Gene Information

Name : Closa_0443 (Closa_0443)
Accession : YP_003820703.1
Strain : Clostridium saccharolyticum WM1
Genome accession: NC_014376
Putative virulence/resistance : Virulence
Product : ATP-dependent chaperone ClpB
Function : -
COG functional category : O : Posttranslational modification, protein turnover, chaperones
COG ID : COG0542
EC number : -
Position : 501298 - 503886 bp
Length : 2589 bp
Strand : +
Note : TIGRFAM: ATP-dependent chaperone ClpB; PFAM: ATPase AAA-2 domain protein; ATPase AAA; Clp domain protein; Clp ATPase-like; KEGG: eel:EUBELI_01260 ATP-dependent Clp protease ATP-binding subunit ClpB; SMART: AAA ATPase

DNA sequence :
ATGAATATTAATAAATTTACCCAAAAGTCGATGGAAGCCGTCCAGAATTGCGAAAAGCTTGCCTATGAATACGGAAATCA
GCAAATAGAGCAGGAACATATGCTTTACAGCCTTCTTACCATGGAAGACAGCCTGATTTTAAAATTAATAACAAAGATGA
ATATACAGAAAGAGCAGTTTATCAATGAAACGGCTCAGGCAATGGAAAAGCTTCCTAAGGTCAGGGGCGGGCAGCTATAC
ATCAGCAATGATTTAAATAAGGTCTTAATAAGCGCCGAGGATGAGGCAAAGGTCATGGGGGATGAATACGTTTCCGTAGA
ACATCTGTTTCTTGCCATGTTAAAGACGCCGTCAAAATCCGTAAAGGAACTGTTCCGGAACTACGGCATAACCAGAGAAA
CGTTTTTACAGGCCCTTTCCACCGTAAGAGGAAACCAGCGGGTGGTCAATGACAATCCGGAAGCTACCTATGATACCCTT
ACCAAATACGGCTTTGATATGGTAGAACGGGCCAGGGACCAGAAGCTGGATCCGGTCATCGGACGGGACAGTGAGATCCG
CAATGTTATCCGGATTCTTTCCAGAAAGACCAAGAACAACCCGGTTCTCATCGGGGAACCGGGAGTAGGTAAGACGGCAG
TGGTGGAAGGCCTGGCCCAGCGGATCGTCCGTGGCGATGTGCCTGAGGGCTTAAAGGATAAAAGGCTGTTTGCCCTTGAT
ATGGGAGCACTGCTGGCAGGTGCCAAATACCGGGGGGAATTTGAGGAACGTTTAAAGGCAGTTCTTGACGAGGTGAAGAA
AAGTGACGGACAGATCATCCTGTTCATTGATGAACTTCACACCATTGTAGGCGCTGGGAAAACAGAAGGTTCCATGGACG
CAGGCAATATGCTAAAGCCAATGCTTGCCAGAGGAGAGCTGCACTGCATCGGCGCCACAACTCTTGACGAATACCGCCAG
TATATTGAAAAGGATCAGGCCCTGGAACGGCGGTTCCAGCCGGTCATGGTGAATGAACCCAGTGTGGAAGATACCATATC
CATATTAAGAGGGCTAAAAGAGCGGTATGAGGTCTATCATGGGGTAAAGATCACGGACTCAGCCCTGGTATCGGCGGCAG
CATTATCTGACCGCTACATCAGCGAGCGTTTTCTTCCGGATAAGGCCATTGACCTGGTGGATGAAGCCTGTGCCATGATA
AAAACAGAATTGGATTCCATGCCGGCAGAGCTGGATGAGCTGTCAAGACGCATCATGCAGATGGAGATTGAAGAAGCTGC
CTTAAAGAAGGAAACCGACCGTTTGAGCCAGGACCGCCTGGCAGAGCTTCAGAAGGAGCTGGCAGAGCTTCATGACGAGT
TTACTTCTCAAAAGGCCCAATGGGAAAATGAAAAAGCTTCTGTTGACCGGTTGTCCTCCTTAAGGGAAGAAATCGAAACC
ATAAACAGGGAGATTCAGGCGGCACAGCAAAAGTATGATTTAAACCGGGCGGCGGAGCTGCAGTATGGAAAGCTTCCACA
GCTTCAAAAGGAGCTGGAGGCGGAAGAGGAACGGGTGAGAAACCAGGATTTGAGCCTTGTCCATGAAAGCGTCACAGAGG
AGGAGATCTCCAGGATTGTGTCCAAATGGACGGGAATCCCCGTATCCAGGCTGACGGAAGGGGAGAGAAATAAAACCCTT
CACCTGGATCAAGAGCTTCATAAGAGAGTCATCGGCCAGGATGAGGCAGTATCAAAGGTTACGGAGGCCATCATCCGCTC
CAAAGCAGGAATCAAGGATCCCACAAAGCCCATTGGCTCCTTCCTGTTTTTAGGACCTACCGGTGTTGGCAAGACGGAGC
TTGCCAAGGCCCTGGCAGAAAGCCTGTTTGATGACGAAAATAACATTGTCAGGATCGATATGAGCGAATATATGGAAAAG
CATTCCGTATCCAGACTGATCGGAGCGCCTCCAGGCTATGTGGGCTATGACGAAGGTGGCCAGCTGACAGAGGCAGTCAG
GAGAAAGCCCTATTCCGTAGTGCTTTTTGATGAGGTGGAAAAGGCCCATCCAGACGTATTTAACGTATTGCTTCAGGTTC
TTGATGATGGCCGGATCACAGATTCCACCGGAAAAACCGTTGACTTTAAAAACACCATCATCATCATGACCTCCAATATC
GGCTCCCAGTATCTGCTGGCAGGCATTGATGAAGAAGGATATATTAAGGAAGATGCACAGGCAATGGTAATGAACGACTT
AAGGAACCATTTCCGTCCCGAGTTCTTAAACCGTCTGGACGAAATGATCCTCTTTAAGCCTCTTGACAAGGAAAATATTG
CAGGTATCATAGATCTGCTCCTTGCAGATTTAAATAAACGGGTGGAAAATCAGGAATTAAAGATTGAACTGACTGACCAG
GCCAAGGAATTTGTGGTGGAACAGGGATATGATCCGGTTTACGGAGCCCGTCCCCTGAAACGTTACTTGCAAAAGCATGT
GGAAACCTTAGCAGCCAGGATCATCCTTGGGGATGAGGTTCGGGCAGGAAATGTGATCGTCATTGATGTATCGGAAGACG
GCCAGAAGCTGATCGCCTATCTGGAGTAA

