Gene Information

Name : cadA (BMD_3164)
Accession : YP_003598354.1
Strain : Bacillus megaterium DSM 319
Genome accession: NC_014103
Putative virulence/resistance : Resistance
Product : cadmium-translocating P-type ATPase
Function : -
COG functional category : P : Inorganic ion transport and metabolism
COG ID : COG2217
EC number : 3.6.3.3
Position : 3111047 - 3113608 bp
Length : 2562 bp
Strand : +
Note : -

DNA sequence :
ATGAAACACGAAGATAAAAAAAGTCATCAGCATACCGGAAGCTGCTGCAGCGGATCTTCTTCAGTAGAAATTGAAAGCAA
GACCTGCTGCTCCTCTTCTTCAGAAGCCGCATCTACTGAAGAGCCTTCTTCCTGTGGCACAAAGAGTCATGAACACAGTC
ATAGCTGCTGTTCAGATACGATTGCGTTAACGACCGATAAAGGCCAGCATTCTTGCTGCAGTGATCACGATATAGTACCA
GGCGACAGTGACATAGAAGAAACGGCTGAATACCGCGTATATGGAATGGATTGCCCTTCCTGCGCCGCGACGATTGAAAA
AAGTTTAAAAACATTAAACGATATTCAACACGTAACTCTCAACTATAATACAGGAAAGCTTCAGGTAGCGGCGGCAAGTG
AGTCAGCTCTGTCACTCATTCCAAGCTCAGTAGAAAAACTAGGGTTTAACATTGAGGCTATCCAATCAAATAAAAAAATG
AAAACGTATTTGATTGAAGGAATGGACTGCGCAGCTTGTGCGAACACGATTGTGAATCATTTAAAAACAGTTCCTGCTGT
CAAAGATGTACGCGTTAACTTTTCAACGGGAAAAGCACAAATTGAGCACGATAACGAAGCCAATGACATTATTAAAGAAG
TATCTAAAGCTGGGTATACCGCTACTTTAGTAACTAGCAGCAGGCAGTCTGCTGAGTCGCGGCACCATAAAGGGCAAAAT
GGACCGATTGTTTTTTCGGGTATTTTCATCGCATTAGGTTTTATCGGTTCTCATACAGGAATTGCTTCTTATATGACAAC
TGTACTCTACGCTATTGCCATGATTGTCAGCGGATATAAGCCGGCTAAAAGTGCTTATTACGGAATTAAAAGCCGTTCTT
TAGACATGAATGTTTTAATGACCGTTGCAGCACTGGGAGCAGCCGTTATTGGTGAATGGCTTGAAGGTGCTACGGTCGTT
TGGCTATTTGCTTTAGGTGTTGCTCTACAAACAAGGTCGATTGAACAAACAAGAAACTCGATACGAGGTTTGATGGACTT
AGCTCCTTCTGAAGCATGGGTGAAAGAAAACGGCCAGCTGATTAAAAAAGCAGCGGGAGATATTTCAATCGGCACTACGA
TTGTCGTAAAGCCAGGAGACCGAATTCCGCTAGATGGTGAAATTATAAACGGCGAATCCAGTATTAACCAAGCTCCTATT
ACAGGTGAATCTATTCCAGTTGATAAAGTAATCGGTGATACGGTTTATGCAGGCACCATTAACGAAAACGGATCGTTAGA
AATTAAGGTAACAAAGCTAGTGGAAGATACGACGATTTCTAAAATTATTCATTTAGTGGAAGAAGCACAGGAGAAAAAAG
CGCCTACTCAAGCATTTGTTGATAAATTTGCAACCATTTACACGCCTATCGTCTTTATTTTAGCTTTATTCATTATGGTT
ATCCCTCCCCTGTTTGATGGTGCATGGAGTGAGTGGTTTTACAAGGGGCTTGAATTATTAGTTGTGGCTTGTCCATGTGC
GCTAGTCATTTCTACTCCCGTCGCCATTGTATCTGCGATTGGAAACGCGGCGAAAAATGGCGTGCTCATTAAAGGCGGGA
CGTTTTTAGAAAAAGCTGGTGCAATCAATGCGATTGCTTTTGATAAAACAGGAACGCTAACAGAAGGCAAACCAGCCGTT
TCAGAGATTGTTTCCCTTGCTGCTGAAGAAAATCAGCTTTTAGCGATAACAAAAACGCTAGAAGACTATTCGAATCATCC
CATCGCCCGGGCTATCGTGGATTATGCAGCGGAGAAAAAAGTCGTTTCTTTACAAGGGTCAAATTATAAAATTCTTACAG
GAAAAGGCGTGCAGGCAACAATTAAAGATACGGTTTATTACGCAGGTAATGCAAAATTATTCTCAGATTTAGAAACACCT
CTATCCCACTGCTGGTCTCATATTGAAAAGCTTCAAAACGAAGGTAAAACGATTATTATTATCGGAACAGCTAAAAGTGT
TCTAGGCATTATTAGTGTAGCTGATACCATCAGACATACAACCGTATCTGCTTTAGAAAGCCTCAAGCAAAACGGAATGC
AGCAAATTGTGATGTTAACCGGTGATAACGAAGGAACAGCTAAAATGATTGCTTCTCAGTCTCGGGTAGACCGCTATTTC
GCCGATTTGCTGCCTGAAGACAAAGTGAAAGCCATACAGCAGCTTCAAGATGAAGGGTATCAAACGGCTATGGTTGGAGA
TGGAATTAATGATGCCCCTGCCCTTGCAACTGCTGATCTTGGCATTGCAATGGGCGGTGCTGGTACTGATACGGCCATGG
AAACAGCTGATATTGTTCTCATGGCAGACAATTTAGAAAAGCTTCCTCATACAATGAAGCTAAGCCGCAAAGCGTTAGCT
GTTATAAAACAAAACATTTGGTTTTCTATTATTGTTAAAGTCATTGCCTTAGCCTTTATCTTCCCAGGTTGGCTTACTTT
ATGGATTGCCGTCTTAAGCGATACGGGAGCAGCGCTACTTGTTATTTTAAACAGCCTTCGCTTACTCAAAATGAAAAATT
AA

