Gene Information

Name : M301_1005 (M301_1005)
Accession : YP_003673966.1
Strain : Methylotenera versatilis 301
Genome accession: NC_014207
Putative virulence/resistance : Virulence
Product : flagellar protein export ATPase FliI
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.14
Position : 1128842 - 1130374 bp
Length : 1533 bp
Strand : +
Note : SMART: AAA ATPase; TIGRFAM: flagellar protein export ATPase FliI; ATPase, FliI/YscN family; KEGG: mmb:Mmol_0930 ATPase, FliI/YscN family; PFAM: H+transporting two-sector ATPase alpha/beta subunit central region; H+transporting two-sector ATPase alpha/beta

DNA sequence :
ATGAATTCTCCAGAGCTCAGTTCGCTGGCATTAGATAAACAGGTTGCTCAGCCACACCCTAGCATGCCTGATTTAGAGCA
ACTGCGCGAAGCTACTCCAGACTCAACTCAGCCAAAAGTTGATATGCAAAATATCCATCGCCAACGCTGGCATGACCATA
TTCGTGATTGCAAAGAGATTCTACGCATTGTAGAGCCCTTAGAAATAGCAGGGCGCATCACTAAGTTAACAGGCTTAGTC
ATGGAGGCCGCTGGTATTAAGCTACCGATTGGCAGTGCATGTTATGTGCCGCTTGCTGAAGGTCGTCGCGTAGAAGCTGA
AGTTGTAGGTTTTGATGGTGAGCGCATGTTATTAATGCCACAAAGCAGTGTCGATGGTGTGATGCCAGGTGCCAAAGTAT
TTGCTTTAGAAGTTGCAGAAGCTTTGCCTAAGCCTCACCACGGTAATCCGCCACGCAGGAGAGTAACAGATCGCGCACGT
CATCTACCTGTTGGCAATGAGTTGCTAGGTCGTGTAGTGGATGGTGCAGGCAATCCTTTAGACGATCTCGGAAAAATTGT
AGCTGCCCAAAGCTCACCACTGAATGCACGACCAATGAACCCGTTGATGCGAGCTCCTATTGAAGAGATTTTAGATGTAG
GTGTACGTGCCATTAACAGTATGCTCACAGTTGGCCGTGGTCAACGCATGGGCTTATTTGCTGGTTCTGGTGTTGGTAAA
AGCGTGCTGCTAGGCATGATGGCGCGCTACACCACCGCTGATGTGATTGTGGTTGGTTTAATCGGTGAACGGGGACGTGA
GGTTCAGGAGTTTATTGAACAAATCTTAGGCCCTGTTGGTTTGGCACGCTCTGTTGTAGTAGCGGCACCCGCAGATGCGC
CAGCTTTAATGCGCCTACAAGGTGCAAATTACGCGACAACTATCGCCGAGAATTTTCGTGATCAAGGCAAAAACGTATTG
TTGATTATGGATTCATTAACACGTTATGCCATGGCGCAGCGTGAAATTGCATTAGCCATTGGCGAGCCGCCAGCGACTAA
AGGTTATCCACCATCAGTATTCGCTAAATTACCAGCATTGGTTGAGCGTACTGGAAATGGTAAACGTGGTGAGGGTTCAA
TTACAGCTTTTTATACGGTGCTGACAGAAGGCGACGATCAACAAGATCCAATTGCCGATGCGGCGCGTGCTATTTTGGAT
GGTCATATCGTATTAAGTCGTAGCTTGGCTGAGAGCGGACATTACCCAGCCATCGATGTAGAACAATCTATCAGCCGTGC
GATGCACAATATTACCAGTCCAGAACATCAAAAGCTTGCACGCAAATTAAAGCAGCTCAACTCACGATATATGCGCAGTG
TGGATTTAATCAATGTCGGCGCCTATGAAGCGGGTAGTGATGCCGTGCTGGATCAAGCGATTGCTAGACACGACAAGATT
GATGCATTTTTGCAGCAAGGTATTTCTGAGCGATCAGGGGTTGAAGAGAGTATTGGCCATCTAGCGCATGTTTTAGATGG
CTTAGTTAATTAA

