Gene Information

Name : Clocel_0389 (Clocel_0389)
Accession : YP_003841933.1
Strain : Clostridium cellulovorans 743B
Genome accession: NC_014393
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : -
Position : 497477 - 499237 bp
Length : 1761 bp
Strand : +
Note : KEGG: cbi:CLJ_B3675 pyruvate kinase; TIGRFAM: pyruvate kinase; PFAM: Pyruvate kinase barrel; Pyruvate kinase alpha/beta; PEP-utilising protein mobile region

DNA sequence :
ATGCAAAAAACTAAAATGGTATTTACAATTGGTCCTGCTAGCGAAACAGAAGAGGTTTTATCAGGTTTAATAAACGCTGG
TATGAGCTGTTCAAGACATAACTTTTCTCATGGTGACCATGCAGAACACAAAGGTAGAATTGATCTTGTTAAAGCTTTAA
GAACAAAATTCAATAAGCAAATAGCAATAATGCTTGATACAAAAGGACCAGAAATAAGAACTGGCAAATTTGATCCTAAA
AAGGTTGAATTACAAAAAGGTGACAAATTCACAATACACTGTGGAGATATGGATTTAATAGGTGATACAACTAAATGTGC
AATCACTTATACTGAACTTTACAAAGATGTTGTAAAAGGAAATACAATCCTTATCGATGACGGTTTAGTAGGACTAACTG
TTGAAGAAATAGAAGGAAACAAAATCCATTGTACAGTACAAAACACTGGATTCGTTGCAACTCATAAAGGTGTTAACGTA
CCAAATGTTTCAATCAAATTACCAGCAATGACTGATAAAGATAGAGCAGATTTAGTTTTTGGTTGCGAACAAGGAGTAGA
TTTAATTGCTGCTTCATTCATAAGAAAAGCTGCTGACGTATTAGCTATTAGAAAAGTTCTTGAAGAAAACGGTGGAGCAG
ATATCCAAATCTTCTCAAAAATCGAAAATCAAGAAGGTGTAGATAACATAGATGAAATCCTTGAATTCTCAGACGGTATC
ATGGTTGCTAGAGGAGACATGGGTGTTGAAATTCCAATAGAAAATGTTCCAGTTGTTCAAAAAATGATTATCGAAAAGTG
TAACAAAGCTGGAAAACCAGTTATAACAGCTACTCAAATGCTTGATTCTATGATGAGAAATCCAAGACCAACAAGAGCAG
AAGCTTCTGACGTTGCAAATGCTATATTTGATGGTACTGATGCAGTAATGTTATCAGGAGAATCAGCTAATGGTGATTAT
CCAATAGTTGCTGCTGAAACTATGGCTAAAATCTGTCAAAATGCTGAAAAGCACATCGACTATGCTAAAGCTCTTGAAAA
GAGAAAAGAACAATCAATTCCTAATGTTGCAAACGCTATAAGCTTAGCTACTTGCACAACTGCTATGGAATTAAATGCTG
CTGCTATAATCACAGCTACTCAAAGCGGTTACACAGCTAAAGCTGTTTCAAAATATAGACCAGCTAGCCCAGTTATAGCT
GTAACTCCAAATGAAAAAGCTGCTAGAAAGCTTGCTTTAAACTTTGGTGTTATCGCACTAGTTTCAAACAAAGTTGAAAC
TACAGATGAATTAATTAGTGATGCAGTTGCAAAAGCAAAAGAAACTGAATTAGTTAACGATGGAGATTTAGTAGTAATAG
CTGCTGGACTTCCAGTACACGTATCAGGAACTACTAACATGTTAAAAGTACATGTTGTTGGTGACGTACTAGTATCAGGA
AAAGGTGTTGGTAAAGCTGCTGGCTTTGGTAATGCAAAAGTTATAACAAATGTAGCTGATGCTGCTAAATTAGTTCAAAG
AGACGATGTTATTGTTGTTAAGAACTTAGACAAAAACTTAATGGAAGTTTTAGATAAAGTTGCTGGAGTAATTGCAGAAG
AAGGCGGAGTTACTTCACACATGGCTATTGAATGTGTATCAAGAAATATTCCTTTAATCTGCAATGCTGGTGGAGCAACA
GCTGCTATCAAAGATGGCACATTTGTTACTTTAGACGTACAAAGAGGCGTTGTTTACGAAGGTAAAGCTAACGTTAAATA
G

Protein sequence :
MQKTKMVFTIGPASETEEVLSGLINAGMSCSRHNFSHGDHAEHKGRIDLVKALRTKFNKQIAIMLDTKGPEIRTGKFDPK
KVELQKGDKFTIHCGDMDLIGDTTKCAITYTELYKDVVKGNTILIDDGLVGLTVEEIEGNKIHCTVQNTGFVATHKGVNV
PNVSIKLPAMTDKDRADLVFGCEQGVDLIAASFIRKAADVLAIRKVLEENGGADIQIFSKIENQEGVDNIDEILEFSDGI
MVARGDMGVEIPIENVPVVQKMIIEKCNKAGKPVITATQMLDSMMRNPRPTRAEASDVANAIFDGTDAVMLSGESANGDY
PIVAAETMAKICQNAEKHIDYAKALEKRKEQSIPNVANAISLATCTTAMELNAAAIITATQSGYTAKAVSKYRPASPVIA
VTPNEKAARKLALNFGVIALVSNKVETTDELISDAVAKAKETELVNDGDLVVIAAGLPVHVSGTTNMLKVHVVGDVLVSG
KGVGKAAGFGNAKVITNVADAAKLVQRDDVIVVKNLDKNLMEVLDKVAGVIAEEGGVTSHMAIECVSRNIPLICNAGGAT
AAIKDGTFVTLDVQRGVVYEGKANVK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 3e-105 52
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 3e-104 52
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 3e-105 52
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 3e-105 52

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Clocel_0389 YP_003841933.1 pyruvate kinase VFG0479 Protein 1e-105 52