Gene Information

Name : Tpau_1737 (Tpau_1737)
Accession : YP_003646694.1
Strain : Tsukamurella paurometabola DSM 20162
Genome accession: NC_014158
Putative virulence/resistance : Unknown
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 1808615 - 1811977 bp
Length : 3363 bp
Strand : -
Note : TIGRFAM: lysyl-tRNA synthetase; KEGG: krh:KRH_21510 lysyl-tRNA synthetase; PFAM: tRNA synthetase class II (D K and N); protein of unknown function DUF472; protein of unknown function DUF471; protein of unknown function DUF470; nucleic acid binding OB-fold

DNA sequence :
ATGCAGCAGAGCGTCCGCGAGCGGACCACCGAGGCGACTGCCACCTGGCTGGCCCGGACGATGTTCTTCGCCGCCATCGT
CTCACTGCTGGGCGCGCTGGTCCACGGCGACCGCATCCTGCCGATCCTCGCCGATGTGCTGGGCGTGCTCGGCATCCCCT
CGTTCCCCAGCCTGCTGTTCGTCGCGCTGCTGACGGTGCTTAGCGGGGCGCTGCGCCGGCGGCTCCGGGCCGCGCACACC
GTGGCCTGCGCTCTCATGGCCACGGAGGTCGCGGCGAACATTTTCGTCCTGGTGCTCGTGGTCACCGAGGAGCTCGGCGA
CGACGACGGCAGCGCCCGCGTGCCGGCCCTGCTGGCGAACTACTTCGAGGCCCGCTACTCGTTGCCGGGCGCGGCCTTCG
CGGCTGCGGTGAGCGCGGCGACGCTCGCCCTGGTCATCGCCGCCCGGCCCGCCTTCCCCGCGAAGCTGGCCCACGGGTCC
CGCCGCGCGGCGCTCGGCGTCCTGGTGGGCGGGCTACTGCTGTCGTACCTGGTCACGCTGGCGCTCACGTTCACCTTCCC
GGGCCGGCTACGCGGTCCGGTCGAGATCGCGGTGTGGGCGCTGCGCTCGACCTTCGGCGTCTCACTCCCCCGCCCGCTGG
ACCTGCTGCTCAACGGTCACTCCGGCCCGCAGTGGATCTACTCCTTCGCCGGCATCATGTCGGCGACGGTGCTGATCCTG
GCGCTGATGGCGTTCTGGCGCGCCGGTCGCGGCGACGAGCTGATGACCGAACCGGAGGAACTGGAGGTGCGCCGCCTGCT
GCTCACCTACGGCGAGGACGATTCACTGGGCTACTTCGCCACCCGGCGCGATAAGTCGGTGATCGCGGCTCCCGATCGCA
GAGCGGTGGTCACCAGCCGGGTGGTGGGCCCGGTGTCGATGGCGAGCGCCGATCCGATCGGCGACCGCGAGTCGTGGCCC
GCCGCCGTGCACGCCTGGCTGGAGGACTGCCGCCGACACAGCCTGTACCCGGCGGTCCTTGCGGCGAGCATCGACGGTGC
CGCGGTGTACGAGGCTGCCGGGCTGCGGACACTCAACATCGGCGACGAGGCGATCATCCACGTCGATCGGTTCACGCTCA
AGGGGCGCGAGATGCGTGCGGTACGGCAAGCCGTCACCCGCGTGAGCGGCGCCGGGTACACGCTGCAGGTGCGCCGCCAC
GCGGACCTCACCACCGAGGAGCTGACGCAGGTCGCACAGCTCGCGGAGAAGTGGCGCGGCGAGGAGACCGAGCGCGGCTT
CTCCATGGCCCTCAACCGGATCGGCGACCCGGTGGACGGTCGGTGCGTGCTGGTGACCGCGCACGACGCCGCCGGCGAGG
TGCGCGGTCTTTTGAGCTTCGTGCCCTGGGGCACCCGCGGCCTCTCGCTCGATCTGATGCGTCGCGACCGGAACGCGGAG
AACGGGCTCAACGAGTTCATGGTGGCCAAACTCGTCGAGGCCAGCGCGGAGATCGGCATCCGTCGCATCTCCTTGAACTT
CGCCGTTTTCCGCGCTGTCTTCTTCGAAGCAGACCGGGTGGGCGCGGGGCCCGTCACCCGGATGGTGGATTCGGTGCTCA
GCTTCGCGTCGAAGTTCTATCAGTTGGAAACGTTGTATCGGTCGAACGACAAGTACCGACCGGACTGGGTGCCTCGGATC
CTGTGCTACGACCCGACGCTCACCGCGGTGCGAGCCGGTATCGCGGTGGGAACCGCCGAGGGCTTTCTGCCGCAGATCGG
GCCGAAGTTCCTCTCCGGCCCCACTGTTCCCGACGAGCAGGATCAGCGCGACCCAGAATTCGTCGTGCGCCTCCTCGCCG
AGGAGGAGCGCCTACTGCACCGCGAGGCGCCCGCACCACGGCTTTCCGAACAGCAGCGGGTGCGTCGCCGCAAACTCACG
GCGATGGAGGCGGCGGGGATGCCCGGCTATCCCCCCTCGGTGCCGCGCACCGCCTCGCTCGCCGAGGTTCGCGGGCACCT
CAATGGCCTGGCCCCCGATTCATCGACGGACACCGTTGTTTCCGTGACCGGCAGGGTGCGTGGCGTGCGTGACTACGGTG
GCGTCACCTTCGTCGATCTTCACGAGGACAGGGCCACCTTGCAGGTGATCGCCGAGCGCGGTCGTACTCCCGACGACGTG
CGCGCCTTCTGGCGCGAGTCGATCGACATGGGCGACCTGGTGTCGGTGACCGGTGAGATGGTCACTTCGCACACGGGTGA
GCTCTCGGTTCTGCTCCAGGACTGGGCCATGGCCGGGAAGTGCCTCTCACCGGTCCCGAAGCCGCGGGTGCGACTCGGCG
ACGAGACCAGGGCGCGCAGCCGCTCCCTCGACCTGATGACCGATGACGGTGCTGTCGACCTGCTCTACCGCCGCTCGCGG
GCGGTCGCGGCAATGCGCGCCGCGTTCACCGATCGCGGCTTCACCGAGGTCGAGACGCCGATGCTGCAGTCGGTGCACGG
CGGCGCGGCGGCACGCCCGTTCACCACGCACATCAACGCCTACGACATGGATCTGTACCTCCGGATCGCGCCGGAGCTCT
ACCTCAAGCGGCTCGCGGTGGGAGGCATGGGCCGGATCTTCGAGATCAACCGGAACTTCCGCAACGAGGGCGCCGACGCC
ACCCACAACCCGGAGTTCACCTCGCTCGAGGCCTACGAGGCGTACGGCGACTACACCACCATGCGAACACTGACCCGCGA
GGTGATCCTCGGCGCCGCGATCGCCGTCAACGGCCGGCCGATCGCGGTACGGCCCGACGGTTCCGGTGGAACACGCGAGG
TGGACCTCACCGCCGAATGGCCGGTGATCACGGTGCACGAGGCTGTTTCCCGGGCCGTCGGCACGTCGGTGACACCCGAC
AGCGAGGTCGAGGAGTTGGAGGCGCTGTGCAAGCAGTGGCACGTCGCGGCTCCCCGGGGAGCGTCGGCGGGCAAGCTGGT
CATGGAACTGTACGAGGGGCTGGTGGAAAAGCAGACCGAGTTCCCCACGTTCTACTGCGATTTCCCGATCGAGGTCTCGC
CACTGGCGCGCAGACACCGCCGCGATCCGCGACTCACGGAACAATGGGACCTGGTGGGTTTCGGCGCCGAGCTGGGTACT
GCCTACACGGAACTGACCGATCCGATCGATCAGCGCGAGCGGCTCACCCAGCAGTCGATGGCCGCGGCCGCCGGTGATCC
GGAGGCGATGGAACTCGATGAGTCCTTCCTGGATGCCCTGTCGTACGCCATGCCACCGACCGGCGGTCTCGGCATCGGTG
TGGACCGGATCATCATGCTGCTTGCGGGAGTGAACATCCGCGCCACGCTGGCCTTCCCGTTCATCAAACCCCAGGACCGC
TGA

