Gene Information

Name : Tpau_3685 (Tpau_3685)
Accession : YP_003648602.1
Strain : Tsukamurella paurometabola DSM 20162
Genome accession: NC_014158
Putative virulence/resistance : Virulence
Product : AMP-dependent synthetase and ligase
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 3795586 - 3797262 bp
Length : 1677 bp
Strand : -
Note : PFAM: AMP-dependent synthetase and ligase; KEGG: rop:ROP_48840 2,3-dihydroxybenzoate-AMP ligase

DNA sequence : 
ATGACCGCTGACAACGACCTCACGACCGAGTACTTCCCGGCCGATGTCGCGGCGCGCTACCGCGCCGCGGGGTACTGGAC
CGACGACACCTTCGCCGATTTCCTGCCCGCCGCGGCGGCCCGCTTCGGTGATGCGGAGGCACTGGTCGGACGGGACCACC
GCGGCGCCGCCGCGCGCCTGACCTACCGCGAGATCGACCGCGCGGCATCCGCCGTCGCCTCGGGTCTGCGGGCCCGTGGC
CTCGGGCCGGGCGATCGCGTCGTGGTGCAACTGCCCAATATCGTCGAGTTCGTCCCGCTGCTCTTCGGCGTCTTCCGGAT
CGGAGCGATCCCGGTCTTCGCGTTACCCGCGCATCGTCGCAGTGAACTCGTGCACTTCATCGAGACGTCGGGCGCGAAGG
CGTTGATCACCGCGGGCCGCGCAGCGGGCTTCGACCACCGCGACCTCGCCCGCGACGTGCTCGCCGAGCTCGACGGCCCG
GGCCCGCTGGTGATCGTCGCCGACGACGAGCCGGCGGAGTTCGTGGGCCTCGCCGACCTCCTGGAGGGTGGACGCGACGC
CGACGGCGCGCCCGCCGCGGTCGCCGCCGGCGCCCTCGCCTTCTTGCAACTCTCCGGCGGCACCACCGGTGTGCCCAAAC
TGATTCCGCGCACGCACGCCGACTACCTGTACTCGGTGCGCGAATCCGCGAAGATCTGCGGTCTAGGGCCCGACACGCGG
TTCCTCGTGGTGTTGCCCGTGGCGCACAACTTCCCGATGAGCTCGCCCGGCATCCTCGGCGTGCTGTGGGCCGGCGGCAC
CGTGGTGTTGGGCAGCGACCCCGCTCCGTACCGCGCCCTGCCGTTGATCGAGGCCGAGCGGATCACCATGACCTCGCTGG
TGCCGCCGGTCGCGATGCTGTGGCTGCAGGCCAGGCCCACCACCGATGCCGATCTGAGCAGCCTGCAGGTGTTGCAGGTG
GGCGGCGCCAAGTTCCCCGTGGAGGCGGCGCGCCGCGTGCAGCCCGAGCTCGGCTGCCGCCTGCAGCAGGTCTTCGGTAT
GGCTGAGGGCCTGGTCAACTACACCCGCGACGACGACCCCGACGACCTCGTGCTCGGCACGCAGGGCCGTCCCATCTCGC
CCGACGACGAGGTCCGGGTGGTCGATGACGACGGCGCACCGGTGCCCGCCGGTGCGGCGGGACACCTGCTGACCCGGGGC
CCGTACACGATTCGCGGCTACCTGGGCGGTGTGGACGCCGCCTCGTTCGACGCCGAGGGCTTCTACCGCACCGGCGACAT
CGTGCGCCGCCTGCCGAGCGGCCACCTGGTGGTCGAGGGCCGGGCGAAGGACCAGATCAACCGCGGCGGCGAGAAGATCG
CCGCCGAGGAAGTCGAGAATCACCTCGTCGCCCACCCGGCCGTGATCGACGCCGCCGTGGTGGCCGTCGCCGATGAATTT
CTCGGCGAGCGCACCTGCGCGGTGATCCTCGGCGCACCCGGCGAGGACCGCCCCACCGTCCCCCAACTCAAGAAGTTCGT
CCGCGAGCGCGGCGTGGCCGCGTACAAAGTGCCCGACCGGATCGAGTTCGTCGATGCGTTCCCCACCACCGGCGTCGGCA
AGATCAGCCGGCGCGAACTGCGGCGCGCACTCGCCGACCTGGTCGCCGCGCGCACCGATACCGAGGAGAAGAAGTGA

Protein sequence : 
MTADNDLTTEYFPADVAARYRAAGYWTDDTFADFLPAAAARFGDAEALVGRDHRGAAARLTYREIDRAASAVASGLRARG
LGPGDRVVVQLPNIVEFVPLLFGVFRIGAIPVFALPAHRRSELVHFIETSGAKALITAGRAAGFDHRDLARDVLAELDGP
GPLVIVADDEPAEFVGLADLLEGGRDADGAPAAVAAGALAFLQLSGGTTGVPKLIPRTHADYLYSVRESAKICGLGPDTR
FLVVLPVAHNFPMSSPGILGVLWAGGTVVLGSDPAPYRALPLIEAERITMTSLVPPVAMLWLQARPTTDADLSSLQVLQV
GGAKFPVEAARRVQPELGCRLQQVFGMAEGLVNYTRDDDPDDLVLGTQGRPISPDDEVRVVDDDGAPVPAGAAGHLLTRG
PYTIRGYLGGVDAASFDAEGFYRTGDIVRRLPSGHLVVEGRAKDQINRGGEKIAAEEVENHLVAHPAVIDAAVVAVADEF
LGERTCAVILGAPGEDRPTVPQLKKFVRERGVAAYKVPDRIEFVDAFPTTGVGKISRRELRRALADLVAARTDTEEKK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 7e-85 46
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 9e-85 46
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-86 46
ybtE CAA21394.1 - Virulence HPI Protein 5e-86 46
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 4e-86 46
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 6e-86 46
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-86 46
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 5e-86 46
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-86 46

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Tpau_3685 YP_003648602.1 AMP-dependent synthetase and ligase VFG0168 Protein 2e-108 52
Tpau_3685 YP_003648602.1 AMP-dependent synthetase and ligase VFG1817 Protein 2e-95 47
Tpau_3685 YP_003648602.1 AMP-dependent synthetase and ligase VFG0932 Protein 2e-93 46