Gene Information

Name : Bsel_1350 (Bsel_1350)
Accession : YP_003699428.1
Strain : Bacillus selenitireducens MLS10
Genome accession: NC_014219
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : -
Position : 1544229 - 1545989 bp
Length : 1761 bp
Strand : +
Note : KEGG: dps:DP3116 pyruvate kinase; TIGRFAM: pyruvate kinase; PFAM: Pyruvate kinase barrel; PEP-utilising protein mobile region; Pyruvate kinase alpha/beta

DNA sequence :
ATGACTATTCGAAAGACGAAAATTGTATGTACGATTGGACCGGCGAGTGAATCTCCGGAGAAACTGACCCAGCTGATTGA
TGCGGGCATGAACGTGGCGAGACTGAACTTTTCCCATGGTGATTTTGATGAGCACGGTGCACGCATTAAATCGATCCGTG
AAGCTTCTGAAAAAGCTGGCAAGAGCGTTGCCATTTTACTCGATACAAAGGGACCTGAAATCCGTACACAAACGCTTGAA
GGCGGCATCGCAGAACTCGAGAAAGGCAAAACTGTCCGAGTTTCGATGGAAGAAGTGGTCGGGAATGCTGACCGTATTTC
TGTCACCTATCCAGGGCTTGTGAACGATGTTCACGTCGGCTCGACACTCTTGCTCGACGATGGTCTGATTGAACTGAGAG
TCACTGCCATTGAAGACAAAGAGCTCGTGACGGAAGTGGTTAACTCCGGCACATTGAAAAACAAAAAAGGTGTAAACGTA
CCGAACGTCAGCGTAAACCTGCCGGGGATTACGGAAAAAGACGCCAATGATATCGTATTCGGTATTGAACAGGATGTTGA
TTTTATCGCTGCCTCCTTCGTTCGAAGAGCGTCTGACGTTCATGAAATCCGTGAACTTCTTGAAAAGCACGGTGCTGAGC
ATATTCAGATCATTCCAAAGATCGAGAACCAGGAAGGCGTCGACAACATCGAAGAAATTCTTGAAGTGTCCGACGGCCTG
ATGGTTGCCCGTGGTGACCTCGGTGTTGAAATTCCGGCTGAAGATGTGCCGCTCGTTCAAAAAGATCTGATCAAAAAGTG
CAACCAGATCGGAAAGCCCGTCATCACGGCTACGCAAATGCTTGACAGCATGCAGCGTAACCCGCGTCCAACCCGCGCTG
AAGCGAGTGATGTTGCGAACGCGATCTTTGACGGCACAGATGCGATCATGCTGTCCGGTGAGACGGCGGCAGGGGATTAC
CCTTATGAATCCGTGACTACGATGGCAAACATTGCCCTGAAAACGGAATCAGCTCTCAAATACCGTGATATTCTCCGTAA
GAAATCAAAGGAAATCGAAAATACCGTAACGGATGCCATCAGTCAGTCTGTGTCCCACACAGCATTGAACCTGCACGCAT
CTGCCATTGTAACTGCCACTCAAAGCGGTCATACAGCACGGATGATCTCCAAGTACCGTCCGGAAGCGCCGATTGTAGCC
GTTACAAGCAGCAGTCGCGTCAACCGCGCGTTGGCACTCGTTTGGGGTGTGCATCCTCGCACGGGTCCTCAGGTGAACTC
CACGGACGAAATGCTTCGTCTGTCCGTTGAAGAAGCGCAGAAGACCGGTCTTGTGAATAATGGCGACCTCGTTGTCATCA
CTGCAGGGGTGCCGGTCGGTGAATCCGGAACGACGAACATGATGAAAGTACAGCTTGTCGGTGAAGTGGCTGCACGCGGC
CAGGGAATTGGACGCCGATCCAAAGCCGGTAAAGTCGTGGTCGCAAAGACTGGCGCAGAAGCGGTGTCAAAAGTGACGGA
AGGCGATATTCTGATCACTTCCTCAACGGATAAAGACATGATGGACGCTTTTGAGAAAGCGGGCGCTGTCATTACTGAAT
CCGGCGGTTTGACTTCACATGCAGCCGTTGTCGGTCTAAACCTCGGAATCCCGGTTATTGTCGGCGTAGATCAGGCCACA
ACGCTCTTTAACGACGGAGACGAAGTGACCGTTGATGCTCAGCAAGGAAAGATTTACAAAGGCCAGGCATCTATCTTATA
A

Protein sequence :
MTIRKTKIVCTIGPASESPEKLTQLIDAGMNVARLNFSHGDFDEHGARIKSIREASEKAGKSVAILLDTKGPEIRTQTLE
GGIAELEKGKTVRVSMEEVVGNADRISVTYPGLVNDVHVGSTLLLDDGLIELRVTAIEDKELVTEVVNSGTLKNKKGVNV
PNVSVNLPGITEKDANDIVFGIEQDVDFIAASFVRRASDVHEIRELLEKHGAEHIQIIPKIENQEGVDNIEEILEVSDGL
MVARGDLGVEIPAEDVPLVQKDLIKKCNQIGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGDY
PYESVTTMANIALKTESALKYRDILRKKSKEIENTVTDAISQSVSHTALNLHASAIVTATQSGHTARMISKYRPEAPIVA
VTSSSRVNRALALVWGVHPRTGPQVNSTDEMLRLSVEEAQKTGLVNNGDLVVITAGVPVGESGTTNMMKVQLVGEVAARG
QGIGRRSKAGKVVVAKTGAEAVSKVTEGDILITSSTDKDMMDAFEKAGAVITESGGLTSHAAVVGLNLGIPVIVGVDQAT
TLFNDGDEVTVDAQQGKIYKGQASIL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 3e-115 54
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 3e-115 54
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 3e-115 54
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 8e-115 54

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Bsel_1350 YP_003699428.1 pyruvate kinase VFG0479 Protein 1e-115 54