Protein sequence :
MQQSVRERTTEATATWLARTMFFAAIVSLLGALVHGDRILPILADVLGVLGIPSFPSLLFVALLTVLSGALRRRLRAAHT
VACALMATEVAANIFVLVLVVTEELGDDDGSARVPALLANYFEARYSLPGAAFAAAVSAATLALVIAARPAFPAKLAHGS
RRAALGVLVGGLLLSYLVTLALTFTFPGRLRGPVEIAVWALRSTFGVSLPRPLDLLLNGHSGPQWIYSFAGIMSATVLIL
ALMAFWRAGRGDELMTEPEELEVRRLLLTYGEDDSLGYFATRRDKSVIAAPDRRAVVTSRVVGPVSMASADPIGDRESWP
AAVHAWLEDCRRHSLYPAVLAASIDGAAVYEAAGLRTLNIGDEAIIHVDRFTLKGREMRAVRQAVTRVSGAGYTLQVRRH
ADLTTEELTQVAQLAEKWRGEETERGFSMALNRIGDPVDGRCVLVTAHDAAGEVRGLLSFVPWGTRGLSLDLMRRDRNAE
NGLNEFMVAKLVEASAEIGIRRISLNFAVFRAVFFEADRVGAGPVTRMVDSVLSFASKFYQLETLYRSNDKYRPDWVPRI
LCYDPTLTAVRAGIAVGTAEGFLPQIGPKFLSGPTVPDEQDQRDPEFVVRLLAEEERLLHREAPAPRLSEQQRVRRRKLT
AMEAAGMPGYPPSVPRTASLAEVRGHLNGLAPDSSTDTVVSVTGRVRGVRDYGGVTFVDLHEDRATLQVIAERGRTPDDV
RAFWRESIDMGDLVSVTGEMVTSHTGELSVLLQDWAMAGKCLSPVPKPRVRLGDETRARSRSLDLMTDDGAVDLLYRRSR
AVAAMRAAFTDRGFTEVETPMLQSVHGGAAARPFTTHINAYDMDLYLRIAPELYLKRLAVGGMGRIFEINRNFRNEGADA
THNPEFTSLEAYEAYGDYTTMRTLTREVILGAAIAVNGRPIAVRPDGSGGTREVDLTAEWPVITVHEAVSRAVGTSVTPD
SEVEELEALCKQWHVAAPRGASAGKLVMELYEGLVEKQTEFPTFYCDFPIEVSPLARRHRRDPRLTEQWDLVGFGAELGT
AYTELTDPIDQRERLTQQSMAAAAGDPEAMELDESFLDALSYAMPPTGGLGIGVDRIIMLLAGVNIRATLAFPFIKPQDR

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
lysS1 YP_005682181.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 7e-170 48
lysS1 YP_005684273.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 7e-170 48
lysX YP_003784331.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 7e-170 48
lysS1 YP_005686365.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 7e-170 48