Protein sequence :
MKHEDKKSHQHTGSCCSGSSSVEIESKTCCSSSSEAASTEEPSSCGTKSHEHSHSCCSDTIALTTDKGQHSCCSDHDIVP
GDSDIEETAEYRVYGMDCPSCAATIEKSLKTLNDIQHVTLNYNTGKLQVAAASESALSLIPSSVEKLGFNIEAIQSNKKM
KTYLIEGMDCAACANTIVNHLKTVPAVKDVRVNFSTGKAQIEHDNEANDIIKEVSKAGYTATLVTSSRQSAESRHHKGQN
GPIVFSGIFIALGFIGSHTGIASYMTTVLYAIAMIVSGYKPAKSAYYGIKSRSLDMNVLMTVAALGAAVIGEWLEGATVV
WLFALGVALQTRSIEQTRNSIRGLMDLAPSEAWVKENGQLIKKAAGDISIGTTIVVKPGDRIPLDGEIINGESSINQAPI
TGESIPVDKVIGDTVYAGTINENGSLEIKVTKLVEDTTISKIIHLVEEAQEKKAPTQAFVDKFATIYTPIVFILALFIMV
IPPLFDGAWSEWFYKGLELLVVACPCALVISTPVAIVSAIGNAAKNGVLIKGGTFLEKAGAINAIAFDKTGTLTEGKPAV
SEIVSLAAEENQLLAITKTLEDYSNHPIARAIVDYAAEKKVVSLQGSNYKILTGKGVQATIKDTVYYAGNAKLFSDLETP
LSHCWSHIEKLQNEGKTIIIIGTAKSVLGIISVADTIRHTTVSALESLKQNGMQQIVMLTGDNEGTAKMIASQSRVDRYF
ADLLPEDKVKAIQQLQDEGYQTAMVGDGINDAPALATADLGIAMGGAGTDTAMETADIVLMADNLEKLPHTMKLSRKALA
VIKQNIWFSIIVKVIALAFIFPGWLTLWIAVLSDTGAALLVILNSLRLLKMKN

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
cadA BAC57487.1 Probable cadmium-transporting ATPase (Cadmium efflux ATPase) Not tested Type-IIIinv SCCmec Protein 2e-170 46
unnamed BAB47609.1 cadmium resistance protein B Not tested Type-III SCCmec Protein 2e-170 46
SE0075 NP_763630.1 cadmium resistance protein B Not tested SCCpbp4 Protein 2e-166 45

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
cadA YP_003598354.1 cadmium-translocating P-type ATPase BAC0092 Protein 7e-101 43