Gene Information

Name : Tint_1608 (Tint_1608)
Accession : YP_003643311.1
Strain : Thiomonas intermedia K12
Genome accession: NC_014153
Putative virulence/resistance : Resistance
Product : mercuric reductase
Function : -
COG functional category : C : Energy production and conversion
COG ID : COG1249
EC number : -
Position : 1721308 - 1722978 bp
Length : 1671 bp
Strand : +
Note : KEGG: net:Neut_2567 putative mercuric reductase; TIGRFAM: mercuric reductase; PFAM: FAD-dependent pyridine nucleotide-disulphide oxidoreductase; Heavy metal transport/detoxification protein; pyridine nucleotide-disulphide oxidoreductase dimerisation regio

DNA sequence :
ATGGAAATCCTGAGCATCAGCGGCATGACCTGCAACGCCTGCGCCGCCCACGTCAAAAGCACCCTGGAGAGCCTGCCGGG
CGTGAAATCCGCGCAGGTGTCGTACCCGCTGGGGACGGCCGAAGTCGTGGCCGATGACAGCATCTCGTTCGACGCGATGG
CTGCGGCCGTGGCCAAGGCGGGCTACGCGGCGAAGCAGGCGGATCGTGCGCCGACGCCGTCGTACGGTTCAACCGACTTG
GCCCGTTCTACCCATCCTGCCCGCGGTGACGCCAGCGGTCTGCGCATCGCCGTCATCGGCAGCGGCGGCGCGGCCATGGC
GGCGGCGATCAAGGCGGCCGACAGCGGCGCGCGCGTCACGCTGATCGAACGCGGGGTGATCGGCGGCACCTGCGTCAACG
TCGGCTGTGTGCCGTCCAAGATCATGATCCGCGCTGCGCACATCGCCCAGTTGCGCCGCCGCAGCCCGTTCGACGCGGGC
ATTCCACCCACCTCCCCCGCCATCCTGCGCGACAAACTGCTCGCGCAGCAGCAGGCGCGGGTGGACGAGCTGCGCCACGC
CAAGTATGAAAGCATTTTGGACGGGCAGCCCGCCATCGCGACCCTGCGCGGCGCCGCCCGGTTCCAGGACGCCCGAACGC
TGGCCGTGACCCTGCACGACGGCAGCTCACGCCAGGTGAGCTTCGATCGCTGCCTCATCGCTACCGGCGCCAGTCCGTCC
ATTCCTCCGATTGCGGGACTGAAGGGCACGCCGTACTGGACGTCGACCGAGGCGCTGGAGGCCGATACGATTCCGCCGCG
CCTGGCGGTCATCGGCTCTTCTGTCGTCGCGCTGGAGCTGGCCCAGGCCTACTCCCGCCTGGGCAGCCAGGTCACCCTGG
TCGCGCGCAACACCCTGCTGTCGCGCGACGATCCGGCCATCGGCGAGGCGTTGACCGCCGCACTGCGCGAAGAGGGCCTT
ACGGTGCTGACTCACGCCCAGGTCGGTGCGGTGGCCTACGCCGACGGCTGCTTCGCGCTGACCACGAACCAGGGCGAGAT
CCGCGCCGAGCGCCTGCTGGTGGCCACGGGCCGCGCGCCCAACACCGCGGCGCTCGATCTGCACGCTGCCGGCGTGCAAT
GCGATGCGCGCGGCGCCATCGTGGTCGATGACCATTTGCGCACCAATGTGCCGCACATTTACGCCGCGGGCGACTGCACC
GATCAGCCGCAATTCGTCTATGTGGCCGCAGCCGGGGGCACGCGTGCGGCCGTCAACATGACCGGTGGCGAGGCGACGCT
CGACCTCTCGGCCATGCCGTCCGTGGTGTTCACCGACCCGCAAGTGGCCACGGTGGGACTGAGCGAGGCGCAGGCGCAGC
GACAAGGCATCCAGACCGACAGCCGCACGCTGAGCCTGGACAACGTCCCCCGCGCCCTGGCCAACTTCGACACGCGCGGC
TTCATCAAGCTGGTCGCCGAGGCGCAAAGCGGGCGCCTGCTCGGCGTCCAGGCGGTGGCCGCCGAGGCAGGCGAACTCAT
CCAGACGGCCGCGCTCGCCATTCGCAACCGCATGACGGTGCAGCAACTGGCCGACCAACTCTTCCCCTACCTGACCATGG
TCGAAGGCCTGAAGCTGGCCGCCCAGACCTTCACCAAGGATGTGACGCAACTGTCATGCTGCGCGGGGTGA

Protein sequence :
MEILSISGMTCNACAAHVKSTLESLPGVKSAQVSYPLGTAEVVADDSISFDAMAAAVAKAGYAAKQADRAPTPSYGSTDL
ARSTHPARGDASGLRIAVIGSGGAAMAAAIKAADSGARVTLIERGVIGGTCVNVGCVPSKIMIRAAHIAQLRRRSPFDAG
IPPTSPAILRDKLLAQQQARVDELRHAKYESILDGQPAIATLRGAARFQDARTLAVTLHDGSSRQVSFDRCLIATGASPS
IPPIAGLKGTPYWTSTEALEADTIPPRLAVIGSSVVALELAQAYSRLGSQVTLVARNTLLSRDDPAIGEALTAALREEGL
TVLTHAQVGAVAYADGCFALTTNQGEIRAERLLVATGRAPNTAALDLHAAGVQCDARGAIVVDDHLRTNVPHIYAAGDCT
DQPQFVYVAAAGGTRAAVNMTGGEATLDLSAMPSVVFTDPQVATVGLSEAQAQRQGIQTDSRTLSLDNVPRALANFDTRG
FIKLVAEAQSGRLLGVQAVAAEAGELIQTAALAIRNRMTVQQLADQLFPYLTMVEGLKLAAQTFTKDVTQLSCCAG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
merA ABQ57371.1 MerA Not tested SGI1 Protein 2e-171 71
merA AGK07021.1 MerA Not tested SGI1 Protein 2e-171 71
merA AGK07079.1 MerA Not tested SGI1 Protein 2e-171 71
merA AET25397.1 MerA Not tested PAGI-2(C) Protein 4e-170 70
merA AFG30120.1 MerA Not tested PAGI-2 Protein 4e-170 70
merA YP_006098387.1 mercuric ion reductase Not tested Tn2411 Protein 6e-170 70
merA ACN81005.1 MerA Hg(II) mercuric ion reductase Not tested AbaR5 Protein 6e-174 70
merA AAN62178.1 mercuric (Hg(II)) reductase Not tested PAGI-2(C) Protein 8e-172 70
merA CAJ77060.1 Mercuric ion reductase Not tested AbaR1 Protein 3e-174 70
unnamed ABR13401.1 mercuric reductase Not tested PAGI-5 Protein 3e-167 68
SE0085 NP_763640.1 dihydrolipoamide dehydrogenase Not tested SCCpbp4 Protein 6e-86 44
merA BAB47642.1 mercuric reductase Not tested Type-III SCCmec Protein 5e-86 44

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Tint_1608 YP_003643311.1 mercuric reductase BAC0652 Protein 1e-161 76
Tint_1608 YP_003643311.1 mercuric reductase BAC0653 Protein 7e-169 70
Tint_1608 YP_003643311.1 mercuric reductase BAC0650 Protein 6e-164 65
Tint_1608 YP_003643311.1 mercuric reductase BAC0224 Protein 3e-87 46
Tint_1608 YP_003643311.1 mercuric reductase BAC0651 Protein 3e-86 44