Gene Information

Name : sctN (wcw_1616)
Accession : YP_003709967.1
Strain : Waddlia chondrophila WSU 86-1044
Genome accession: NC_014225
Putative virulence/resistance : Virulence
Product : type III secretion cytoplasmic ATPase SctN
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.14
Position : 1702784 - 1704100 bp
Length : 1317 bp
Strand : +
Note : Flagellar biosynthesis/type III secretory pathway ATPase

DNA sequence :
ATGGGAATCACTGAGGATTTTGACACCATTCTTAGGGAACTTGACGAAGTCCAGCTGACGACCGTCAATGGGCGCATTAC
CGAAACTGTGGGAATGCTGATTAAAGCAGTTGTTCCCCAGGTAAAAATCGGCGAAGTGTGCCTAATCAAACGGGAAGGCG
AGCCTTTAAGAGCAGAAGTGGTGGGATTTACAAGAGACGAAGTGTTTCTCTCCCCTCTTGGAGAAATGACCGGAATTGGA
CCTTCTTCCGAGGTTATCCCAACCCGGCTGCCTCTTCATGTCAAAGTTGGCGATGCTCTCTTAGGACGCGTGCTTAATGG
CCTTGGGGAACCCTTAGACACCAACACTAAGGGACCTTTGGTTTTAAACGAAACCGCTCCTGTCATTCAAAGCCCTCCCG
ACCCGTTAAAACGGCAAAGGGTCAGCGAACCCATCTCTGTTGGGGTCCGAGTGATCGATGGCACACTGACTACAGGGCTT
GGACAGCGAGTGGGTATTTTTGCTGCCGCCGGAGGAGGTAAATCAACGCTGCTCGGCATGATTGCCCGCAACGCCGATGC
CGACATCAATGTCATCAGCCTGGTTGGTGAACGTGGACGCGAGCTGCGCGACTTCATTGAAAAAGACCTTGGACCGGAAG
GATTAAAAAAGTCCGTCTTAGTCATCTCGACTTCCGATCAATCAGCCCAGCTCAGGCTGAATGCGGCATACGTGGCAACG
GCGATTGCCGAGTATTTCCGAGACAAAGGAAAATCTGTCATTTTAATGATGGACTCTGTCACTCGATTTGCCCGCGCAAT
CCGTGAAGTGGGACTAGCAGCCGGAGAGCCTCCGGCTCGTGCAGGCTACACGCCGTCTGTATTTTCCACTCTGCCCAAGC
TTCTTGAGAGGTCAGGCCGTTCCGATGTTGGTTCGATCACAGCCTTTTATACCATTCTTGTAGCAGGAGATGATATGAAT
GAGCCTGTCGCTGATGAGGTGCGTTCCATTTTGGACGGTCACATCATCTTATCTGCAGAACTTGCGCACAAGTACCACTA
TCCGGCAGTCGATGTGCTCGCTTCAAAAAGCCGTGTCATGCAAACGATCGTATCCGAGGAGCACTTATCTCTAGCAGGTA
AAATCAACGAAGTGCTGGCCAACTACAAGAAAAATGAACTGCTCATCAAGATCGGAGAATACAAACGCGGCTCAGACAAA
GCTGGAGATTTTGCCATCGACCACATTGATAAAGTGAATAACTTTCTGAAGCAGGGAATTGAGGAAGAGTGCAGTTGGGA
CGAAACAATGCAGCTTCTTAGATCCCTATTTAAATAA

