Gene Information

Name : Btus_2787 (Btus_2787)
Accession : YP_003590576.1
Strain : Kyrpidia tusciae DSM 2912
Genome accession: NC_014098
Putative virulence/resistance : Resistance
Product : mercuric reductase
Function : -
COG functional category : C : Energy production and conversion
COG ID : COG1249
EC number : -
Position : 2857934 - 2859592 bp
Length : 1659 bp
Strand : -
Note : KEGG: gka:GK3096 mercuric reductase; TIGRFAM: mercuric reductase; PFAM: FAD-dependent pyridine nucleotide-disulphide oxidoreductase; Heavy metal transport/detoxification protein; pyridine nucleotide-disulphide oxidoreductase dimerisation region

DNA sequence :
ATGAGCAACAAATTGCAAGTGCGACTTGCGGTACGGGGAATGACCTGTACAGACTGCGAAGAACACGTGAAGAAAGCCTT
GCACAGTGCCGGGGCTGAGAAGATATCTGTCAATTTTCGCCGCGGCGAGGCGACATTTGAGATCAGTGATGCGTCCGGCA
TTGAAGCAGCAAAACGGGCCGTGGCCGACAGCGGTTATGAACCCAGTAACGCAGAGATTCTCCATCCTTTGAGAAGTGAT
TCCACATTCCATCGAACAAGTGGCGACGACTATGACCTTCTCATCATCGGGTCCGGTGGTGCTGCATTCTCGGCAGCGAT
TGAGGCGGTTTCTCGTGGTGCAAAAGTCGGCATGATTGAACGCGGTACGATTGGGGGAACCTGCGTGAACACCGGCTGCG
TGCCGTCGAAGACCATGCTTCGAGCCAGCGAAATCCACCACCTGGCCGCACAGCATCCATTCCCGGGACTTGAAACGTCG
GCCGGCCCGGTGAATCTTCACCAGCTCGTAGGAGCGAAGGACGAACTCGTGGCGGAACTACGGCAGCACAAGTATATCGA
TCTCATCGACGAGTATGGCTTCGATCTCATACGTGGCGAGGCTCGCTTTGCGGACCCGTCGACCATCGCGGTCGCCGGCC
GTATGATTCGGGCCGGCCGTTATCTCGTTGCCACGGGGGCCTCACCCGCCGTGCCGGACATTCCGGGGCTTGCGGATGTG
GACTACCTCGTCAGTACCGCGGCACTGGAGTTGACAGAGGTTCCGCAGCGTCTGGCCGTGATCGGATCCGGCTATATTGG
AATGGAACTCGGGCAAATGTTTCACCGCTTGGGTTCAGAGGTGACGTTGATGCAGCGAAGTGGCCGACTGCTTCGAGCCT
ATGAGCCAGAGGTGTCCGAAGCCGTCTCTCTCGCCTTGGCCTTTGAGGGAATCCATATCCTGACGGGTGTCACCTATGAG
CGGGTGGAACAACACGGGGATCTGAAGCGGATTTATATTACTGTGGACGGGCAGCAGCGGGTCGTGGAGGCCGATGCGCT
GCTGGTGGCCACGGGGCGAACACCGAACACGGCGGCGTTGAACTTGGAGGCGGCCCAGGTGCGTGTCGGCAGCCGGGGAG
AGGTGTTGGTGAACGAGTACCTGCAGACGAGCAACCCAAACGTGTATGCCGCAGGAGATGTGACACTGGGGCCGCAATTC
GTGTACGTAGCGGCGTATGAGGGAGCGATTGCGGCTGAAAACGCTCTGAGCGGCGAGCGGCGCAAGGTGGATCTCTCAGT
CGTGCCGGCGGTGACCTTCACGTCGCCCTCGATCGCCACGGTGGGGATGACGGAGGCGAGAGCGAGGTCGAAAGGCTATG
AAGTGATCACCTCGGTCTTACCACTCGATGCCGTGCCACGAGCGCTGGTCAACCATGACACGGCGGGGGTGATTAAGTTG
GTGGCCGACGGCAGAAGCCGAAAGTTGCTCGGTGTCCACGTCGTGGCGGACAACGCCGGGGATGTGATTTACGCCGGTGT
GCTCGCTGTAAAATTTGGACTCACGATCGAAGATTTGACATCCACTTTGGCGCCGTACCTGACCATGGCAGAAGGGTTGA
AACTGGCGGCGCTGACTTTCGACCGTGACGTAGCGAAATTATCTTGCTGTGCCGGATAA

Protein sequence :
MSNKLQVRLAVRGMTCTDCEEHVKKALHSAGAEKISVNFRRGEATFEISDASGIEAAKRAVADSGYEPSNAEILHPLRSD
STFHRTSGDDYDLLIIGSGGAAFSAAIEAVSRGAKVGMIERGTIGGTCVNTGCVPSKTMLRASEIHHLAAQHPFPGLETS
AGPVNLHQLVGAKDELVAELRQHKYIDLIDEYGFDLIRGEARFADPSTIAVAGRMIRAGRYLVATGASPAVPDIPGLADV
DYLVSTAALELTEVPQRLAVIGSGYIGMELGQMFHRLGSEVTLMQRSGRLLRAYEPEVSEAVSLALAFEGIHILTGVTYE
RVEQHGDLKRIYITVDGQQRVVEADALLVATGRTPNTAALNLEAAQVRVGSRGEVLVNEYLQTSNPNVYAAGDVTLGPQF
VYVAAYEGAIAAENALSGERRKVDLSVVPAVTFTSPSIATVGMTEARARSKGYEVITSVLPLDAVPRALVNHDTAGVIKL
VADGRSRKLLGVHVVADNAGDVIYAGVLAVKFGLTIEDLTSTLAPYLTMAEGLKLAALTFDRDVAKLSCCAG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
SE0085 NP_763640.1 dihydrolipoamide dehydrogenase Not tested SCCpbp4 Protein 2e-173 67
merA BAB47642.1 mercuric reductase Not tested Type-III SCCmec Protein 2e-173 67
merA ACN81005.1 MerA Hg(II) mercuric ion reductase Not tested AbaR5 Protein 2e-90 46
merA CAJ77060.1 Mercuric ion reductase Not tested AbaR1 Protein 1e-90 46
merA ABQ57371.1 MerA Not tested SGI1 Protein 1e-89 45
merA AGK07021.1 MerA Not tested SGI1 Protein 1e-89 45
merA AGK07079.1 MerA Not tested SGI1 Protein 1e-89 45
merA AAN62178.1 mercuric (Hg(II)) reductase Not tested PAGI-2(C) Protein 2e-86 45
unnamed ABR13401.1 mercuric reductase Not tested PAGI-5 Protein 2e-88 44
merA YP_006098387.1 mercuric ion reductase Not tested Tn2411 Protein 2e-84 43
merA AET25397.1 MerA Not tested PAGI-2(C) Protein 1e-84 43
merA AFG30120.1 MerA Not tested PAGI-2 Protein 1e-84 43

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Btus_2787 YP_003590576.1 mercuric reductase BAC0651 Protein 1e-173 67
Btus_2787 YP_003590576.1 mercuric reductase BAC0224 Protein 2e-165 64
Btus_2787 YP_003590576.1 mercuric reductase BAC0652 Protein 5e-88 44
Btus_2787 YP_003590576.1 mercuric reductase BAC0650 Protein 1e-80 42
Btus_2787 YP_003590576.1 mercuric reductase BAC0653 Protein 1e-83 42