Gene Information

Name : Tpau_0522 (Tpau_0522)
Accession : YP_003645502.1
Strain : Tsukamurella paurometabola DSM 20162
Genome accession: NC_014158
Putative virulence/resistance : Resistance
Product : D-Ala-D-Ala dipeptidase
Function : -
COG functional category : M : Cell wall/membrane/envelope biogenesis
COG ID : COG2173
EC number : 3.4.13.22
Position : 551638 - 552270 bp
Length : 633 bp
Strand : -
Note : KEGG: cai:Caci_5792 peptidase M15D VanX D-ala-D-ala dipeptidase; PFAM: peptidase M15D vanX D-ala-D-ala dipeptidase

DNA sequence :
ATGAACACCGACTTCGTCTACGTCGACGAGCACGTCCCGGGAGTTCGCTGGGACGCGAAATACGCCACCTGGGACAACTT
CACCGGCAAGCCCGTGGACGGCTACCTGGCCAACCGGATCGTGGGCACCCGCGTTCTGTGCGCCGGCCTCCGCCTGGCGC
AGCGGCACGCAGCCACCCTGGGATACGGATTGCTGCTGTGGGACGGGTACCGGCCGCAGCGCGCCGTCGACCGGTTCGTG
GCCTGGTCCCGACAGCCGGAGAACGGCAGGACCAAGCAGCGGCACTACCCGAACATCGCCCGCGCCGACATGTTCGAGCT
CGGCTATGTGGCCACCCGTTCCGGACACAGCCGGGGCAGCACCGTCGATCTCACCCTGTACCACCTGGATTCCGGCGACC
TCGCCGATATGGGCGGTGACCACGACCTGATGGATCCGGTCTCGCACCACGGTGCGCCCGGCATCGGCGAGCCCGCGGCC
CGCAACCGCGCCCGGCTGGCCACGATCATGGAGGACGCTGGATTTCTCCGCTACGACAGTGAATGGTGGCACTACACGCT
GCACGACGAACCCTTCCCCACCACCTACTTCGACTTCCCGATCACTCTTGCGGCGGGCAGCCGCGCCGCGTGA

Protein sequence :
MNTDFVYVDEHVPGVRWDAKYATWDNFTGKPVDGYLANRIVGTRVLCAGLRLAQRHAATLGYGLLLWDGYRPQRAVDRFV
AWSRQPENGRTKQRHYPNIARADMFELGYVATRSGHSRGSTVDLTLYHLDSGDLADMGGDHDLMDPVSHHGAPGIGEPAA
RNRARLATIMEDAGFLRYDSEWWHYTLHDEPFPTTYFDFPITLAAGSRAA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
vanX NP_815950.1 D-alanyl-D-alanine dipeptidase Not tested Not named Protein 3e-61 62
vanXB YP_008394260.1 D-alanyl-D-alanine dipeptidase Not tested Not named Protein 8e-61 61

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Tpau_0522 YP_003645502.1 D-Ala-D-Ala dipeptidase U82965.2.orf32.gene. Protein 2e-72 73
Tpau_0522 YP_003645502.1 D-Ala-D-Ala dipeptidase NC_014475.1.orf4.gen Protein 3e-59 62
Tpau_0522 YP_003645502.1 D-Ala-D-Ala dipeptidase AF130997.1.orf5.gene Protein 1e-61 62
Tpau_0522 YP_003645502.1 D-Ala-D-Ala dipeptidase NC_005054.2598281.p0 Protein 3e-59 62
Tpau_0522 YP_003645502.1 D-Ala-D-Ala dipeptidase U35369.1.gene7.p01 Protein 2e-61 62
Tpau_0522 YP_003645502.1 D-Ala-D-Ala dipeptidase AE016830.1.gene2249. Protein 1e-61 62
Tpau_0522 YP_003645502.1 D-Ala-D-Ala dipeptidase M97297.gene.p01 Protein 3e-59 62
Tpau_0522 YP_003645502.1 D-Ala-D-Ala dipeptidase AF155139.2.orf6.gene Protein 3e-61 62
Tpau_0522 YP_003645502.1 D-Ala-D-Ala dipeptidase AF310956.2.orf6.gene Protein 3e-61 61
Tpau_0522 YP_003645502.1 D-Ala-D-Ala dipeptidase FJ349556.1.orf5.gene Protein 1e-55 57