Gene Information

Name : Tpau_2490 (Tpau_2490)
Accession : YP_003647433.1
Strain : Tsukamurella paurometabola DSM 20162
Genome accession: NC_014158
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 2583825 - 2585255 bp
Length : 1431 bp
Strand : -
Note : TIGRFAM: pyruvate kinase; KEGG: rha:RHA1_ro01007 pyruvate kinase; PFAM: pyruvate kinase barrel; pyruvate kinase alpha/beta

DNA sequence :
GTGTCAGCAGTGACTCGTCGAACCAAGATCGTCTGCACCCTCGGACCCGCCGTCGCCACCGATGAGAAGGTCCTTGCTCT
CGTCACGGAGGGCATGGACGTCGCGCGCCTCAACTTCAGCCACGGCGAACACGCCGACCACGAGGTCAACTACAACCGCG
TGCGCGCCGCCTCCGATACCACGGGCCGCGCCGTGGGAATCCTCGCCGACCTGCAGGGACCGAAGATCCGGCTGGGCCGG
TTCGCCGGCGACGGTCGCACGGTCTGGGAGACGGGTGAGACGGTGCGGATCACCGTCGACGACGTCGTGGGCACCCACGA
CCGGGTGTCGACCACCTACGCAGAGCTCGCGCAGGACGCCCGCCCCGGCGACCGGCTCCTCGTCGACGACGGCAAGGTGG
GGCTCAAGGTGCTGTCGGTGGACGGCAACGACGTCGTCTGCGAGGTCACCGAGGGCGGCCCGGTGTCGAACAACAAGGGC
GTCTCGCTGCCGGGGATGAACGTCTCCGTTCCGGCGCTGAGCGACAAGGACATCGAGGACCTCGAGTTCGCTCTCAAGCT
GGGCGTCGACTTCATCGCCCTCTCCTTCGTCCGTTCACCGGCTGATATCGAGCGAGTGCACGCCGTGATGGATCGCGTCG
GCCGCCGGGTACCGGTGATCGCCAAGCTCGAGAAGCCGGAGGCGATCGACAACCTCGAGGCCGTCATCCTGGCCTTCGAT
GCGGTGATGGTGGCCCGCGGCGACCTGGGCGTCGAGCTGCCGCTCGAAGAGGTGCCGCTGGTGCAGAAGCGCGCGATCCA
GATCGCCCGCGAAAACGCCAAGCCTGTCATCGTTGCGACCCAGATGCTGGAGTCGATGATCGAGAACAGCCGGCCCACTC
GGGCCGAGGCCTCCGACGTCGCGAACGCCGTGCTCGACGGTGCCGATGCCGTGATGCTCTCCGGCGAGGTGTCGGTGGGC
AAGTACCCGATCGAAACCGTGCAGACCATGGCCAAGATCGTGCGGGCGGTGGAGGAGGGCGGTCCCTCGGTGCCCCCGCT
CAACCACGTTCCCCGCACCAAGCGCGGCATCATCTCCTACGCCGCCCGCGATATCGGCGAGCGACTCAACGCCAAGGCCC
TCGTGGCCTTCACCCAGTCGGGCGACACCGTGCGCCGCCTGGCCCGGCTGCACACCCGGCTCCCGCTGCTGGCGTTCACC
CCGCTGCCGGAGGTGCGCAGTCAGCTCACTCTGTCCTGGGGTACCGAGACCTTCCTGGTCGACGGTGCCGACAGCACCGA
CGCGATGATCCACCAGGTGGATCAGTCGCTCGAAGGCATCGGCCGCTACTCCAAGGGCGACCAGGTGGTCATCGTCGCCG
GTGCGCCCCCCGGGACCATCGGCTCGACCAACCTGATCCAGGTGCACCGGATCGGCGAGGACGACCACTAG

Protein sequence :
MSAVTRRTKIVCTLGPAVATDEKVLALVTEGMDVARLNFSHGEHADHEVNYNRVRAASDTTGRAVGILADLQGPKIRLGR
FAGDGRTVWETGETVRITVDDVVGTHDRVSTTYAELAQDARPGDRLLVDDGKVGLKVLSVDGNDVVCEVTEGGPVSNNKG
VSLPGMNVSVPALSDKDIEDLEFALKLGVDFIALSFVRSPADIERVHAVMDRVGRRVPVIAKLEKPEAIDNLEAVILAFD
AVMVARGDLGVELPLEEVPLVQKRAIQIARENAKPVIVATQMLESMIENSRPTRAEASDVANAVLDGADAVMLSGEVSVG
KYPIETVQTMAKIVRAVEEGGPSVPPLNHVPRTKRGIISYAARDIGERLNAKALVAFTQSGDTVRRLARLHTRLPLLAFT
PLPEVRSQLTLSWGTETFLVDGADSTDAMIHQVDQSLEGIGRYSKGDQVVIVAGAPPGTIGSTNLIQVHRIGEDDH

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 3e-70 41
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 7e-70 41
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 4e-70 41
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 4e-70 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Tpau_2490 YP_003647433.1 pyruvate kinase VFG0479 Protein 2e-70 41