Gene Information

Name : Closa_0670 (Closa_0670)
Accession : YP_003820921.1
Strain : Clostridium saccharolyticum WM1
Genome accession: NC_014376
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 757349 - 758785 bp
Length : 1437 bp
Strand : +
Note : TIGRFAM: pyruvate kinase; KEGG: eel:EUBELI_01133 pyruvate kinase; PFAM: Pyruvate kinase barrel; Pyruvate kinase alpha/beta

DNA sequence :
ATGAAGAGAACCAAAATTATTTGCACCATGGGACCGAATACCAATGATCGCGCACTGATGAAGGCACTTGCCCAGAATGG
GATGGATGTGGCCAGATTCAACTTCTCACATGGTGATTATGAAGAGCAGAAAATGCGTCTCGGCTTACTAAAGAGCATCC
GCGAGGAACTGGATCTTCCCATTGCCGCACTTCTTGATACAAAAGGACCTGAGATCCGTACCGGACTTTTAAAGGATGGG
AAAAAGGTGAACCTGAAGGAAGGAGAGACATATATCCTGACAACAGAGGAGATCGTAGGAGATGCAAAAAAGGGCCACAT
CAACTATGACGGACTGAATGAGGATGTTGCTTCAGGAAACCGCATTCTTATTGATGATGGCCTGATCGAACTGGAAGTCC
TTGAGGTTAAGGGGAAGGAAATTGTATGTAAGATCATCAATGGCGGTGAGCTGGGGGAAAGAAAAGGCGTCAACGTGCCA
AATGTCAAGGTAAAGCTTCCTGCGCTTACCGATAAGGATAAAATGGATATAAAGTTCGGCATTGAGCAGGGCTTTGATTT
CATTGCGGCTTCCTTTGTACGGACAGCAGATGCAATTTACGAAATAAAGAATATATTGGAGGAGCATGGTTCCAATATAT
CCGTTATTGCCAAGATCGAGAATGCGGAGGGCATTGAAAATCTAGATGACATCATTGAAGCCAGCGATGGAATCATGGTT
GCCCGGGGCGATATGGGAGTTGAGATTCCGGCCCAGGAGGTACCGTTTATCCAGAAGCGGATTATAGAGAAATGCAACGA
GGCCTGTAAGCCTGTTATAATCGCAACCCAGATGCTGGATTCCATGATCCGCAATCCCCGTCCTACCAGAGCCGAGGTAA
CGGACGTGGCAAACGCTGTTTATGACGGGACGGATGCAGTCATGCTTTCCGGTGAGACCGCTATGGGCAAATATCCGGTT
GAGGCTCTTTCCATGATGGCCTCGATTGTGGAGGAAACAGAAAAGCATCTGGATTACAGTGCATACAGAGAGCGCAAGGT
TTCTGCCGTCAATAGCCACAATGTATCCAATGCAGTATGCTATTCTTCCGTTTCCACGGCTCATGATCTGGGAGCCAGAG
TGATCGTGGCTCCAAGCATCACCGGCTTTACTACCCGGCTGTTGTCCAAATGGAGACCGGAAAGCCTGATCATCGGCTTG
TCCCCAAGCTCCTCCGCCCTCCGTCAGATGCAGTTATACTGGGGCGTAAAGCCGTTCCATGCGAAGCGTGCGGAATCCAC
CGACGTTTTGATTTATTCTTCCATGGAATTATTAAAGGCAAAAGGCATTGTAAAAGAAAATGAAATGGTAGTGGTGACTG
CTGGGGTGGTAAGTCCTGTGAGAAAGAACGAACCTGCGGCTCATACCAATATCATGCGGGTGGTGACCGTAGATTAA

Protein sequence :
MKRTKIICTMGPNTNDRALMKALAQNGMDVARFNFSHGDYEEQKMRLGLLKSIREELDLPIAALLDTKGPEIRTGLLKDG
KKVNLKEGETYILTTEEIVGDAKKGHINYDGLNEDVASGNRILIDDGLIELEVLEVKGKEIVCKIINGGELGERKGVNVP
NVKVKLPALTDKDKMDIKFGIEQGFDFIAASFVRTADAIYEIKNILEEHGSNISVIAKIENAEGIENLDDIIEASDGIMV
ARGDMGVEIPAQEVPFIQKRIIEKCNEACKPVIIATQMLDSMIRNPRPTRAEVTDVANAVYDGTDAVMLSGETAMGKYPV
EALSMMASIVEETEKHLDYSAYRERKVSAVNSHNVSNAVCYSSVSTAHDLGARVIVAPSITGFTTRLLSKWRPESLIIGL
SPSSSALRQMQLYWGVKPFHAKRAESTDVLIYSSMELLKAKGIVKENEMVVVTAGVVSPVRKNEPAAHTNIMRVVTVD

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 1e-99 48
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 5e-100 48
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 5e-100 48
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 2e-99 48

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Closa_0670 YP_003820921.1 pyruvate kinase VFG0479 Protein 2e-100 48