Gene Information

Name : COB47_1809 (COB47_1809)
Accession : YP_003841071.1
Strain : Caldicellulosiruptor obsidiansis OB47
Genome accession: NC_014392
Putative virulence/resistance : Resistance
Product : D-alanine--D-alanine ligase
Function : -
COG functional category : M : Cell wall/membrane/envelope biogenesis
COG ID : COG1181
EC number : 6.3.2.4
Position : 1947668 - 1948762 bp
Length : 1095 bp
Strand : -
Note : TIGRFAM: D-alanine/D-alanine ligase; KEGG: ate:Athe_2039 D-alanine/D-alanine ligase; PFAM: D-alanine--D-alanine ligase domain protein

DNA sequence :
ATGACCAAGTTAAAAGTTGCTGTTTTGTTTGGAGGAGTTTCAACAGAGCACGAAATATCCATAGTTTCGGCAAAATCAAT
TATGCAAAATATGGACAAGGAAAAATACGAAATAATTCCAATAGGTATAACAAAAGAAGGAAAATGGCTCTTGTACACAG
GCAAAATTGAAGATTTGGATAGCAAATGGACTCAGTTTTCTATAGAATGTTTTGTCTCTCCTGATAGAACCAAAAAGGCA
CTTGTAAAAATAAAAGATAACGAGGCTACCTTTATCGACATTGACGTGGTTTTTCCGGTTTTGCATGGACTGAATGGTGA
GGACGGGACAGTTCAGGGACTTTTAGAGTTATCTAACATACCGTATGTGGGCTGCGGTGTTCTTTCGTCGGCTATATGTA
TGGACAAAGCATTTGCTAAAAAGCTTGCGCTTTTAGAAGGAATACCACAGGGGCATTTTTTAGTTGTATACAAAAACGAA
TATTCAGCTAAAAAAGATTATTTCATAAGAAGAATAGAGAGTGAGTTTTCGTATCCTGTTTTTGTAAAGCCTGCAAACTC
AGGCTCGTCTGTGGGTATATCAAAAGCGAAAGATAGAGAAGACCTTGTTTTGGCGATACATGAGGCTTTTTTGTATGATA
CAAAGGTTTTGATTGAACAGGCTATAAACGCTCGGGAGATAGAATGTGCAGTTTTGGGAAATGATGAGGTATTTGTGTCT
GAGCCGGGTGAAATAATTCCATCAAGAGAGTTTTATTCGTATGAAGCAAAATATATTGATGATTCATCAGAGCTCATCAT
CCCGGCAAGACTTCCAAAAGAAGTTACTGAAGAGATAAAAGATTTGGCAGCAAGGATTTACAAAATTTTTGAGTGCTGTG
GAATGGCAAGAGTGGACTTTTTTGTTGATAAAGATACGAACAAAGTGTATTTCAACGAGGTAAACACAATACCTGGTTTT
ACAAGTATTTCGATGTATCCAAAACTTATGGAGTTCAGTGGTATTCCATATTCTCAGCTCATTGATAAACTAATTTCTCT
TGCCATTGAGAAGAATGAGCAGAAAAAAAGCATAAAATACAGCAAAGAGGGCTGA

Protein sequence :
MTKLKVAVLFGGVSTEHEISIVSAKSIMQNMDKEKYEIIPIGITKEGKWLLYTGKIEDLDSKWTQFSIECFVSPDRTKKA
LVKIKDNEATFIDIDVVFPVLHGLNGEDGTVQGLLELSNIPYVGCGVLSSAICMDKAFAKKLALLEGIPQGHFLVVYKNE
YSAKKDYFIRRIESEFSYPVFVKPANSGSSVGISKAKDREDLVLAIHEAFLYDTKVLIEQAINAREIECAVLGNDEVFVS
EPGEIIPSREFYSYEAKYIDDSSELIIPARLPKEVTEEIKDLAARIYKIFECCGMARVDFFVDKDTNKVYFNEVNTIPGF
TSISMYPKLMEFSGIPYSQLIDKLISLAIEKNEQKKSIKYSKEG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
vanB NP_815951.1 D-alanine--D-lactate ligase Not tested Not named Protein 1e-59 43
vanB YP_008394259.1 Vancomycin B-type resistance protein vanB, D-alanine--D-lactate ligase Not tested Not named Protein 6e-59 42

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
COB47_1809 YP_003841071.1 D-alanine--D-alanine ligase NC_014475.1.orf3.gen Protein 1e-60 43
COB47_1809 YP_003841071.1 D-alanine--D-alanine ligase NC_005054.2598280.p0 Protein 1e-60 43
COB47_1809 YP_003841071.1 D-alanine--D-alanine ligase AF130997.1.orf4.gene Protein 3e-58 43
COB47_1809 YP_003841071.1 D-alanine--D-alanine ligase U35369.1.gene6.p01 Protein 6e-60 43
COB47_1809 YP_003841071.1 D-alanine--D-alanine ligase AE016830.1.gene2250. Protein 6e-60 43
COB47_1809 YP_003841071.1 D-alanine--D-alanine ligase AF310956.2.orf5.gene Protein 9e-60 43
COB47_1809 YP_003841071.1 D-alanine--D-alanine ligase U00456.gene.p01 Protein 6e-60 43
COB47_1809 YP_003841071.1 D-alanine--D-alanine ligase AF277571.gene.p01 Protein 3e-58 42
COB47_1809 YP_003841071.1 D-alanine--D-alanine ligase AY145441.gene.p01 Protein 9e-60 42
COB47_1809 YP_003841071.1 D-alanine--D-alanine ligase AY489045.gene.p01 Protein 4e-56 42