Gene Information

Name : Closa_3181 (Closa_3181)
Accession : YP_003823334.1
Strain : Clostridium saccharolyticum WM1
Genome accession: NC_014376
Putative virulence/resistance : Resistance
Product : small GTP-binding protein
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG0480
EC number : -
Position : 3422427 - 3424376 bp
Length : 1950 bp
Strand : +
Note : KEGG: cdf:CD0508 conjugative transposon tetracycline resistance protein; TIGRFAM: small GTP-binding protein; PFAM: protein synthesis factor GTP-binding; elongation factor G domain IV; elongation factor G domain protein

DNA sequence : 
ATGAAAATTATGAATATAGGCATCTTAGCTCATGTAGATGCAGGAAAAACAACATTAACAGAGAGTATGCTCTATACCAG
CGGTGCTATTGCAAAATCCGGACGTGTTGATAGTGGTTCGACGATTACAGATTCTATGACACTTGAAAAGCAGAGGGGAA
TTACGATACAAGCATCCATTGCATCTTATCAATGGAATCATGTGAAAATTAATTTAATAGACACCCCGGGGCACATAGAC
TTCTATTCAGAAGTAGAGCGTTCATTGAATATACTGGATGGTGTCGTTTTGTTAATCTCAGCCAAAGACGGCATTCAGGC
ACAAACACGGATCCTTTACGATGCAATAAAGAAAAAGAAGCTTCCTGCTTTCATTTTTATTAATAAGATTGATCAGCCCG
GTATTGATCTTGAGTTGCTTTATAAAGATATCAAAGAGAAATTAGCTTCCCACATTCTTATAATGCAGAGCTTTAAAAAT
GATTATACGCTTTCCCCGAAGCAGGTTGACCGATTGTCAGAAGAGTTTAGAGACACAATTATTGAAACGGATGATGCGTT
GCTGGAGAAATTTATCCTTGATCAGTCTATTACTCCTGAAGAACTGTTAATTAGCAGAAGAAAGAATATAATCAATGGGA
AGCTTTTACCTATTTATCATGGCAGTGCGCTTAAGCATATAGGTACAAAAGAACTAATGGATGCCCTTCTCTTAGAATTC
CAGCCTTACCCATTACCCACGGACTCCAAGCTTGCTGCATTGGTATATAAGATTGAAAGAGATGATCATCGGAATAAGCG
CACTTATATGCGTATTTTTGGAGGTTCTATAAAAGCGCGTGATGTGCTGATCCCCGAAGGGCATTCCGACAGCATAAAAA
TAAAAAAGTTAGAAACCTCCTGCAATGGCAAAATTGTAGAAACCGAAAAAATTGAATGCAGTGATATCGCAATTTTTCCC
AATGAAGCTCAATTAAAAATTGGAGACTCCATAGGAGCAATACCGAATAGCAACCTGATTTTGCACAATCATAACCTGAT
CATGCAGGCCAATATTTCCCCTGTCTTTTCTTCTGACCGGGCATTTCTATTAGAAGCCTTGTCAGAACTGGCCGAAACGG
ATCCTCTTTTGCAATATAAATTGGATTCCAGAAGCAGTGATATCACAATGGAGTTTCTTGGAAAAGTGCAAATGGAGGTC
ATCGCTGCCTTATTGCAGAGCAGATACCATCTAAAAGTTAAAATAGAAAATGTAACCACCATTTATAAAGAACGGCCCTT
GAAAAAAGCAAACTTTACCGCCCATATTGAAATCCCTCCAAATCCTTTCTGGGCCTCCATTGGTTTATCAATAGAACCTC
TTCCGATTGGAGCCGGATTATATTATGAAAGTAACGTGTCCTATGGGTATTTAAATAGGTCGTTCCAGAATGCGGTTGAA
GAAGGCATCCGATATGGATGTGAGCAAGGGCTGTATGGGTGGGAAGTAACCGATCTAAAAGTTATTTTTGAATATGGATT
GTACTACAGTCCCGTAAGTACGCCAGCTGATTTCCGACATTTGGCTCCGATTGTGTTTGAGCAGGCTTTGAAACAATCAG
GTACAGAGCTGTTGGAGCCATGCCTGTCATTTGAACTTTATGTTCCCCAAGACTGCAATGCCCGTGTTTACAATGACTTA
AAAAAATTTCATGCAGATATTGAGTCAATAAAAACAAAGCATCATGAAATCGTAGTGGCTGGAAAAATTCCGGCCCGCAC
ATCACAATTATACAAAGAACAGCTTTCAGAGCTCACAAAAGGCCGCGGAGTTTTTTTAACAGAACAGGCAGGGTACCAGA
AAAATACCGGAGAAATTTTTACTCAGGCCAGAAAACCGGATGACCGGTTGGATAAAACCCGCTACATGTTTGATAAGGCC
TGTATCGGCAATGAAGATACTAAAATGTAA

