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Name : Tpau_0523 (Tpau_0523) Accession : YP_003645503.1 Strain : Tsukamurella paurometabola DSM 20162 Genome accession: NC_014158 Putative virulence/resistance : Resistance Product : D-alanine--D-alanine ligase Function : - COG functional category : M : Cell wall/membrane/envelope biogenesis COG ID : COG1181 EC number : 6.3.2.4 Position : 552270 - 553304 bp Length : 1035 bp Strand : - Note : TIGRFAM: D-alanine/D-alanine ligase; KEGG: sco:SCO3595 D-alanine:D-lactate ligase; PFAM: D-alanine--D-alanine ligase domain protein; ATP-dependent carboxylate-amine ligase domain protein ATP- grasp; protein of unknown function DUF201 DNA sequence : ATGAGCAGGATCAGGATCGGCGTCCTCTTCGGTGGCGCCTCCGAGGAGCATCCCGTCTCCGTGAAGTCGGCCCGGGAGGT CGCGCGCAACCTCGATCCGGACCGGTACGAGGCGCACTGGATCGGCATCACCCGAGACGGTGCATGGCGCCTGTGCGCAG GGCCTGAGAACGACTGGGAGTCCGGCTCCGTCCCTGCGATGCTCTCCCCGGACCGCGGCGATCACGGGCTGCTGGTGCGC GAGGCCGGCAGGTACCGGGTGGTCGAGCTCGATGTGATCCTCCCGGTGCTGCACGGCCGGTTCGGCGAAGACGGTGCGGT GCAAGGGCTGTGCGAGCTCTCCGGTGTCGATTACATCGGCTGCGATGTGCCGAGCTCCGCCATCTGTATGGACAAGTCGC TCGCCTATGTGATCAGCTCCGCGGCCGGTATCGCGACCCCGGAGTTCCGGATCGTGGCGGGGCACGAAGCACCCGACCCC GCCGAGCTCGCCTACCCGGTGTTCGTCAAACCGGCCCGCTCCGGCTCGTCCTTCGGGGTGAGCAAGGTGACGGCCCCGGA CGATCTGCCCGCTGCCATCGAGGCGGCTCGGGAGTACGACTCGAAGGTGCTCATCGAACGGGCCGTCGCGGGCAGCGAGG TGGGCTGCGCGGTACTCGGCGATGGTCGCGGGCTCACGGTCGGCGAGGTCGACCACATCGCACTGTCGCACGGCTTCTTC CGGATCCACCAGGAGGACTCCCCCGAGACCGGATCGGAGAACTCGACACCGATCGTGCCCGCCGACATCCCGGCGGAATC CCGTGCCCGCGTGCAGCAGACCGCGCGCGCCATCTACCGGGCCCTCGGCTGCTCCGGCCTGGCCCGGGTGGACATGTTCC TCACCCCCGGCGGCGAGGTCGTCCTCAATGAGGTCAACACGCTGCCGGGTCTCACCTCATACAGCCGCTACCCCCGGATG ATGTCCGCCGCCGGGATATCCCTCGCCGAGGTGCTCGACCGCCTCGTCTGCCTCACTCGCACAGGAGGATCGTGA Protein sequence : MSRIRIGVLFGGASEEHPVSVKSAREVARNLDPDRYEAHWIGITRDGAWRLCAGPENDWESGSVPAMLSPDRGDHGLLVR EAGRYRVVELDVILPVLHGRFGEDGAVQGLCELSGVDYIGCDVPSSAICMDKSLAYVISSAAGIATPEFRIVAGHEAPDP AELAYPVFVKPARSGSSFGVSKVTAPDDLPAAIEAAREYDSKVLIERAVAGSEVGCAVLGDGRGLTVGEVDHIALSHGFF RIHQEDSPETGSENSTPIVPADIPAESRARVQQTARAIYRALGCSGLARVDMFLTPGGEVVLNEVNTLPGLTSYSRYPRM MSAAGISLAEVLDRLVCLTRTGGS |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| vanB | NP_815951.1 | D-alanine--D-lactate ligase | Not tested | Not named | Protein | 6e-107 | 61 |
| vanB | YP_008394259.1 | Vancomycin B-type resistance protein vanB, D-alanine--D-lactate ligase | Not tested | Not named | Protein | 3e-109 | 61 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| Tpau_0523 | YP_003645503.1 | D-alanine--D-alanine ligase | U00456.gene.p01 | Protein | 3e-107 | 61 |
| Tpau_0523 | YP_003645503.1 | D-alanine--D-alanine ligase | AF310956.2.orf5.gene | Protein | 3e-108 | 61 |
| Tpau_0523 | YP_003645503.1 | D-alanine--D-alanine ligase | U35369.1.gene6.p01 | Protein | 3e-107 | 61 |
| Tpau_0523 | YP_003645503.1 | D-alanine--D-alanine ligase | AE016830.1.gene2250. | Protein | 3e-107 | 61 |
| Tpau_0523 | YP_003645503.1 | D-alanine--D-alanine ligase | AY145441.gene.p01 | Protein | 2e-108 | 61 |
| Tpau_0523 | YP_003645503.1 | D-alanine--D-alanine ligase | NC_014475.1.orf3.gen | Protein | 1e-104 | 60 |
| Tpau_0523 | YP_003645503.1 | D-alanine--D-alanine ligase | NC_005054.2598280.p0 | Protein | 1e-104 | 60 |
| Tpau_0523 | YP_003645503.1 | D-alanine--D-alanine ligase | AF277571.gene.p01 | Protein | 2e-104 | 59 |
| Tpau_0523 | YP_003645503.1 | D-alanine--D-alanine ligase | AF130997.1.orf4.gene | Protein | 8e-104 | 59 |
| Tpau_0523 | YP_003645503.1 | D-alanine--D-alanine ligase | AY489045.gene.p01 | Protein | 8e-102 | 59 |
