Gene Information

Name : Tint_1218 (Tint_1218)
Accession : YP_003642936.1
Strain : Thiomonas intermedia K12
Genome accession: NC_014153
Putative virulence/resistance : Virulence
Product : flagellar protein export ATPase FliI
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : -
Position : 1298542 - 1299888 bp
Length : 1347 bp
Strand : +
Note : TIGRFAM: flagellar protein export ATPase FliI; ATPase, FliI/YscN family; PFAM: H+transporting two-sector ATPase alpha/beta subunit central region; H+transporting two-sector ATPase alpha/beta subunit domain protein; KEGG: aeh:Mlg_0712 flagellar protein exp

DNA sequence :
ATGAGTCTGGCCCGGGTATCGCGCCATCTGCAGACTTTGCAACTTCGTCTGCAATCGGGCGGGCCTTTGCCTCTGGTCTA
TAGCGGCAGCCTGGTGCGGGTGCGGGGGCTTATTCTTGAAGCGCAGGGGCTGGAGGCCAGTCTGGGCGAGCGTTGTCGCA
TCAGCACCGAGCAGAACCGTGTCATCGACGCCGAAGTGGTGGGTTTCAACGGCGATCATGTGCAGCTCATGGCGCTGGGC
GAAGTGCAGGGCCTCACGCCGGGGGCGCGGGTGCATCCCGTGCCGGGCGATATTCGCATTCCCGTCGGGCCCGAGCTGCT
CGGGCGCGTGCTCGACGGCAACGGCCGTCCGCTCGACGGCGACGGCGCGCTGCTGGCCTCACACTACACCACCTTGTCCA
CCCAGCAGATCAACCCGCTGGGCCGGGCGCTGGTGCGCCAGCCGCTCGACGTGGGCGTGCGCGCGATCAATGCGCTGTTC
ACCGTGGGCCGTGGCGCGCGCATGGGGTTGTTCGCGGGCAGCGGCGTGGGCAAGAGCGTGCTGCTGGGCATGATGGCGCG
CAACACCAATGCCGACGTCATCGTGGTGGGCCTCATCGGCGAGCGCGGCCGCGAGGTCAAAGAGTTCATCGAAGACATTC
TGGGCGTGGAAGGGCGCAGCAGGGCCGTCGTGGTGGCCGCCCCGGCCGATGCTCCGGCGCTGACCCGCATTCGCGGCGCG
CGTCTGGCCACCGCGATGGCCGAGTATTACCGCGACCAGGGGCTGAACGTGCTGCTGCTGATGGATTCGGTCACCCGCTA
CGCCCTGGCGCTGCGCGAGGTGGCGCTGGCCGCAGGAGAGCCCCCGGCGGCGCGCGGTTTTCCGCCCTCGGTGTTCGCCA
AGCTGCCGGCACTGGTGGAGCGCGCGGGCAACGGGCCGGAGGGCGGCGGCAGCATCACCGCGTTCTACACCGTGCTGATG
GAAGGCGACGACCAGCAAGACCCGGTCGCCGACAATGTGCGCGCCATTCTCGACGGCCACATCGTGCTCAGTCGCCGTCT
GGCCGAGCAGGGGCACTTTCCGGCCATCGACCTGCAGGCGTCGATCAGCCGCGTGATGCCGGCCATCGCCACGCCGCTGG
AACTTCGGCGCATGCAGAAGCTCAAGGAAATGCAATCGCGCTACGCCAGCCAGCAAGACCTCATCGCCGTGGGCGCCTAC
ACCCCGGGCGCCGACCTGCAACTCGACCAGGCGGTGCTGGCGCACGTCGGCATCCGCGAGTTTTTGATTCAGGACATGCA
CGCCGCTGTCGGTCTGACACAGAGCCGACAGGAACTGGCCGATCTGATGACGCCCTACCTTGTTTGA

