Gene Information

Name : GC56T3_3124 (GC56T3_3124)
Accession : YP_003672627.1
Strain : Geobacillus sp. C56-T3
Genome accession: NC_014206
Putative virulence/resistance : Resistance
Product : mercuric reductase
Function : -
COG functional category : C : Energy production and conversion
COG ID : COG1249
EC number : -
Position : 3280598 - 3282238 bp
Length : 1641 bp
Strand : -
Note : KEGG: gyc:GYMC61_3185 mercuric reductase; TIGRFAM: mercuric reductase; PFAM: FAD-dependent pyridine nucleotide-disulphide oxidoreductase; Heavy metal transport/detoxification protein; pyridine nucleotide-disulphide oxidoreductase dimerisation region

DNA sequence :
ATGAAAACATATCGAATGACCGTCCAAGGCATGACATGCACCGGCTGTGAACAACATGTCGCCAGAGCGCTGGGGGCGAT
TGGGGCCAAGCTGATGGATGTCAGCTTCCAGCGCGGTGAAGCGGTATTCGCGCTGCCTGAGGGCACGGACGTTGACATGG
CGAGACAAGCGGTCAAAAACGCCCATTACGAGCCGGGGGAAGCCGAGGAGATCACCGCATCAGCCAGATCTGTCATCGAT
GGCGAGGGAGAGTATGACTACATCGTCATCGGTTCCGGCGGGGCGGCGTTTTCCTCGGCGATCGAAGCGGTGAAATACGG
GGCGAAAGTGGCGATGGTCGAACGTGGCACGGTCGGGGGGACGTGCGTCAATATCGGCTGCGTGCCGTCAAAGACATTGC
TTCGGGCTGGTGAAATCAATGCGTTGGCGCGAAATCATCCGTTTCTCGGCCTGCATACATCCGCTGGTCCGGTTGATTTG
GCGCCGTTAGTGAAGCAAAAAAATGAGCTGGTCGAGCAGCTTCGCCAAGCGAAATATGCAGACTTGATCGGAGAGTATGG
GTTCGATTTCATCCAAGGGGAAGCCCGATTTGTTGGGCGCCAAACGATTGAAGTGAACGGCCAAACGCTGTCGGCGAAAC
GGTTTTTGATCGCGACAGGCGCTTCCCCGGCTGTGCCGGATATTCCGGGGCTTCATGATGTCGACTACTTGACCAGCACG
ACGTTGCTCGAGCTGAAAAAGGTGCCGAAGCGGCTCGCCGTCATCGGCGCCGGCTACATCGGTATGGAGTTGGGACAACT
GTTCCACCATCTCGGCTCGGAGGTGACGTTGATGCAGCGAAGCCCACGCCTGCTGAAAGAGTATGACCCGGAAATTTCCG
AAGCGGTGGCGCGGGCGCTCACGGAACAAGGCATCCGCATCATCACCGGCGCTTCGTTTGAACGGGTCGAACAAGACGGG
AATACGAAAAAAGTGTATGTGAATGTAGACGGCCGCACCCGGGTCATCGAAGCGGACGAATTGCTCGTGGCTGCCGGGAG
AACGCCGAATACGGCGGCGTTGAACTTGCCAGCAGCCGGTGTGGAAGTCGGGGCGCGCGGGGAAATTTTGATCGACGAAT
ACACACGGACGACGAACCCGTCGATTTATGCGGCAGGCGATGTCACGCTCGGCCCGCAGTTCGTCTATGTCGCGGCCTAT
CAGGGAGCCGTTGCCGCGGCCAATGCCGTCGGCGGGCTGAACAAGCGCTGGGATACGGCCGTCGTTCCGGCGGTGACGTT
CACCCATCCGGCCATCGCCACCGTCGGGTTGACCGAACAGCGGGCGAAAGAAAACGGGTATGACGTGAAAACGTCCGTCC
TGCCGCTTGAGGCGGTGCCGCGCGCGATCGTCAACCGGGAAACGACGGGGGTGTTTAAACTTGTCGCTGAGGCCCGTACG
GGCAAGCTGCTAGGCGCCCACGTTGTTGCGGACAATGCCGGCGAGGTCATTTACGCTGCGACGTTGGCGATTCAATTCGG
ATTAACGATCGACGACCTTCGCCGCACGTTGGCTCCGTATTTAACGATGGCGGAGGGGCTGAAGCTGGCGGCGCTTACGT
TTGACCAAGATGTTTCGAAGTTATCGTGCTGCGCGGGATGA

Protein sequence :
MKTYRMTVQGMTCTGCEQHVARALGAIGAKLMDVSFQRGEAVFALPEGTDVDMARQAVKNAHYEPGEAEEITASARSVID
GEGEYDYIVIGSGGAAFSSAIEAVKYGAKVAMVERGTVGGTCVNIGCVPSKTLLRAGEINALARNHPFLGLHTSAGPVDL
APLVKQKNELVEQLRQAKYADLIGEYGFDFIQGEARFVGRQTIEVNGQTLSAKRFLIATGASPAVPDIPGLHDVDYLTST
TLLELKKVPKRLAVIGAGYIGMELGQLFHHLGSEVTLMQRSPRLLKEYDPEISEAVARALTEQGIRIITGASFERVEQDG
NTKKVYVNVDGRTRVIEADELLVAAGRTPNTAALNLPAAGVEVGARGEILIDEYTRTTNPSIYAAGDVTLGPQFVYVAAY
QGAVAAANAVGGLNKRWDTAVVPAVTFTHPAIATVGLTEQRAKENGYDVKTSVLPLEAVPRAIVNRETTGVFKLVAEART
GKLLGAHVVADNAGEVIYAATLAIQFGLTIDDLRRTLAPYLTMAEGLKLAALTFDQDVSKLSCCAG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
SE0085 NP_763640.1 dihydrolipoamide dehydrogenase Not tested SCCpbp4 Protein 2e-178 65
merA BAB47642.1 mercuric reductase Not tested Type-III SCCmec Protein 2e-178 65
merA AAN62178.1 mercuric (Hg(II)) reductase Not tested PAGI-2(C) Protein 2e-83 45
merA ABQ57371.1 MerA Not tested SGI1 Protein 8e-84 44
merA AGK07021.1 MerA Not tested SGI1 Protein 8e-84 44
merA ACN81005.1 MerA Hg(II) mercuric ion reductase Not tested AbaR5 Protein 1e-82 44
merA AGK07079.1 MerA Not tested SGI1 Protein 8e-84 44
unnamed ABR13401.1 mercuric reductase Not tested PAGI-5 Protein 5e-80 43
merA AET25397.1 MerA Not tested PAGI-2(C) Protein 1e-80 43
merA AFG30120.1 MerA Not tested PAGI-2 Protein 1e-80 43
merA YP_006098387.1 mercuric ion reductase Not tested Tn2411 Protein 2e-80 43
merA CAJ77060.1 Mercuric ion reductase Not tested AbaR1 Protein 6e-83 43

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
GC56T3_3124 YP_003672627.1 mercuric reductase BAC0224 Protein 0.0 71
GC56T3_3124 YP_003672627.1 mercuric reductase BAC0651 Protein 1e-178 65
GC56T3_3124 YP_003672627.1 mercuric reductase BAC0652 Protein 3e-81 43
GC56T3_3124 YP_003672627.1 mercuric reductase BAC0650 Protein 8e-77 42
GC56T3_3124 YP_003672627.1 mercuric reductase BAC0653 Protein 2e-80 41