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Name : nifJ1 (PFREUD_01840) Accession : YP_003687150.1 Strain : Propionibacterium freudenreichii CIRM-BIA1 Genome accession: NC_014215 Putative virulence/resistance : Virulence Product : pyruvate synthase/pyruvate-flavodoxin oxidoreductase Function : - COG functional category : C : Energy production and conversion COG ID : COG0674 EC number : 1.2.7.1 Position : 222815 - 226582 bp Length : 3768 bp Strand : + Note : Oxidoreductase required for the transfer of electrons from pyruvate to flavodoxin, which reduces nitrogenase , pyruvate + CoA + oxidized flavodoxin = acetyl-CoA + CO2 + reduced flavodoxin DNA sequence : ATGACTACAACTACCCGTGGGCCGGTTCCCGGCTCGAATGGCATGCCCGCCAATCCAGGTCTGAGCGGCGAGGCCGCCAC CGCAACCCCGTCACCCGTTGACGTCGCTGCCGGCGCCAAGGACGCTGCCGATGAGCTGGCCCAGTCACGACGCGAGCAGG ACATCACCCATCAGATGATCTGCGACGGCAACACCGCCGCCTCTGATGTGGCCTTCCGCATCAATGAGCTGTGCTCGATC TACCCGATCACGCCGAGCTCCCCGATGGCCGAACTGGCCGACGAGTGGAGTGCCCGCGACCGCATGAACATCTGGGGCCA GGTGCCCCATGTGATGGAGATGCAGTCGGAGGCCGGCGCGGCCGGTGCCATGCACGGCTCCCTGCAGGGCGGCGCCCTGG CGACCACCTTCACGGCGTCGCAGGGCCTGCTGCTGATGATCCCGAACATGTACAAGATCTCCGGTGAGCTCACCTCCACG GTGATGCACGTCGCCGCGCGCTCGCTGGCCACCCAGGGCCTGTCGATCTTCGGTGACCACCAGGACGTGATGGCCTGTCG CCAGACCGGTTGGGCGATGCTGTGCTCCACCGGCGTGCAGCAGTGCCATGACAACGCCCTGATCTCCCAGGTCGCCACGC TGCGTTCGCGCGTGCCGTTCATGCACTTCTTCGACGGCTTCCGCACCAGCCATGAGCTCAACACCTGCATCCAGCTCACC GACGACCAGCTGCGTTCGATGGTGCCCGATGCGCTCGTGCGCGAGCACCGCGAGCGGGCCCTGTCGCCCGACAACCCGTT CATCCGTGGCACCGCCCAGAACGCCGACGTGTACTTCCAGGGCCGCGAGGCCGGCAACAAGTACTACGACTCGGTTCCGG GCATCGTGCAGGACGCGATGGACGAGTTCGCCGCCATGACCGGCCGTCAGTACCACCTGGCCGACTACTACGGCGCGCCC GACGCCGATCGCGTCATCGTGATCATGGGCTCGGGTGCCGAGACCGTGCAGCAGACCGTCAGCAAGCTCAATGAGCAGGG CGAGAAGGTCGGCCTGGTGGTCATCCGCCTGTACCGTCCGTTCCCGACGCAGGCCGTGCTGGACTGCATTCCCGCATCGG TCAAGAAGATCGCCGTGCTCGACCGCACCAAGGAGCCGGGCTCCAACGGTGAGCCCCTGTTCCTCGACGTGGTCTCGGCA GTCTCCGAGGCCTATTCGAACGGCGAGCGCGACAACCTGCCCGCCATCATCGGTGGCCGCTACGGCCTGTCGAGCAAGGA