Gene Information

Name : Tpau_0524 (Tpau_0524)
Accession : YP_003645504.1
Strain : Tsukamurella paurometabola DSM 20162
Genome accession: NC_014158
Putative virulence/resistance : Resistance
Product : D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein
Function : -
COG functional category : C : Energy production and conversion
COG ID : COG1052
EC number : -
Position : 553301 - 554299 bp
Length : 999 bp
Strand : -
Note : PFAM: D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding; D-isomer specific 2-hydroxyacid dehydrogenase catalytic region; KEGG: fre:Franean1_7138 D-isomer specific 2- hydroxyacid dehydrogenase NAD-binding

DNA sequence :
ATGACCCAGACAACGATCGCCCCGCTCGCGGCTCGTGCGCCGCGCCCGACCGGCATCACCGTCTACGGCTGCGCGGACGA
CGAAGCCGCGTTGTTCCGGAAGGCGGCGCCCCGGTGGGGAATCCCTCTCATCATCACCGAGCTACCCCTGTCCGAGGAGA
ACGTCATGCTGGCGTTCGGCCGCAGGACCATCAGCGTCGGCCACCGCACGCCCGTAACCCATCCCGTTCTGCTGGCACTG
AGCCGGATCGGGGTTCGCTACGTATCCACCCGAAGCGCAGGGTTCGACCACATCGACGTCGAGTACGCGGCCCAGTTGGG
GATCACCGTGGGCAACGTCGACTACTCGCCGGACAGCGTGGCCGATTACACCCTGATGCTGATGCTCATGGCGATCCGCG
ACGCCAAAGCGGTGATGCGGCGCACCGACTCCCACGACTACCGGCTCAGCCGACACCGCGGCCGTGAGCTGCGCGACCTC
ACCGTCGGCGTGATCGGCGCCGGTCGCATCGGATCGGCGGTCGTCGAGCGTCTCACCGGATTCGGCTGCCGTGTCCTGAC
CGCGGACACCCGCCCTGGCGATGACCATGTGAGCCTCGACCACCTCCTGGCGAGCAGCGACATCATCACACTGCACACCC
CACTCACCGCGGACACTCATCACCTGCTCGATGCGGCGCGGATCCACCGAATGAGGCCGGGAGCCATCGTGGTCAACACC
GGCCGCGGCGCCCTCATCGACACCGATGCTCTCGTCGACGCCCTGGAGGCCGGACATCTCGGCGGCGCAGCCCTCGACGT
GCTGGAGGGCGAGCACGGCATCTTCTACACCGATCGTCGCGACCGGCCCGTGCAGAACTCGGCGCTGCTGCGGTTGCAGC
ACTTGCCGAACGCGATCATCAGCCCGCACGCCGCCTACTTCACCGATCACGCGCTCCGCGACACCGTGCACAACAGCCTG
CTGAACTGCCTGACTTTCGAGGAGAACGAGAACTCATGA

Protein sequence :
MTQTTIAPLAARAPRPTGITVYGCADDEAALFRKAAPRWGIPLIITELPLSEENVMLAFGRRTISVGHRTPVTHPVLLAL
SRIGVRYVSTRSAGFDHIDVEYAAQLGITVGNVDYSPDSVADYTLMLMLMAIRDAKAVMRRTDSHDYRLSRHRGRELRDL
TVGVIGAGRIGSAVVERLTGFGCRVLTADTRPGDDHVSLDHLLASSDIITLHTPLTADTHHLLDAARIHRMRPGAIVVNT
GRGALIDTDALVDALEAGHLGGAALDVLEGEHGIFYTDRRDRPVQNSALLRLQHLPNAIISPHAAYFTDHALRDTVHNSL
LNCLTFEENENS

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
vanHB YP_008394258.1 VanH protein, D-specific alpha-keto acid dehydrogenase Not tested Not named Protein 1e-75 54
EF2295 NP_815952.1 D-specific alpha-keto acid dehydrogenase Not tested Not named Protein 5e-76 54

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Tpau_0524 YP_003645504.1 D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein AF155139.2.orf4.gene Protein 1e-81 54
Tpau_0524 YP_003645504.1 D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein NC_014475.1.orf2.gen Protein 1e-80 54
Tpau_0524 YP_003645504.1 D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein NC_005054.2598279.p0 Protein 1e-80 54
Tpau_0524 YP_003645504.1 D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein AF310956.2.orf4.gene Protein 3e-76 54
Tpau_0524 YP_003645504.1 D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein U35369.1.gene5.p01 Protein 2e-76 54
Tpau_0524 YP_003645504.1 D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein FJ349556.1.orf3.gene Protein 3e-78 52
Tpau_0524 YP_003645504.1 D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein AF130997.1.orf3.gene Protein 1e-74 51