Gene Information

Name : Snov_3333 (Snov_3333)
Accession : YP_003695226.1
Strain : Starkeya novella DSM 506
Genome accession: NC_014217
Putative virulence/resistance : Virulence
Product : 2,3-dihydroxybenzoate-AMP ligase
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 3508074 - 3509705 bp
Length : 1632 bp
Strand : -
Note : KEGG: smt:Smal_2275 AMP-dependent synthetase and ligase; TIGRFAM: 2,3-dihydroxybenzoate-AMP ligase; PFAM: AMP-dependent synthetase and ligase

DNA sequence : 
GTGAAGCCTCTGTCGCAGGTCTGGCCGGACGACTTCGCCGCCCGCTACCGCGCCAAGGGCTATTGGCGCGGCGAGACCTT
CTCCTCCTTCCTGCGCGCCCGCGCGGCCGAGCTGCCGGACCATCTCGCCGTGGTCGGCGGTGAACACCGCTGGACCTTCG
CCGAGCTCGAAGCCCGCGCGGAAAACATCGCCCGCGGCCTGCTGGTGCTGGGGTTGAACCCCGGCGACCGGGTGGTGGTG
CAGCTTCCGAACATCGCCGAATTCCTCTCCGTGGTGTTCGGCCTGTTCCGCGCCCGGCTCATCCCGGTCTATGCGCTCCC
CGCCCACCGCATCGTCGAAATCACCCATTTCGCCCACACCGCTGAGGCGCGCGGCTACCTCATTGCCGCCAGCCATGACG
GCTTCGACTACCGCACGCTGGCGCAGGAGGTGCGCGAGCAGGTACCCGCGGTGGAGCATGTCGTGGTGGTCGGCGAAGGC
GGGCCGTTCACCGCGCTCGACGCGCTGCCGGCGGGCAGGGAGATCGCGCTACCAGAGGATGCCGATCCGTCCGAGGTCGC
TTTCCTGCAAATCTCGGGCGGCTCGACGGGGCTCTCCAAGCTCATCCCGCGCACGCATGACGACTATATCTACTCGTTCC
GCGCCAGCGCCGAGATATGCAAGCTAACGCCCGAGAGCGTCTATCTCGCCGCGCTTCCCGTCGCTCATAATTTCCCGATG
TCATCGCCCGGCGTGTTCGGCGCGCTCTATGCCGGCTCGCGCGTGGTGCTCTCGCCCTCGCCGAGCCCGGAGGTCGCCTT
CCCGCTGATCGCGCGCGAGGGCGTGACCATCACCGGCCTTGTGCCGCCGCTCGCGCTGCTCTGGCTGCAGGCGGCGCCGA
GCACCGCGCACGACCTCTCCTCGCTGCAGGTGCTGCAGGTCGGCGGCGCCAAGTTCCTCACCGAATCCGCCCGCCGGGTG
AAGCCGGTGCTCGGCTGCACGCTCCAGCAGGTGTTCGGCATGGCCGAGGGGCTGGTGAACTACACCCGCCTGGACGACGA
CGAGGAGAGCATCGTCTCCACGCAGGGCCGGCCGATCAGCCCCGACGACGAGGTGCTGATCCTCGACGACGAGGGCAACC
CCGTGCCCGAGGGCGAGGCCGGCAACCTGCTGACGCGCGGCCCCTACACGATCCGCGCCTATCACAATGATGACGGGGCG
AATGCCCGCTCCTTCACGCCGGACGGCTTCTACCGCACCGGCGACGTGGTGAAGCGCACGCCCGACGGTTATCTCGTCGT
TCAGGGGCGGGCGACCGACCACATCAACCGCGCCGGCGAGAAGATCTCGGCCGAGGAGATCGAGGATCATCTCCTCGCCC
ATCCCGGCGTGTTCGATGCGGCAGTGGTCTCGCTGCCGGACGAATTCCTCGGCGAGCGCTCTTGCGCCTTCGTGATCCCG
CAGGGCGAGAAGCCCAAGGCGGCAGCGTTGAAGGCGTGGATGCGCACGCGCGGCCTTGCCGACTTCAAGGTGCCGGACCA
GGTCGTCTTCGTCGACGCCTTCGAGACCACGGCGGTGGGCAAGGTGAGCCGCAAGGAGTTGCGCGCCAGCCTGCGCCGGC
GCTTCCTCGAAGCCGCCGAGGCGGAGAAGTGA

Protein sequence : 
MKPLSQVWPDDFAARYRAKGYWRGETFSSFLRARAAELPDHLAVVGGEHRWTFAELEARAENIARGLLVLGLNPGDRVVV
QLPNIAEFLSVVFGLFRARLIPVYALPAHRIVEITHFAHTAEARGYLIAASHDGFDYRTLAQEVREQVPAVEHVVVVGEG
GPFTALDALPAGREIALPEDADPSEVAFLQISGGSTGLSKLIPRTHDDYIYSFRASAEICKLTPESVYLAALPVAHNFPM
SSPGVFGALYAGSRVVLSPSPSPEVAFPLIAREGVTITGLVPPLALLWLQAAPSTAHDLSSLQVLQVGGAKFLTESARRV
KPVLGCTLQQVFGMAEGLVNYTRLDDDEESIVSTQGRPISPDDEVLILDDEGNPVPEGEAGNLLTRGPYTIRAYHNDDGA
NARSFTPDGFYRTGDVVKRTPDGYLVVQGRATDHINRAGEKISAEEIEDHLLAHPGVFDAAVVSLPDEFLGERSCAFVIP
QGEKPKAAALKAWMRTRGLADFKVPDQVVFVDAFETTAVGKVSRKELRASLRRRFLEAAEAEK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE CAA21394.1 - Virulence HPI Protein 4e-93 46
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 4e-94 46
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 3e-93 46
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 3e-94 46
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 5e-93 46
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 4e-93 46
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 4e-93 46
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 4e-93 46
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 4e-93 46

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Snov_3333 YP_003695226.1 2,3-dihydroxybenzoate-AMP ligase VFG0168 Protein 6e-115 50
Snov_3333 YP_003695226.1 2,3-dihydroxybenzoate-AMP ligase VFG1817 Protein 8e-106 48
Snov_3333 YP_003695226.1 2,3-dihydroxybenzoate-AMP ligase VFG0932 Protein 1e-99 45