Gene Information

Name : Closa_3466 (Closa_3466)
Accession : YP_003823615.1
Strain : Clostridium saccharolyticum WM1
Genome accession: NC_014376
Putative virulence/resistance : Virulence
Product : FliI/YscN family ATPase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : -
Position : 3731231 - 3732532 bp
Length : 1302 bp
Strand : -
Note : TIGRFAM: ATPase, FliI/YscN family; PFAM: H+transporting two-sector ATPase alpha/beta subunit central region; KEGG: cpy:Cphy_2714 flagellar protein export ATPase FliI; SMART: AAA ATPase

DNA sequence :
ATGTTTAAAGAACTGTCAGATCTCATCATGAAAACTGAGACCATTGATCATATTGGAAAGATTGAAAATATTGTGGGGAT
GTCGATGGAAGCCTCCGGCGGAAAGGCCAGTATCGGGGACATTGCCTATATTTATAACGAAGACAGGAAAGAGCAGATTC
CGGTGGAAGTAGTGGGCTTTAAAGATGATAAAATACAGCTTATGGCCTATGAAAATATGAATGGGATTGCTGCCGGCAGC
TTTGTACGCAATACGAAACGCCGATTGAAGATTCCGGTAGGAGATTTTTTAAGAGGCCGGATCATCGATGCAAAGGGAGA
ACCCATGGATGGAAAGGGTCCCTTTGATTCTTCCCGGTATTATTATGTGGAAAATCCCTATATTAACCCATTGACCCGTC
CCCCCATTACGGACAGGCTGGAGTTTGGAGTCAAGGCCATCGATGGTATGAATACCATCGGAAAAGGACAGCGTATCGGA
ATCTTTGCGGGAAGCGGTGTGGGGAAGAGTACCTTGCTGGGAATGATTGCAAGAAATGTCAAGGCTGACATTAATGTGGT
AGCGCTTGTAGGCGAGCGAGGACGAGAGGTCAGGGAATTTATAGAAAAGGATTTAGGACCGGAAGGGATGGAACGTTCTG
TCCTGGTGGTGGCTACATCGGACCAGCCGGCCATGCTTCGCATGAAGTGCCCCCTGGTAGCAACCACCATAGCAGAATAT
TTTAAGAACCAGGGCAAGGATGTGCTTTTGATGATGGACTCTCTGACTCGTTTTGCCATGGCACAGAGAGAAATAGGACT
TGCGATAGGCGAGCCGCCGGTGGCAAGGGGCTATACTCCCTCCATTTATGCGGAGTTTCCCAAGCTTCTGGAACGAAGCG
GTAATTTTGGTGAAGGTTCCATTACCGGAATTTATACGGTTCTCGTGGAGGGCGATGATACCAATGAACCCATTGCAGAT
ACCGTAAGAGGTATTGTGGATGGGCACATCGTTTTAAGCAGAAAACTGGCAAATGCCAATCATTTCCCAGCCATTGATGT
AAGTGCCAGCATATCCCGTCTGATGACGAACATCGTGTCTGAGGAGCATCGAAGGCTGGCATCGGAAATCAGGGACATTT
TAAGCATTTATGAAAAAAATGAGGATTTGATTTCCATCGGCGCATACAAAAGCGGTACGAACCCCAAGCTGGATCAGGCC
ATTGCCAGAATCGACAAGGTAAACCAGTTCCTGTGCCAGGGAATCCAGGAAAACGACAGCTATGAAGATATACTGGTTAA
GATGAAAGCAATCTTAGAATAA

Protein sequence :
MFKELSDLIMKTETIDHIGKIENIVGMSMEASGGKASIGDIAYIYNEDRKEQIPVEVVGFKDDKIQLMAYENMNGIAAGS
FVRNTKRRLKIPVGDFLRGRIIDAKGEPMDGKGPFDSSRYYYVENPYINPLTRPPITDRLEFGVKAIDGMNTIGKGQRIG
IFAGSGVGKSTLLGMIARNVKADINVVALVGERGREVREFIEKDLGPEGMERSVLVVATSDQPAMLRMKCPLVATTIAEY
FKNQGKDVLLMMDSLTRFAMAQREIGLAIGEPPVARGYTPSIYAEFPKLLERSGNFGEGSITGIYTVLVEGDDTNEPIAD
TVRGIVDGHIVLSRKLANANHFPAIDVSASISRLMTNIVSEEHRRLASEIRDILSIYEKNEDLISIGAYKSGTNPKLDQA
IARIDKVNQFLCQGIQENDSYEDILVKMKAILE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
lscN AAO18044.1 LscN Virulence TTSS locus Protein 1e-91 50
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 8e-83 50
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 3e-85 49
escN AAC38383.1 EscN Virulence LEE Protein 2e-85 49
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 1e-84 49
escN NP_290268.1 hypothetical protein Virulence LEE Protein 4e-85 49
ECs4568 NP_312595.1 EscN Virulence LEE Protein 4e-85 49
escN YP_003236088.1 translocator EscN Virulence LEE Protein 1e-85 49
escN AAC31513.1 L0034 Virulence LEE Protein 3e-85 49
escN YP_003223475.1 translocator EscN Virulence LEE Protein 2e-84 48
escN AAK26715.1 EscN Virulence LEE Protein 2e-84 48
escN AAL57542.1 EscN Virulence LEE Protein 2e-84 48
escN CAC81862.1 EscN protein Virulence LEE II Protein 1e-84 48
escN CAI43874.1 EscN protein Virulence LEE Protein 1e-84 48
unnamed AAL06369.1 EscN Virulence LEE Protein 1e-82 47
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-80 47
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-80 47
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-90 47
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-91 47
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-93 47
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 3e-90 47
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-93 47
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 2e-91 47
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 2e-91 47
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 2e-91 47
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-90 47
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-80 46
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 1e-79 46
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 1e-79 46
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 1e-80 46
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-80 45
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 2e-66 45
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 8e-66 44
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 6e-71 44
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 9e-68 44
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 9e-68 44
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 3e-71 43
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 2e-71 43
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 2e-71 43
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 3e-67 43
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 2e-76 42
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 4e-64 42
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 4e-64 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Closa_3466 YP_003823615.1 FliI/YscN family ATPase VFG2144 Protein 5e-97 51
Closa_3466 YP_003823615.1 FliI/YscN family ATPase VFG0048 Protein 1e-94 49
Closa_3466 YP_003823615.1 FliI/YscN family ATPase VFG0390 Protein 1e-96 49
Closa_3466 YP_003823615.1 FliI/YscN family ATPase VFG0192 Protein 8e-96 49
Closa_3466 YP_003823615.1 FliI/YscN family ATPase VFG0730 Protein 1e-85 49
Closa_3466 YP_003823615.1 FliI/YscN family ATPase VFG0812 Protein 2e-85 49
Closa_3466 YP_003823615.1 FliI/YscN family ATPase VFG1254 Protein 2e-96 48
Closa_3466 YP_003823615.1 FliI/YscN family ATPase VFG2499 Protein 6e-90 48
Closa_3466 YP_003823615.1 FliI/YscN family ATPase VFG2331 Protein 6e-87 46
Closa_3466 YP_003823615.1 FliI/YscN family ATPase VFG0515 Protein 8e-81 46
Closa_3466 YP_003823615.1 FliI/YscN family ATPase VFG1778 Protein 1e-66 42
Closa_3466 YP_003823615.1 FliI/YscN family ATPase VFG1008 Protein 6e-61 41