Gene Information

Name : Bmur_0462 (Bmur_0462)
Accession : YP_003632764.1
Strain : Brachyspira murdochii DSM 12563
Genome accession: NC_014150
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 567267 - 568700 bp
Length : 1434 bp
Strand : -
Note : TIGRFAM: pyruvate kinase; KEGG: bhy:BHWA1_00011 pyruvate kinase; PFAM: pyruvate kinase barrel; pyruvate kinase alpha/beta

DNA sequence :
ATGAGGAAAACTAAAATAATAGCTACTTTAGGACCAAGATGGTCTAGCGAAGATATGATAAAAAAGATTATAGAAAACGG
GGCAGATGTATGCAGATTAAATTTTTCATTTGGGGCTTATGAAGAACATTTAGAGAGAATCAATATAATAAGAAAAGTTT
CTAAAGAATTGAGAAAACCGGTTGCAATACTAGCTGACATGCAGGGACCAAAAATAAGAATAGGCAAATTGAAAGAACCT
ATAGCAGTAAAAGAGGGCGATATCGTAAAATTAAGCGGATATAAAGAAACTAATGATGACAGCATTATTCCTACAACACA
TTCTAATATAGCACATGATGTAAAAAATGGCTCTATGCTTTTAATAGCTGACGGTACCATACAGCTTATAGTAGAATCAA
GCGATGAGGCTTCAAAACTTGTTGTATGTAAAGTTATCACTGGAGGTACTATATTAAGCAGCAAAGGCATTAATTTACCG
GGGGCCCATGTTACTACAGAAGTTTTAACAGAAAAAGATATTAATGATGCATTATTCTGTGCAAAAAATGGTGTAAATTA
TTTGGGTATGAGTTTTGTTAGGCGTGCAGAGGATGTTATAAGGCTTAGAAAAATTTTAGATGATAATAATTTTCAGCATG
TTGCTATAGTATCAAAAATAGAGAATACAGAATCTTTAGAAAATTTGGAAGAGATAATCAAACATTCTGACGGAGTTATG
GTAGCAAGAGGTGATTTGGGAGTAGAAATACCATTCGGAGAGGTTCCTGTTTGGCAGAAAAAGATATTAAAAATGGCCAA
TGATTTAGGTAAAATTACTATAATAGCTACTCAAATGCTTGAAAGTATGACAAAAAGTCCTGTACCTTCAAGAGCTGAAG
CCAGCGATGTTGCTAATGCTGTATTAGATGGTACTGATGTTGTTATGATGTCTGCTGAAACTGCTTCTGGTGATTATCCT
ATAGAATCAGTTAAAGCTATGGTTTCTATTGTAGAGGCTGCTGAGAGATCTATGATAAAAATATCTCATGATAATATGAA
TTTATTAGACAGAGGACTAAATGATGCATTAGCTGATAGTGCTTCTTATTTGTCATACAGTTTGAATAATAAAGTAATAA
TTGCACTTTCTAGGTCTGGAAGAACTGTAAAAAACTTATCTAAAAAAAGACCAAAAACTCCTATAGTATTTATGTCAGCT
GATTTTAATTTATGTAACAGCCTTTCTATATGCCATAATGTTTATACTATACTTATGCCTGAAGATTTAAACTTCAGTTC
TGGAATTAGTCATAGCGGACAGATAGATATACTTGAAGATACTATAGTTAAGCATAATTTAGCAAATCATGGTGATACTG
CAATAGTTGTCAGCGGAAGTAAGTGGCAGGGAAGATGGCAGGAAAATAGTGTTCGTGTAGTTATTATACACTAA

Protein sequence :
MRKTKIIATLGPRWSSEDMIKKIIENGADVCRLNFSFGAYEEHLERINIIRKVSKELRKPVAILADMQGPKIRIGKLKEP
IAVKEGDIVKLSGYKETNDDSIIPTTHSNIAHDVKNGSMLLIADGTIQLIVESSDEASKLVVCKVITGGTILSSKGINLP
GAHVTTEVLTEKDINDALFCAKNGVNYLGMSFVRRAEDVIRLRKILDDNNFQHVAIVSKIENTESLENLEEIIKHSDGVM
VARGDLGVEIPFGEVPVWQKKILKMANDLGKITIIATQMLESMTKSPVPSRAEASDVANAVLDGTDVVMMSAETASGDYP
IESVKAMVSIVEAAERSMIKISHDNMNLLDRGLNDALADSASYLSYSLNNKVIIALSRSGRTVKNLSKKRPKTPIVFMSA
DFNLCNSLSICHNVYTILMPEDLNFSSGISHSGQIDILEDTIVKHNLANHGDTAIVVSGSKWQGRWQENSVRVVIIH

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 3e-63 44
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 2e-63 44
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 2e-63 44
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 8e-63 43

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Bmur_0462 YP_003632764.1 pyruvate kinase VFG0479 Protein 1e-63 44