GTTCACGCCGGGCATGTGCGCCGCCGTGTACGACGAGCTCGCCAAGGACAAGCCGAAGCGTCGCTTCACCGTCGGCATCA CCGACGATGTGACGCACCTGTCGATCCCGTGGGACGCCTCGCTCGACCTGGAGGACCCCGAGACCTCGCGTGCAGTGTTC TACGGCATCGGTGCTGACGGCACCGTCGGCGCCAACAAGAACACCATCAAGATCCTCGGCTCCGAGCCGGGCACCTACGC GCAGGGCTACTTCGTCTACGACTCGAAGAAGTCCGGCGGCCGCACCACCTCGCACCTTCGCTTCGGACCCGATCCGATCA AGGCCCCCTACCTGGTCAACCAGGCCGGCTTCATCGGCGTGCACCACTGGGCCGACCTTGAGCGCATCGACGTGCTGGCG TTCGCCCGCAAGGGCACCACGGTGCTGATCAACAGCCCGTACCCCGCCGAGGACGTCTGGGGCCATCTGCCGGCCCCGAT GCAGAAGAAGATCATCGACCTCGACCTGCAGGTGTATGCGATCGACGCCGGTGAGGTGGCCCGTTCGGTGGGCCTGGGCA ACCGCACCAACACGGTGCTGCAGACCTGCTACTTCAAGATCAGTGGCGTGCTTCCCGAGGACCACGCGATCGAGGCCATC AAGAACTCGATCACCAAGACCTACGCGAAGAAGTCGATGGAGATCGTGGAGAAGAACCACGCCGCCGTCGACGCCGCCCT GGAGCACCTGCACAAGATCGACGTGCCGGCCAAGGTCACCTCCACCGAGGACTACCTGCCGCCCGTGCCGTCGTTCGCGC CTGACTTCGTCAAGGACGTCACCGCGGCCATGATGACCGAGCAGGGCGAGTCGCTGCCGGTGAGCAAGCTGCCGGCCGAT GGTTCGTTCCCCTCGGGCACCACGCAGTACGAGAAGCGCAATGTGTCCGAGATCATCGCGGTCTGGGACCAGGACAACTG CATCCAGTGCGGCAACTGCGCCTTCGTCTGCCCGCACGGCGTGCTGAGGGCCAAGTACTACAAGCCCGATGTGCTCGACG ATGCGCCGAAGTCGTTCCAGGCGGTTCCGCTGAATGCGGCCGGCCTGCCCGACGAGATGTACACCCTGCAGGTGTTCGCC GAGGACTGCACCGGTTGTGGCCTGTGCGTCGAGGCCTGCCCCGTGCATCCCATCGGTGGCGACCCCGAACGCAAGGCGAT CAACCTGGATTCCGTGCTCGACCGCACCAACGAGCGGGCGAACGTGGAGTTCTTCCAGAAGATCCCCGAGCCCCCGCGCA CCCGCGTGAACTACGGTGCCGTGCGTGGCGCCCAGTTCCTGCAGCCGCTGTTCGAGTTCAGCGGTGCCTGCCCGGGTTGT GGCGAGACGCCGTACCTCAAGCTGCTCACCCAGCTGTTCGGCGACCGCGCCACCGTGGCGAATGCCACCGGCTGCTCGTC CATCTACGGCGGCAACCTGCCGACCACCCCGTGGGCGAAGAACAAGGAGGGACGCGGCCCGGCCTGGAGCAACTCATTGT TCGAGGACAACGCCGAGTTCGGCCTTGGCATGCGCCTGGCGGCCGACCTGCACAACGAACTGGCCCGTCGGCGCGTTGAC GAGCTGTCCGATGCGATCAACGACCCCGAGCTGGTCGATCAGCTGCTGAACGCCCCGCAGGCGCAGGAGTCCGATCTGCA CGCCCAGGCCGAGCGCGTCGACGCCCTGCAGGATCGCCTGACCGACCTGGTCAACGATCCGAACGTGGACGCCGACACCA AGGCCAAGGTCGAGGACCTGCGGTCGGTGGCCGACAACCTGCTGCGTCGTTCCGTGTGGATCGTCGGTGGCGACGGTTGG GCCTACGACATCGGTTCGGGCGGCCTTGACCATGTGCTGTCCACCGGACGCAATGTCAATGTGCTGGTGCTCGACACCGA