Gene Information

Name : Olsu_1201 (Olsu_1201)
Accession : YP_003801187.1
Strain : Olsenella uli DSM 7084
Genome accession: NC_014363
Putative virulence/resistance : Resistance
Product : D-alanine--D-alanine ligase
Function : -
COG functional category : M : Cell wall/membrane/envelope biogenesis
COG ID : COG1181
EC number : 6.3.2.4
Position : 1366663 - 1367742 bp
Length : 1080 bp
Strand : +
Note : COGs: COG1181 D-alanine-D-alanine ligase and related ATP-grasp protein; InterProIPR016185:IPR000291:IPR005905:IPR011761:IPR 011127:IPR011095:IPR013817:IPR013816; KEGG: dsy:DSY3690 hypothetical protein; PFAM: D-alanine--D-alanine ligase domain protein; PRI

DNA sequence :
ATGGCAGAAAGGCTCAACGTCGCGCTCCTCTTTGGGGGGACCTCGAGCGAGCACGACGTCTCGCTGCGCTCCGCCGCCAA
CGTGATCGACCTCCTCTCGGAACGCGTCGACCTGACGCTCGTCGGTATCACGAGGGGTGGCAGCTGGCTGCTCTATGGGG
GTGACGCCACCGCCATCGCGGACGACTCCTGGCAGGGTCATGACACCACGCCGGTCTGCGCCGTGCCCGGCGATGGGGGT
GGGCTTTTCGCAGTCGAGGGTGGGTCGCCCGTCCGGCTGCCCATCGACGTGGTCTTCCCCGTACTCCACGGACAGGGCGG
GGAAGACGGCGTGACGCAGGGGCTCCTGGAGTCGTGCCGACTACCCTACGTGGGCTGTGGCGTACTCAGCTCGGCCCTCT
GCACGGACAAGGACGCCGCCCACCGCATAGTCGCTGCCGCAGGCATCCGCTCACCTCGCTGCGAGGTCGTGCACCCCGGC
GACGGGGCCACACGCATCGCCGAGGTCGCGGCCGCCTGCGGCTACCCCCTCTTCGTGAAGCCGGCCCGCGGGGGATCCAG
CTACGGCGTCAGCAAGGTCGAGGGTCCCGAGCAGCTGGACGAGGCCCTCGGGCTTGCCTTCGGCTATGACGAAAAGGTCG
CCGTCGAGGAGGCCATCGTGGGCACCGAGGTGGGCTGTGCCATCATGGGCGGCGCCGCGGGCGAGCTCGCGATGGGGCTG
GTGGACGAGACGGTCGTCTCGGATGGAGGCTTCTTCCATATCCACCAGGACCACAGCGAGGGTGCGGACGCATCGACGAG
AAACTCCTCGACTCGCTGTCCCGCGCTCCTCCCGCCCGAGGTCATGGAGCGCGTGCGCGAGACGGGAACGGCCGTCTGGC
GCGCGCTGGGCTGCTCGGGACTCGCCCGCGTCGACCTCTTCGTGACCGAGAGGGGCGAGGTGGTCTTCAACGAGGTCAAC
ACCATGCCCGGCCTTACCAGCTACTCGCGCTTCCCGGCCATGATGGCCGCAGCGGGTGCCGACATGGGGGAGGTCCTGGA
GCGCCTCGTCCGCAGCGCCGCACGGACGGGCGCCCGCTAG

Protein sequence :
MAERLNVALLFGGTSSEHDVSLRSAANVIDLLSERVDLTLVGITRGGSWLLYGGDATAIADDSWQGHDTTPVCAVPGDGG
GLFAVEGGSPVRLPIDVVFPVLHGQGGEDGVTQGLLESCRLPYVGCGVLSSALCTDKDAAHRIVAAAGIRSPRCEVVHPG
DGATRIAEVAAACGYPLFVKPARGGSSYGVSKVEGPEQLDEALGLAFGYDEKVAVEEAIVGTEVGCAIMGGAAGELAMGL
VDETVVSDGGFFHIHQDHSEGADASTRNSSTRCPALLPPEVMERVRETGTAVWRALGCSGLARVDLFVTERGEVVFNEVN
TMPGLTSYSRFPAMMAAAGADMGEVLERLVRSAARTGAR

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
vanB YP_008394259.1 Vancomycin B-type resistance protein vanB, D-alanine--D-lactate ligase Not tested Not named Protein 8e-51 43
vanB NP_815951.1 D-alanine--D-lactate ligase Not tested Not named Protein 6e-50 42

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Olsu_1201 YP_003801187.1 D-alanine--D-alanine ligase AY489045.gene.p01 Protein 6e-53 45
Olsu_1201 YP_003801187.1 D-alanine--D-alanine ligase AF277571.gene.p01 Protein 5e-54 45
Olsu_1201 YP_003801187.1 D-alanine--D-alanine ligase AF130997.1.orf4.gene Protein 8e-53 44
Olsu_1201 YP_003801187.1 D-alanine--D-alanine ligase AY145441.gene.p01 Protein 6e-51 43
Olsu_1201 YP_003801187.1 D-alanine--D-alanine ligase AF310956.2.orf5.gene Protein 3e-50 43
Olsu_1201 YP_003801187.1 D-alanine--D-alanine ligase U35369.1.gene6.p01 Protein 3e-50 42
Olsu_1201 YP_003801187.1 D-alanine--D-alanine ligase AE016830.1.gene2250. Protein 3e-50 42
Olsu_1201 YP_003801187.1 D-alanine--D-alanine ligase U00456.gene.p01 Protein 3e-50 42