Gene Information

Name : pykF (NIDE2807)
Accession : YP_003798437.1
Strain : Candidatus Nitrospira defluvii
Genome accession: NC_014355
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 2746575 - 2748011 bp
Length : 1437 bp
Strand : +
Note : Evidence 2a : Function of homologous gene experimentally demonstrated in an other organism; PubMedId : 10751408, 12802531, 1447787, 1859631, 6354749, 8436141, 8591049; Product type e : enzyme

DNA sequence :
ATGCAAAAGACTCGCATCATCTGCACCATCGGCCCCGCCACGGAATCCTACGAGATGCTGCACAAGCTCTACGAGGCCGG
GATGAGTATCGCCCGTCTCAACATGTCCCACGGCGACCACGAGTCTCACGCCAAGGTCATTCAACACATCAAATCACTCA
ACCGGAAGGTGAAGTTTCCCATTCCTATTCTCCTGGACACGCAAGGTCCGGAGATCCGTACCGGCGATCTCTCCAACGAG
CTCGACCTGCGGCAGGGTGACATTGTCTCCGTCACCACCCGCGGGCCGATGAACGTCGAGGAAAGCTCCATCCACATCAA
CTATGCCGACCTGCTGGAGGCGGTCAAGGTCGGAGACCGGATCACCGTGGACAACGGGCTGATCAACTTTGAGGTGCTGG
AGAAGCACGAACGTCACATGCGATGCCGCGTTCTGGACGGCGGGCTGCTGAAGAGCAAACGCCATGTGAATCTCCCTGGG
GTCCGTGTCAATCTCCCGTCGATCACGCAAAAAGATATCAAGGATATCCTCTTCGGTTTGGAGCGGGATGTAGACTTCAT
CGCGCTGTCGTTCGTGCGCGAGGCCGGAGATATTCAGCAACTGAAGAATTTGATGGGCGACAAGGTCGGGAAGGTAAAGA
TCGTCGCCAAGATCGAGGATCAGGAAGGAGTCCGGAACCTGGAGGCCATCATCAAGGAATCGGATGGCGTTATGGTCGCG
CGCGGGGATCTCGGGGTGGAGATCAACCTGGAGGATCTGCCGAATGTTCAGCGCACCATCGTGCGGCTGTGTGCCGAGTA
TGGCAAACGCGTCATCGTCGCGACCCATCTCCTCGAATCCATGATCCACAATCCGCATCCGACCCGCGCCGAGGTCACCG
ACGTCGCCAACGCGATCTACGAAGAAGCCGATGCCGTCATGCTGTCCGGTGAAACCACAGTGGGGAAGTATCCGGTAAAG
TGCGTCGAATTCCTCCGCAGGATTGCGCTGAAATCCGAAACGATCCCCGGATTACAGTTTGCGAAACATTTGCGCAACGC
GGAGAACAAGCAACAACTGGCGGCGGCGGTGCAGCTCGCCGAGGGGGTCAAGGCCAAAGGCATTGTCGTCATCACCAGAC
GAGGGCTCATGGCCGATCTCGTCGCAAACTGCCGCCCGTTCGCCACCAACATCTATGCCTTTACCAACATGAGCCAACCG
CGACGGACCATGATGCTCAATCGCGGCGTCCTCCCCTTCAAGATCGATTTCAGTTCAGACCCCGAAAAAACCTTACAAAC
GGCGTTCCGGATTCTGAAGGACCGCGAGGATTTTAAGGTTGGAGACAAGGTCGTCATCATCTCTGATGTCCTCGCCCAAC
AACGGGTCGACTCGATTCAGATTCGCGATGTCCCCTCTGACGACATTCTGCCCGACGTAGCCGGAGTGAGTCATTGA

Protein sequence :
MQKTRIICTIGPATESYEMLHKLYEAGMSIARLNMSHGDHESHAKVIQHIKSLNRKVKFPIPILLDTQGPEIRTGDLSNE
LDLRQGDIVSVTTRGPMNVEESSIHINYADLLEAVKVGDRITVDNGLINFEVLEKHERHMRCRVLDGGLLKSKRHVNLPG
VRVNLPSITQKDIKDILFGLERDVDFIALSFVREAGDIQQLKNLMGDKVGKVKIVAKIEDQEGVRNLEAIIKESDGVMVA
RGDLGVEINLEDLPNVQRTIVRLCAEYGKRVIVATHLLESMIHNPHPTRAEVTDVANAIYEEADAVMLSGETTVGKYPVK
CVEFLRRIALKSETIPGLQFAKHLRNAENKQQLAAAVQLAEGVKAKGIVVITRRGLMADLVANCRPFATNIYAFTNMSQP
RRTMMLNRGVLPFKIDFSSDPEKTLQTAFRILKDREDFKVGDKVVIISDVLAQQRVDSIQIRDVPSDDILPDVAGVSH

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 1e-68 41
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 1e-67 41
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 3e-68 41
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 3e-68 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pykF YP_003798437.1 pyruvate kinase VFG0479 Protein 1e-68 41