Protein sequence :
MGITEDFDTILRELDEVQLTTVNGRITETVGMLIKAVVPQVKIGEVCLIKREGEPLRAEVVGFTRDEVFLSPLGEMTGIG
PSSEVIPTRLPLHVKVGDALLGRVLNGLGEPLDTNTKGPLVLNETAPVIQSPPDPLKRQRVSEPISVGVRVIDGTLTTGL
GQRVGIFAAAGGGKSTLLGMIARNADADINVISLVGERGRELRDFIEKDLGPEGLKKSVLVISTSDQSAQLRLNAAYVAT
AIAEYFRDKGKSVILMMDSVTRFARAIREVGLAAGEPPARAGYTPSVFSTLPKLLERSGRSDVGSITAFYTILVAGDDMN
EPVADEVRSILDGHIILSAELAHKYHYPAVDVLASKSRVMQTIVSEEHLSLAGKINEVLANYKKNELLIKIGEYKRGSDK
AGDFAIDHIDKVNNFLKQGIEEECSWDETMQLLRSLFK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
lscN AAO18044.1 LscN Virulence TTSS locus Protein 1e-111 56
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-105 54
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 9e-106 54
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 8e-106 54
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 9e-105 53
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-105 53
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 3e-105 53
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 3e-105 53
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-104 53
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-104 53
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 6e-91 51
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 4e-91 51
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 1e-90 51
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 1e-90 51
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 8e-91 51
escN AAC38383.1 EscN Virulence LEE Protein 1e-91 50
escN NP_290268.1 hypothetical protein Virulence LEE Protein 1e-91 50
ECs4568 NP_312595.1 EscN Virulence LEE Protein 1e-91 50
escN AAC31513.1 L0034 Virulence LEE Protein 1e-91 50
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 1e-91 50
escN AAK26715.1 EscN Virulence LEE Protein 1e-91 50
escN AAL57542.1 EscN Virulence LEE Protein 1e-91 50
escN CAC81862.1 EscN protein Virulence LEE II Protein 1e-91 50
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 1e-87 50
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 2e-90 49
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 2e-90 49
escN YP_003236088.1 translocator EscN Virulence LEE Protein 3e-91 49
escN YP_003223475.1 translocator EscN Virulence LEE Protein 2e-91 49
escN CAI43874.1 EscN protein Virulence LEE Protein 1e-91 49
unnamed AAL06369.1 EscN Virulence LEE Protein 2e-90 49
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 2e-91 49
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 1e-94 49
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 1e-88 48
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 1e-88 48
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 1e-88 48
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 7e-89 48
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 8e-81 47
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 4e-79 47
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 2e-82 47
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 1e-82 47
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 4e-82 46
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 6e-74 45
invC AAS66861.1 InvC Not tested SSR-2 Protein 1e-72 44
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 5e-75 44
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 5e-70 44
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 5e-70 44
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 5e-70 44
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 5e-70 44
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 1e-75 44
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 1e-75 44

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
sctN YP_003709967.1 type III secretion cytoplasmic ATPase SctN VFG2144 Protein 2e-153 70
sctN YP_003709967.1 type III secretion cytoplasmic ATPase SctN VFG0048 Protein 4e-119 59
sctN YP_003709967.1 type III secretion cytoplasmic ATPase SctN VFG0390 Protein 1e-112 57
sctN YP_003709967.1 type III secretion cytoplasmic ATPase SctN VFG0192 Protein 8e-110 54
sctN YP_003709967.1 type III secretion cytoplasmic ATPase SctN VFG0515 Protein 2e-91 51
sctN YP_003709967.1 type III secretion cytoplasmic ATPase SctN VFG0812 Protein 6e-92 50
sctN YP_003709967.1 type III secretion cytoplasmic ATPase SctN VFG0730 Protein 8e-92 50
sctN YP_003709967.1 type III secretion cytoplasmic ATPase SctN VFG1254 Protein 2e-88 47
sctN YP_003709967.1 type III secretion cytoplasmic ATPase SctN VFG2331 Protein 1e-80 46
sctN YP_003709967.1 type III secretion cytoplasmic ATPase SctN VFG1778 Protein 7e-76 46
sctN YP_003709967.1 type III secretion cytoplasmic ATPase SctN VFG1008 Protein 2e-72 45
sctN YP_003709967.1 type III secretion cytoplasmic ATPase SctN VFG2499 Protein 3e-81 45
sctN YP_003709967.1 type III secretion cytoplasmic ATPase SctN VFG0555 Protein 2e-70 44
sctN YP_003709967.1 type III secretion cytoplasmic ATPase SctN VFG2459 Protein 1e-78 43