Gene Information

Name : Amico_0733 (Amico_0733)
Accession : YP_003553590.1
Strain : Aminobacterium colombiense DSM 12261
Genome accession: NC_014011
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : -
Position : 752034 - 753827 bp
Length : 1794 bp
Strand : +
Note : KEGG: dps:DP3116 pyruvate kinase; TIGRFAM: pyruvate kinase; PFAM: Pyruvate kinase barrel; PEP-utilising protein mobile region; HpcH/HpaI aldolase; Pyruvate kinase alpha/beta

DNA sequence :
TTGAGTGACCATATAGCTTATGTGATGGAAGGGGTCATAAAAATGAAGAAAGTGAAAATAGTCTGTACTATTGGCCCAGC
TTGTTCTACATATGAAATGCTTTGTTCCATGGCTGAAGCTGGCATGAATGTCGCCCGCTTTAATTTTAGTCACGGAGCAT
ACGAAGAACACAAAGCTCGTCTTGATCTTGTCAGAAAAGCTGAAAAAGAAATAGGCAAGCCCATAGCGACCCTTCTTGAT
ACGAAAGGACCGGAAATCCGTACCGGAACCTTAAAAGAAGGATTTATTGTGCTGATAAAAGACCAGAAGATTATTCTAAC
AACAAGGGACGTAGTGGGAGATTCTAAAGAGGTATATGTTAATTACCCCAGTCTTCCTTCAGAAGTAAATGTAGGGCAGG
ATATATACATTGATGATGGCACCCTGCATCTCAAAGTAACTAGTGTAGAGGGACTTGATGTTTTTTGTCAGGTCGTTGTA
GGCGGAGAACTGGGTGAAAGGAAGGGCGTCAATATTCCGGGAGCTTCTATCTCTCTTCCCGCACTTTCAGAAAAAGATCA
TAAGGATATTTGCTGGGGTCTTGAAAACGAAATGGATTATATAGCAGTATCCTTTGTCAAAAATCAAAAGGATATTATGG
AAGTACGGAAGATAATAGAAGATGCGGGCGGCGCCATGAAGATTATCGCAAAGATTGAAACTCGCCAGGCCGTTAATGCC
ATATTGGAAATTCTTGAAGTGGTGGATGGGGTTATGATTGCCAGGGGCGATCTAGGTGTAGAGATCCCAACAGAAGAAGT
TCCCCTTGTTCAAAAAGAGATTATTGACCTTTGCCGCTCAAAAGGCAAAGCTGTGATCGTTGCTACTCAGATGCTTGATT
CCATGATTAGGAATCCTCGTCCAACGAGAGCAGAGGCCAGTGATGTGGCGAATGCAGTACTTGATGGAACTGATGCTGTA
ATGCTTTCTGGTGAAACAGCAAAGGGGGCATACCCCCTGCGTGCCGTTGAAACTATGCAGCGTATTGTTGCTCGAGTAGA
GAAAGAACTTGAACTCTGGCAACATCCCCTTCATCGAAAACAATTAAGCACGGGCATCCCAGATGCTGTAAGCGGGGCAT
CCGTGGCAGTTGCTCGTGAAATGGGGGCGGCAGCTATTGTGTCATTAACAAGGAGCGGCAGCACTGCCCAGATGGTGAGC
AAACATCGTCCGCCATGTCTGATCATAGGAGCTACACCAGTTGTTCGTACTTGGAGAGAGCTTTCTCTCTATTGGGGTGT
AGAACCTCTCTTAGTTGAGAATATTAAAGATCAGGATGAAGCAGTGGCAAATGCCTTTACGGCAAGCTTCAAAAAAGGAC
TTCTCAAGGAAGGGGATCTCGTTGTAGTAACAGCAGGGGTCCCTATGGGCATTCCTGGAACGACGAATATGCTACAGGTT
CATACAGTAGGCAAAATTCTAGTGAAAGGCCTTTCCCTCTTGAAGAAAGAAGCATTTGGTGTTATTAAAACGGCTCGTAC
CGCAGAAGAGGCCCTTGGTAAAATGGAAGAGGGAGATATTCTAGTTGTTTCAGAAACAGACAGGGACTTCTTACCTGCTA
TGAGAAAGGCTGTTGCTATTATTACGGAACATGGCGGGCTAACAAGTCATGCAGCTATAGTTGCTTTAGAACTTGGAATT
CCTTGTGTTGTAAGCGCTAATAACGCTCTTTCTATTTTGCATGATGGCATGTCTGTTACTGTAGATGGCTTCCGTGGAGT
AATATACGCTGGGAGTGTTCATTTAAGAGCGTGA

Protein sequence :
MSDHIAYVMEGVIKMKKVKIVCTIGPACSTYEMLCSMAEAGMNVARFNFSHGAYEEHKARLDLVRKAEKEIGKPIATLLD
TKGPEIRTGTLKEGFIVLIKDQKIILTTRDVVGDSKEVYVNYPSLPSEVNVGQDIYIDDGTLHLKVTSVEGLDVFCQVVV
GGELGERKGVNIPGASISLPALSEKDHKDICWGLENEMDYIAVSFVKNQKDIMEVRKIIEDAGGAMKIIAKIETRQAVNA
ILEILEVVDGVMIARGDLGVEIPTEEVPLVQKEIIDLCRSKGKAVIVATQMLDSMIRNPRPTRAEASDVANAVLDGTDAV
MLSGETAKGAYPLRAVETMQRIVARVEKELELWQHPLHRKQLSTGIPDAVSGASVAVAREMGAAAIVSLTRSGSTAQMVS
KHRPPCLIIGATPVVRTWRELSLYWGVEPLLVENIKDQDEAVANAFTASFKKGLLKEGDLVVVTAGVPMGIPGTTNMLQV
HTVGKILVKGLSLLKKEAFGVIKTARTAEEALGKMEEGDILVVSETDRDFLPAMRKAVAIITEHGGLTSHAAIVALELGI
PCVVSANNALSILHDGMSVTVDGFRGVIYAGSVHLRA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 2e-87 47
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 1e-87 47
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 1e-87 47
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 6e-87 47
pykA NP_273151.1 pyruvate kinase Not tested IHT-A Protein 3e-61 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Amico_0733 YP_003553590.1 pyruvate kinase VFG0479 Protein 5e-88 47