Protein sequence : 
MKIMNIGILAHVDAGKTTLTESMLYTSGAIAKSGRVDSGSTITDSMTLEKQRGITIQASIASYQWNHVKINLIDTPGHID
FYSEVERSLNILDGVVLLISAKDGIQAQTRILYDAIKKKKLPAFIFINKIDQPGIDLELLYKDIKEKLASHILIMQSFKN
DYTLSPKQVDRLSEEFRDTIIETDDALLEKFILDQSITPEELLISRRKNIINGKLLPIYHGSALKHIGTKELMDALLLEF
QPYPLPTDSKLAALVYKIERDDHRNKRTYMRIFGGSIKARDVLIPEGHSDSIKIKKLETSCNGKIVETEKIECSDIAIFP
NEAQLKIGDSIGAIPNSNLILHNHNLIMQANISPVFSSDRAFLLEALSELAETDPLLQYKLDSRSSDITMEFLGKVQMEV
IAALLQSRYHLKVKIENVTTIYKERPLKKANFTAHIEIPPNPFWASIGLSIEPLPIGAGLYYESNVSYGYLNRSFQNAVE
EGIRYGCEQGLYGWEVTDLKVIFEYGLYYSPVSTPADFRHLAPIVFEQALKQSGTELLEPCLSFELYVPQDCNARVYNDL
KKFHADIESIKTKHHEIVVAGKIPARTSQLYKEQLSELTKGRGVFLTEQAGYQKNTGEIFTQARKPDDRLDKTRYMFDKA
CIGNEDTKM

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
SSU98_0928 YP_001200485.1 translation elongation factor (GTPases) Not tested 89K Protein 1e-149 52
SSU05_0922 YP_001198287.1 translation elongation factor (GTPases) Not tested 89K Protein 1e-149 52
tet(W) YP_005160006.1 tetracycline resistance protein Not tested Not named Protein 2e-151 50
tetO AFJ97266.1 truncated tetracycline resistant protein Not tested Not named Protein 1e-90 46

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Closa_3181 YP_003823334.1 small GTP-binding protein AM180355.1.gene636.p Protein 8e-148 53
Closa_3181 YP_003823334.1 small GTP-binding protein M85225.gene.p01 Protein 1e-148 52
Closa_3181 YP_003823334.1 small GTP-binding protein X04388.gene.p01 Protein 3e-148 52
Closa_3181 YP_003823334.1 small GTP-binding protein FN594949.1.gene24.p0 Protein 1e-149 52
Closa_3181 YP_003823334.1 small GTP-binding protein NC_002758.1120355.p0 Protein 2e-146 51
Closa_3181 YP_003823334.1 small GTP-binding protein AY485126.gene.p01 Protein 6e-153 51
Closa_3181 YP_003823334.1 small GTP-binding protein NC_009782.5559075.p0 Protein 2e-146 51
Closa_3181 YP_003823334.1 small GTP-binding protein AJ295238.gene.p01 Protein 3e-153 51
Closa_3181 YP_003823334.1 small GTP-binding protein L09756.gene.p01 Protein 1e-145 51
Closa_3181 YP_003823334.1 small GTP-binding protein DQ294299.gene.p01 Protein 4e-151 50
Closa_3181 YP_003823334.1 small GTP-binding protein AJ222769.gene.p01 Protein 9e-151 50
Closa_3181 YP_003823334.1 small GTP-binding protein L42544.gene.p01 Protein 2e-139 48
Closa_3181 YP_003823334.1 small GTP-binding protein AJ514254.gene.p01 Protein 9e-132 45
Closa_3181 YP_003823334.1 small GTP-binding protein Z21523.gene.p01 Protein 9e-132 45
Closa_3181 YP_003823334.1 small GTP-binding protein L20800.gene.p01 Protein 4e-107 41