTCATGCTGGTGATCGCCTGCCCCTGCGCGCTGGTAATCTCCACGCCCATTACCGTGGTGAGCG
GGCTTGCCGCTGCGGCAAGGCAGGGCATACTGATCAAGGGCGGCGTGCATCTGGAGAACGGACGCCTGATAAAAGCCGTA
GCTTTGGACAAGACCGGCACGCTCACGCACGGCAAGCCTGTCGTGACCGATGTGGTTCCGCTGGTTGAGTTGCCTGCCGA
CAGCCTGCTGCAACTGGCCGCCAGCGTGGACGTGCATTCCGAACATCCCATCGCGTCTGCCATCGTATCGGCATGGCAGA
CGCCTGCCGACGGGAACGGAACGCCGCGTTCGTTGCTACCCGCCATTTCGTTCGAGTCCCTCACAGGTCGCGGTGCCAAA
GCGGTTGTCGAGGGGCAACTTTACTATGTGTGCAACCATCGGCAGATCGAAGAACTGGGCATCTGCGGAGTGCATGTCGA
AGAGGCTCTGCATCGTCTGGAGCAGGAAGGCAAGACAGCCGTGGTGCTGGCTACGGCAACCGAGTCGCTGTGCGTCATCG
GCGTGGCCGACACGGTGCGCGGCCACAGCGCGGAGGCTATCCGCCAACTTCATGCTCTCGGTGTGGCATCAGTGATGTTG
ACCGGCGACAACCAGACGACGGCCAGCGCCATCGCTGCCCAGGTGGGCATAGACGATGCGCGCGGCAATCTGCTGCCGGA
AGAAAAACTGGCAGTAATCGATGAGTTGATGAACCGTTACGATCATGTCGGCATGGTGGGTGATGGAATCAACGACGCGC
CTGCGTTAGCCAAGGCATCTATAGGATTTGCTATGGGCAGCGCCGGTACAGATACTGCCATCGAGACCGCCGATGTTGCA
CTGATGGATGACGATTTGCGCAAAGTGCCACACTTCATCCAGCTAAGCCGAAATACTTCCCGTGTGCTCAAACAAAACAT
CACGCTGGCCATCGGCATCAAGGCGATATTCTTCGCTTTGGCTCTGGCAGGGAAAGCCACGCTCTGGATGGCGGTCTTTG
CCGATATGGGGGCCAGCCTGATCGTGGTATTTAATGGGATGCGCTTGCTCCGTATTAAGGGCGGTATTGAACGCGACCAC
ATAAAATCTGGCCATACGAGATGA

Protein sequence :
MKHSMFNIRNMDCPTEEALIRKRLGSMPGIGELSFNLITRRLAVTHTLDDEQPILDALREIGMKTDPEPQCQSGSCSSCE
AESPVVSRRTWALMAVSGTAAMVAEIVAWNGASEQSVAVIALALLSIVTGGLSTLKKGWIALKNFALNMNFLMSIAVIGA
LAIGEWPEAAVVIFLFALAELIETLSLERAKNAIKGLMAMTPEVATVQLDNGEWRETAVTDVQVGQVVRVKPGERIPLDG
VVTAGGSSVNQAPITGESMPVVKAAGDPVFAGTLNERGMLEFRVTANKGNTTLDRIIHTVQEAQGQRAPTQRFVDQFARY
YTPAVVVFAVLVAVVPPLLFGAAFEPWFYKALVMLVIACPCALVISTPITVVSGLAAAARQGILIKGGVHLENGRLIKAV
ALDKTGTLTHGKPVVTDVVPLVELPADSLLQLAASVDVHSEHPIASAIVSAWQTPADGNGTPRSLLPAISFESLTGRGAK
AVVEGQLYYVCNHRQIEELGICGVHVEEALHRLEQEGKTAVVLATATESLCVIGVADTVRGHSAEAIRQLHALGVASVML
TGDNQTTASAIAAQVGIDDARGNLLPEEKLAVIDELMNRYDHVGMVGDGINDAPALAKASIGFAMGSAGTDTAIETADVA
LMDDDLRKVPHFIQLSRNTSRVLKQNITLAIGIKAIFFALALAGKATLWMAVFADMGASLIVVFNGMRLLRIKGGIERDH
IKSGHTR

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ORF C97 AAN62190.1 putative metal-transporting P-type ATPase Not tested PAGI-2(C) Protein 2e-176 59
SE0075 NP_763630.1 cadmium resistance protein B Not tested SCCpbp4 Protein 4e-124 48
cadA BAC57487.1 Probable cadmium-transporting ATPase (Cadmium efflux ATPase) Not tested Type-IIIinv SCCmec Protein 1e-122 43
unnamed BAB47609.1 cadmium resistance protein B Not tested Type-III SCCmec Protein 9e-123 43

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Galf_2176 YP_003847945.1 cadmium-translocating P-type ATPase BAC0298 Protein 5e-175 58
Galf_2176 YP_003847945.1 cadmium-translocating P-type ATPase BAC0461 Protein 2e-85 44
Galf_2176 YP_003847945.1 cadmium-translocating P-type ATPase BAC0092 Protein 4e-83 42
Galf_2176 YP_003847945.1 cadmium-translocating P-type ATPase BAC0124 Protein 2e-74 42