Gene Information

Name : Btus_1497 (Btus_1497)
Accession : YP_003589354.1
Strain : Kyrpidia tusciae DSM 2912
Genome accession: NC_014098
Putative virulence/resistance : Virulence
Product : flagellar protein export ATPase FliI
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.14
Position : 1510058 - 1511383 bp
Length : 1326 bp
Strand : +
Note : SMART: AAA ATPase; TIGRFAM: flagellar protein export ATPase FliI; ATPase, FliI/YscN family; KEGG: bld:BLi01844 flagellum-specific ATP synthase; PFAM: H+transporting two-sector ATPase alpha/beta subunit central region

DNA sequence :
GTGCAGGAAGGTTGGGACACCAACGCGTATTGCGAAACTCTTCGCCGCTCCCCCCTGTGGAGGGTTTCCGGGCGGGTCGC
CCAGGCGGTAGGCATGACGGTGGAATCGAACGGTCCCGTGGCGCGGGTGGGAGATCTCTGCTCGATTGAAACGGACGCCG
GACCGCGGTTTGCGGAGGTCGTGGGCTTCCGGAACCGCCGGCTCATTCTGATGCCCTTCTCCGACCTGGACCGCATCCAC
AATGGCGCCGTGGTGAAGGTGGTCTCCGAAGGCCTCCGGGTACCCACTGGGCCGGGCATTTTGGGGAGGGTTGTAGACGG
ACTCGGCCGGCCCATCGACGGGCTTGGGCCGGTGGCGGGGGAATTTCAAGATCCCATCGAGCAGCCTCCCCCGCCGCCCA
TGGCCCGAACCCCGGTGAACCAGCCCTTGTCCGTGGGGGTGAGGGCGATCGACGGGCTGCTCACGGTGGGACAAGGCCAG
AGGATCGGCATTTTCGCGGGGAGTGGAGTGGGGAAAAGCACCCTTTTAGGGATGATCGCCCGGTATACCGAAGCGGACGT
GACGGTGATTGGCCTGATTGGTGAGCGGGGAAGGGAAGTGCAGGATTTTATCGCCCGGGAGTTGGATGAAGAAGGGCGAA
AACGGGCGGTGGTGGTGGCTGCGACATCCGATCAGCCGCCCTTGATGCGGATCAAAGGGGCGCTCACCGCCACAGCCATG
GCGGAGCGGTTCCGGCGCCAGGGACGACGGGTTCTGTTGTTGATGGACTCTGTAACCCGTTTTGCCATGGCCTGCCGCGA
GGTGGGATTGGCCGCCGGCGAACCCCCCACCGCCCGGGGCTACACCCCTTCGGTCTTCGCCGCCCTGCCAAAACTGTTGG
AGCGCACGGGAACTGCTCGTACCGGCTCGATTACCGCCTTTTACACCGTCTTGGTGGAAGGGGACGATTTTAACGAACCC
ATCGCGGATGCCGTGCGGGGCATTCTGGACGGTCACATCGTATTATCCAGGAAAATCGCCCAACAGGGGCTCTATCCCGC
CATTGATGTGACGGCGAGTACCAGTCGCCTCATGAATCAAGTGGCGGAGACCGCTCACGTCCGGGCTGCGGCTCGGTTTA
AAGCCCTGTTGGCGGCGTACGAAGAAGCGGAAGATTTGATTCAAATCGGCGCCTATCGCGAGGGGACCCGACTGGAGGTA
GACACCGCGCTGCGCCTTAGACCCCGAATGTGGGCGTACCTCGCCCAGAATCGCGATGTGAGAAGTGACATGGGCGAGGC
GGTACGCCAGCTCATGGAGATGTTCAACGAGGAGGGCGGCGGATGA