GGTCTACTCCAATACCGGTGGCCAGGCCTCGAAGTCGTCGCCCATGGGCGCGATCGCGAAGTTCGCGACCGCCGGCAAGC GCACGAACAAGAAGGACATCGCCATGCAGGCCGTGTCCTACGGCGACGTCTATGTCGCCCGCGTGGCGTTCGGTGCCGAC CCGGAGCAGACGCTGAAGGCGTTCCGTGAGGCCGAGGCCTACCCCGGCCCCAGCCTGATCATCGCCTACAGCCACTGCAT CAGCCATGGCTACAACCTGCGCAAGGGCCTGGACCAGCAGTACAAGGCAGTGGCCTCCGGTCACTGGCCGCTGATCCGGT ACAACCCGGAGGTTCGCGACTCGGGTGGTAACCCGTTCCTGCTCGACTCGGCCCGTCCGCGCATCTCGCTGATGGACTAC CGCAAGACCGAGCTGCGCTTCAAGATGCTGATGGTCAAGGATCCGGAAGAGGCCAAGCGCCTCAATGACCTCAGCCAGGA GCAGGTGACCAGGCGTTTCGCCGACTACGAGGAAATGGCCTCACGTCCGGCCGAGATGTTCGCCACCGACGCACGGAGGG ATGTCTGA Protein sequence : MTTTTRGPVPGSNGMPANPGLSGEAATATPSPVDVAAGAKDAADELAQSRREQDITHQMICDGNTAASDVAFRINELCSI YPITPSSPMAELADEWSARDRMNIWGQVPHVMEMQSEAGAAGAMHGSLQGGALATTFTASQGLLLMIPNMYKISGELTST VMHVAARSLATQGLSIFGDHQDVMACRQTGWAMLCSTGVQQCHDNALISQVATLRSRVPFMHFFDGFRTSHELNTCIQLT DDQLRSMVPDALVREHRERALSPDNPFIRGTAQNADVYFQGREAGNKYYDSVPGIVQDAMDEFAAMTGRQYHLADYYGAP DADRVIVIMGSGAETVQQTVSKLNEQGEKVGLVVIRLYRPFPTQAVLDCIPASVKKIAVLDRTKEPGSNGEPLFLDVVSA VSEAYSNGERDNLPAIIGGRYGLSSKEFTPGMCAAVYDELAKDKPKRRFTVGITDDVTHLSIPWDASLDLEDPETSRAVF YGIGADGTVGANKNTIKILGSEPGTYAQGYFVYDSKKSGGRTTSHLRFGPDPIKAPYLVNQAGFIGVHHWADLERIDVLA FARKGTTVLINSPYPAEDVWGHLPAPMQKKIIDLDLQVYAIDAGEVARSVGLGNRTNTVLQTCYFKISGVLPEDHAIEAI KNSITKTYAKKSMEIVEKNHAAVDAALEHLHKIDVPAKVTSTEDYLPPVPSFAPDFVKDVTAAMMTEQGESLPVSKLPAD GSFPSGTTQYEKRNVSEIIAVWDQDNCIQCGNCAFVCPHGVLRAKYYKPDVLDDAPKSFQAVPLNAAGLPDEMYTLQVFA EDCTGCGLCVEACPVHPIGGDPERKAINLDSVLDRTNERANVEFFQKIPEPPRTRVNYGAVRGAQFLQPLFEFSGACPGC GETPYLKLLTQLFGDRATVANATGCSSIYGGNLPTTPWAKNKEGRGPAWSNSLFEDNAEFGLGMRLAADLHNELARRRVD ELSDAINDPELVDQLLNAPQAQESDLHAQAERVDALQDRLTDLVNDPNVDADTKAKVEDLRSVADNLLRRSVWIVGGDGW AYDIGSGGLDHVLSTGRNVNVLVLDTEVYSNTGGQASKSSPMGAIAKFATAGKRTNKKDIAMQAVSYGDVYVARVAFGAD PEQTLKAFREAEAYPGPSLIIAYSHCISHGYNLRKGLDQQYKAVASGHWPLIRYNPEVRDSGGNPFLLDSARPRISLMDY RKTELRFKMLMVKDPEEAKRLNDLSQEQVTRRFADYEEMASRPAEMFATDARRDV |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| unnamed | AFJ97267.1 | pyruvate ferredoxin | Virulence | Not named | Protein | 0.0 | 47 |