Protein sequence :
MNSPELSSLALDKQVAQPHPSMPDLEQLREATPDSTQPKVDMQNIHRQRWHDHIRDCKEILRIVEPLEIAGRITKLTGLV
MEAAGIKLPIGSACYVPLAEGRRVEAEVVGFDGERMLLMPQSSVDGVMPGAKVFALEVAEALPKPHHGNPPRRRVTDRAR
HLPVGNELLGRVVDGAGNPLDDLGKIVAAQSSPLNARPMNPLMRAPIEEILDVGVRAINSMLTVGRGQRMGLFAGSGVGK
SVLLGMMARYTTADVIVVGLIGERGREVQEFIEQILGPVGLARSVVVAAPADAPALMRLQGANYATTIAENFRDQGKNVL
LIMDSLTRYAMAQREIALAIGEPPATKGYPPSVFAKLPALVERTGNGKRGEGSITAFYTVLTEGDDQQDPIADAARAILD
GHIVLSRSLAESGHYPAIDVEQSISRAMHNITSPEHQKLARKLKQLNSRYMRSVDLINVGAYEAGSDAVLDQAIARHDKI
DAFLQQGISERSGVEESIGHLAHVLDGLVN

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 1e-79 49
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-79 47
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-79 47
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 6e-80 47
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 8e-80 47
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 1e-81 47
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-81 47
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 1e-81 47
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-81 47
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 5e-82 47
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 4e-70 46
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 6e-70 46
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 8e-70 46
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 4e-70 46
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 4e-70 46
lscN AAO18044.1 LscN Virulence TTSS locus Protein 2e-78 45
unnamed AAL06369.1 EscN Virulence LEE Protein 2e-73 45
escN AAC38383.1 EscN Virulence LEE Protein 5e-73 45
escN CAI43874.1 EscN protein Virulence LEE Protein 2e-73 45
escN CAC81862.1 EscN protein Virulence LEE II Protein 3e-73 45
escN AAK26715.1 EscN Virulence LEE Protein 3e-73 45
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 4e-73 45
escN AAL57542.1 EscN Virulence LEE Protein 3e-73 45
escN YP_003236088.1 translocator EscN Virulence LEE Protein 3e-73 45
escN YP_003223475.1 translocator EscN Virulence LEE Protein 2e-73 45
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 1e-66 44
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 1e-70 44
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 1e-70 44
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 2e-68 44
escN AAC31513.1 L0034 Virulence LEE Protein 8e-73 44
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 8e-73 44
escN NP_290268.1 hypothetical protein Virulence LEE Protein 1e-72 44
ECs4568 NP_312595.1 EscN Virulence LEE Protein 1e-72 44
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 4e-75 43
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 6e-65 42
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 2e-71 42
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 6e-71 42
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 2e-71 42
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 3e-63 42
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 3e-63 42
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 5e-68 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
M301_1005 YP_003673966.1 flagellar protein export ATPase FliI VFG2499 Protein 2e-133 62
M301_1005 YP_003673966.1 flagellar protein export ATPase FliI VFG2331 Protein 1e-125 61
M301_1005 YP_003673966.1 flagellar protein export ATPase FliI VFG1254 Protein 5e-115 60
M301_1005 YP_003673966.1 flagellar protein export ATPase FliI VFG0515 Protein 3e-70 46
M301_1005 YP_003673966.1 flagellar protein export ATPase FliI VFG0192 Protein 2e-76 46
M301_1005 YP_003673966.1 flagellar protein export ATPase FliI VFG0048 Protein 1e-80 45
M301_1005 YP_003673966.1 flagellar protein export ATPase FliI VFG2144 Protein 5e-79 45
M301_1005 YP_003673966.1 flagellar protein export ATPase FliI VFG0390 Protein 2e-76 45
M301_1005 YP_003673966.1 flagellar protein export ATPase FliI VFG0730 Protein 3e-73 45
M301_1005 YP_003673966.1 flagellar protein export ATPase FliI VFG0812 Protein 5e-73 44