Gene Information

Name : Closa_1198 (Closa_1198)
Accession : YP_003821428.1
Strain : Clostridium saccharolyticum WM1
Genome accession: NC_014376
Putative virulence/resistance : Resistance
Product : serine-type D-Ala-D-Ala carboxypeptidase
Function : -
COG functional category : M : Cell wall/membrane/envelope biogenesis
COG ID : COG1876
EC number : 3.4.16.4
Position : 1294283 - 1295074 bp
Length : 792 bp
Strand : +
Note : KEGG: efa:EF2297 D-alanyl-D-alanine carboxypeptidase; PFAM: peptidase M15B and M15C DD-carboxypeptidase VanY/endolysin

DNA sequence :
ATGGTTAATTGCAATAATAATTATAGCGGCTATAAGAGGAAACGAGCATTAAAACAACATTCACGCTTGAGAAAGAACCA
TATGATATTGCGGCTGATCTCAGTAGCAGCTGTCATCGGTTTGCTTTGGTACTGCATCAATGGTTTGAATTTGAAAGAAG
TTTCGCCTGAACCTATTTTATCCATTGGGACGGACAGTCCCGACATCAGTTCAGCCGACATTAAAAAAGATGATACGGAT
GATCCTTTGTGGAGCTTGATTCTAGTCAATCGATGGAATGTGATTCCAGAGGATTACCACGTAGATTTGACCAAATTATC
AAATGGACAAACTGTTGATACACGGATATACCCGGCACTTCAGAACATGTTTGATGCAGCAAGGGCTGACGGTGTAAATC
TCGTGGTCGTTTCAGGATACCGAACGGCTGAGAAGCAGCAGAGCCTGATGGATGAAAAAGTTGCAGCATATAAAGCGGAA
GGATATTCAGCCTACCAAGCAAAGACCGAAGCAGAAACATGGGTCGCGATTCCAGGTACAAGTGAACATCAGTTGGGAAT
CGCTGTGGATATCAATGCGGATGGGGTTCAATCAAAGGGAAGTGAAGTTTATAAATGGCTGAAACAAAACGGATACCGTT
TTGGATTTATCCTCCGCTATCCGCCGGATAAAACAGAAATCACTGGTGTGAGCAATGAGCCGTGGCATTACCGCTATGTA
GGAATTAAGGCTGCCACTGAAATGAACGATCAAAACCTCTGCCTTGAGGAATATTTAAGAGATAAAAATTGA

Protein sequence :
MVNCNNNYSGYKRKRALKQHSRLRKNHMILRLISVAAVIGLLWYCINGLNLKEVSPEPILSIGTDSPDISSADIKKDDTD
DPLWSLILVNRWNVIPEDYHVDLTKLSNGQTVDTRIYPALQNMFDAARADGVNLVVVSGYRTAEKQQSLMDEKVAAYKAE
GYSAYQAKTEAETWVAIPGTSEHQLGIAVDINADGVQSKGSEVYKWLKQNGYRFGFILRYPPDKTEITGVSNEPWHYRYV
GIKAATEMNDQNLCLEEYLRDKN

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
vanYB NP_815954.1 D-alanyl-D-alanine carboxypeptidase Not tested Not named Protein 1e-63 58
vanYB YP_008394256.1 VanY protein, D-alanyl-D-alanine carboxypeptidase Not tested Not named Protein 1e-63 57

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Closa_1198 YP_003821428.1 serine-type D-Ala-D-Ala carboxypeptidase U35369.1.gene3.p01 Protein 6e-64 58
Closa_1198 YP_003821428.1 serine-type D-Ala-D-Ala carboxypeptidase AE016830.1.gene2253. Protein 6e-64 58
Closa_1198 YP_003821428.1 serine-type D-Ala-D-Ala carboxypeptidase AF310956.2.orf2.gene Protein 7e-64 57
Closa_1198 YP_003821428.1 serine-type D-Ala-D-Ala carboxypeptidase AF253562.2.orf2.gene Protein 1e-41 46
Closa_1198 YP_003821428.1 serine-type D-Ala-D-Ala carboxypeptidase DQ212986.1.gene6.p01 Protein 2e-48 45