Protein sequence :
MQEGWDTNAYCETLRRSPLWRVSGRVAQAVGMTVESNGPVARVGDLCSIETDAGPRFAEVVGFRNRRLILMPFSDLDRIH
NGAVVKVVSEGLRVPTGPGILGRVVDGLGRPIDGLGPVAGEFQDPIEQPPPPPMARTPVNQPLSVGVRAIDGLLTVGQGQ
RIGIFAGSGVGKSTLLGMIARYTEADVTVIGLIGERGREVQDFIARELDEEGRKRAVVVAATSDQPPLMRIKGALTATAM
AERFRRQGRRVLLLMDSVTRFAMACREVGLAAGEPPTARGYTPSVFAALPKLLERTGTARTGSITAFYTVLVEGDDFNEP
IADAVRGILDGHIVLSRKIAQQGLYPAIDVTASTSRLMNQVAETAHVRAAARFKALLAAYEEAEDLIQIGAYREGTRLEV
DTALRLRPRMWAYLAQNRDVRSDMGEAVRQLMEMFNEEGGG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 9e-80 53
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 4e-79 52
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 6e-79 52
lscN AAO18044.1 LscN Virulence TTSS locus Protein 2e-81 50
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-79 50
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 4e-80 50
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-79 50
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-79 50
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 6e-80 50
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 1e-79 50
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 4e-80 50
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 1e-74 48
escN AAC38383.1 EscN Virulence LEE Protein 4e-74 48
escN NP_290268.1 hypothetical protein Virulence LEE Protein 2e-74 48
ECs4568 NP_312595.1 EscN Virulence LEE Protein 2e-74 48
escN AAC31513.1 L0034 Virulence LEE Protein 1e-74 48
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 5e-70 48
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 4e-75 47
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 3e-75 47
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 4e-75 47
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 4e-75 47
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-75 47
escN YP_003223475.1 translocator EscN Virulence LEE Protein 1e-74 47
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 7e-75 47
escN CAI43874.1 EscN protein Virulence LEE Protein 1e-74 47
escN CAC81862.1 EscN protein Virulence LEE II Protein 7e-75 47
escN YP_003236088.1 translocator EscN Virulence LEE Protein 4e-75 47
escN AAK26715.1 EscN Virulence LEE Protein 2e-74 47
escN AAL57542.1 EscN Virulence LEE Protein 2e-74 47
unnamed AAL06369.1 EscN Virulence LEE Protein 2e-74 47
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 4e-69 47
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 6e-67 46
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 3e-73 46
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 3e-73 46
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 6e-72 45
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 3e-77 45
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 2e-71 45
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 1e-71 45
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 2e-70 45
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 2e-70 45
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 3e-68 45
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 4e-62 44
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 1e-66 43
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 1e-66 43
invC AAS66861.1 InvC Not tested SSR-2 Protein 3e-66 43
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 9e-63 43
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 9e-63 43
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 9e-63 43
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 9e-63 43
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 2e-64 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Btus_1497 YP_003589354.1 flagellar protein export ATPase FliI VFG0192 Protein 5e-82 54
Btus_1497 YP_003589354.1 flagellar protein export ATPase FliI VFG0390 Protein 3e-80 53
Btus_1497 YP_003589354.1 flagellar protein export ATPase FliI VFG2144 Protein 5e-86 51
Btus_1497 YP_003589354.1 flagellar protein export ATPase FliI VFG2499 Protein 2e-87 51
Btus_1497 YP_003589354.1 flagellar protein export ATPase FliI VFG0048 Protein 1e-85 50
Btus_1497 YP_003589354.1 flagellar protein export ATPase FliI VFG1254 Protein 1e-85 50
Btus_1497 YP_003589354.1 flagellar protein export ATPase FliI VFG2331 Protein 1e-85 49
Btus_1497 YP_003589354.1 flagellar protein export ATPase FliI VFG0812 Protein 7e-75 48
Btus_1497 YP_003589354.1 flagellar protein export ATPase FliI VFG0730 Protein 2e-74 48
Btus_1497 YP_003589354.1 flagellar protein export ATPase FliI VFG0515 Protein 2e-75 47
Btus_1497 YP_003589354.1 flagellar protein export ATPase FliI VFG1008 Protein 2e-61 44
Btus_1497 YP_003589354.1 flagellar protein export ATPase FliI VFG0555 Protein 4e-63 43
Btus_1497 YP_003589354.1 flagellar protein export ATPase FliI VFG2459 Protein 2e-65 42
Btus_1497 YP_003589354.1 flagellar protein export ATPase FliI VFG1778 Protein 2e-59 42