Protein sequence :
MNINKFTQKSMEAVQNCEKLAYEYGNQQIEQEHMLYSLLTMEDSLILKLITKMNIQKEQFINETAQAMEKLPKVRGGQLY
ISNDLNKVLISAEDEAKVMGDEYVSVEHLFLAMLKTPSKSVKELFRNYGITRETFLQALSTVRGNQRVVNDNPEATYDTL
TKYGFDMVERARDQKLDPVIGRDSEIRNVIRILSRKTKNNPVLIGEPGVGKTAVVEGLAQRIVRGDVPEGLKDKRLFALD
MGALLAGAKYRGEFEERLKAVLDEVKKSDGQIILFIDELHTIVGAGKTEGSMDAGNMLKPMLARGELHCIGATTLDEYRQ
YIEKDQALERRFQPVMVNEPSVEDTISILRGLKERYEVYHGVKITDSALVSAAALSDRYISERFLPDKAIDLVDEACAMI
KTELDSMPAELDELSRRIMQMEIEEAALKKETDRLSQDRLAELQKELAELHDEFTSQKAQWENEKASVDRLSSLREEIET
INREIQAAQQKYDLNRAAELQYGKLPQLQKELEAEEERVRNQDLSLVHESVTEEEISRIVSKWTGIPVSRLTEGERNKTL
HLDQELHKRVIGQDEAVSKVTEAIIRSKAGIKDPTKPIGSFLFLGPTGVGKTELAKALAESLFDDENNIVRIDMSEYMEK
HSVSRLIGAPPGYVGYDEGGQLTEAVRRKPYSVVLFDEVEKAHPDVFNVLLQVLDDGRITDSTGKTVDFKNTIIIMTSNI
GSQYLLAGIDEEGYIKEDAQAMVMNDLRNHFRPEFLNRLDEMILFKPLDKENIAGIIDLLLADLNKRVENQELKIELTDQ
AKEFVVEQGYDPVYGARPLKRYLQKHVETLAARIILGDEVRAGNVIVIDVSEDGQKLIAYLE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY0294 NP_454876.1 ClpB-like protein Not tested SPI-6 Protein 8e-108 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Closa_0443 YP_003820703.1 ATP-dependent chaperone ClpB VFG2084 Protein 2e-110 42