Protein sequence :
MSLARVSRHLQTLQLRLQSGGPLPLVYSGSLVRVRGLILEAQGLEASLGERCRISTEQNRVIDAEVVGFNGDHVQLMALG
EVQGLTPGARVHPVPGDIRIPVGPELLGRVLDGNGRPLDGDGALLASHYTTLSTQQINPLGRALVRQPLDVGVRAINALF
TVGRGARMGLFAGSGVGKSVLLGMMARNTNADVIVVGLIGERGREVKEFIEDILGVEGRSRAVVVAAPADAPALTRIRGA
RLATAMAEYYRDQGLNVLLLMDSVTRYALALREVALAAGEPPAARGFPPSVFAKLPALVERAGNGPEGGGSITAFYTVLM
EGDDQQDPVADNVRAILDGHIVLSRRLAEQGHFPAIDLQASISRVMPAIATPLELRRMQKLKEMQSRYASQQDLIAVGAY
TPGADLQLDQAVLAHVGIREFLIQDMHAAVGLTQSRQELADLMTPYLV

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 2e-86 50
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 5e-83 50
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-83 50
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 4e-83 50
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-81 49
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-81 49
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 8e-84 48
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 8e-84 48
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-83 48
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 8e-84 48
lscN AAO18044.1 LscN Virulence TTSS locus Protein 6e-76 48
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 3e-69 47
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 9e-69 47
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 5e-69 47
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 3e-69 47
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-69 47
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 1e-68 47
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-71 47
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-71 47
escN AAC38383.1 EscN Virulence LEE Protein 2e-74 46
escN AAC31513.1 L0034 Virulence LEE Protein 9e-75 46
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 9e-75 46
escN CAI43874.1 EscN protein Virulence LEE Protein 7e-75 46
escN YP_003236088.1 translocator EscN Virulence LEE Protein 2e-74 46
escN NP_290268.1 hypothetical protein Virulence LEE Protein 1e-74 46
ECs4568 NP_312595.1 EscN Virulence LEE Protein 1e-74 46
escN YP_003223475.1 translocator EscN Virulence LEE Protein 9e-75 46
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 1e-68 46
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 1e-68 46
unnamed AAL06369.1 EscN Virulence LEE Protein 7e-75 46
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 1e-66 45
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 2e-74 45
escN AAK26715.1 EscN Virulence LEE Protein 6e-74 45
escN AAL57542.1 EscN Virulence LEE Protein 6e-74 45
escN CAC81862.1 EscN protein Virulence LEE II Protein 2e-74 45
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 3e-67 45
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 4e-65 44
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 4e-65 44
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 1e-67 43
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 6e-72 43
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 3e-68 43
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 3e-68 43
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 3e-68 43
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 3e-68 43
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 1e-66 42
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 6e-67 42
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 3e-67 42
invC AAS66861.1 InvC Not tested SSR-2 Protein 5e-66 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Tint_1218 YP_003642936.1 flagellar protein export ATPase FliI VFG1254 Protein 1e-115 60
Tint_1218 YP_003642936.1 flagellar protein export ATPase FliI VFG2331 Protein 2e-104 59
Tint_1218 YP_003642936.1 flagellar protein export ATPase FliI VFG2499 Protein 3e-110 59
Tint_1218 YP_003642936.1 flagellar protein export ATPase FliI VFG2144 Protein 1e-78 47
Tint_1218 YP_003642936.1 flagellar protein export ATPase FliI VFG0390 Protein 3e-77 47
Tint_1218 YP_003642936.1 flagellar protein export ATPase FliI VFG0515 Protein 5e-69 47
Tint_1218 YP_003642936.1 flagellar protein export ATPase FliI VFG0192 Protein 2e-81 46
Tint_1218 YP_003642936.1 flagellar protein export ATPase FliI VFG0048 Protein 1e-81 46
Tint_1218 YP_003642936.1 flagellar protein export ATPase FliI VFG0730 Protein 9e-75 46
Tint_1218 YP_003642936.1 flagellar protein export ATPase FliI VFG0812 Protein 5e-75 46
Tint_1218 YP_003642936.1 flagellar protein export ATPase FliI VFG0555 Protein 1e-68 43
Tint_1218 YP_003642936.1 flagellar protein export ATPase FliI VFG1778 Protein 9e-67 43