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Name : EAM_1561 (EAM_1561) Accession : YP_003538643.1 Strain : Erwinia amylovora ATCC 49946 Genome accession: NC_013971 Putative virulence/resistance : Virulence Product : type III secretion system protein Function : - COG functional category : N : Cell motility COG ID : COG1157 EC number : - Position : 1681656 - 1682951 bp Length : 1296 bp Strand : + Note : - DNA sequence : ATGTTAACACAGATGTTAGTGCGTCTTGCTCATCCACTACGTATCCAGGGAAATGTGATAGAAGCATACCTTCCAGACAC AAAGATTGGAGAAATTTGTGAAATTCAAAAATCATTAGTTGAAAGTCAAGTCTTAGGGCTAGCCCAGGTAACCGGATTCA ATAAAGAACATACATTCTTAAGCCTCTTAACCGATAGCCAGGGGTTTTCACGTGAAAACGTATTGTTAGCAACAGGTAAA CCTTTGCGCGTTGATGTGCATCAAGGAATTTCAGGTGCAATAATTGATGCTACCGGGATAATCAGAGGGCGTCTGGATGA TGTGCCAGTTACTCCTTTAACGGGTACAAAGAGTATCATGGCCTGCGGGGCGGCGCGTGATTTTAGCTTGCGTAAAACGA TAACTGAACCATTGCTAACTGGGATTCGTGCCATTGATGGGTTACTAACATGTGGACAGGGCCAACGAGTCGGAATTTTT GCGACAGCAGGCTGTGGTAAAACATCGCTGATGAATATGCTCATCGAGCATTCACAAGCGGATATATATGTTATAGGCCT TATTGGTGAACGAGGACGAGAAGTTACTGAGTTTGTTGAATCTATGCGACATTCTTCGCGACGTTCACGAGTGATATTAG TTTATGCGACCTCAGATCGCTCTTCAGTAGAACGTACTAACGCCGCACAAATCGCATCAAGCATTGCCGAGTATTTCAGT GATATGGGCAAAAATGTACTACTTTTTATTGACTCAATTACCCGTTATGCACGTGCGTTACGTGATGTTGCCCTGAGTAT GGGAGAAATGCCAGCGCGCCGTGGCTATCCAGCATCGGTATTTGAGGCATTACCAAAGCTGCTCGAGCGGCCTGGTTGTT TCATCACCGGTACGATCACAGCATTCTATACTGTGCTAATTGAAAATGAAGAAGAGCCAGATATAATCGGTGATGAAGTA CGATCGATATTGGATGGGCATATATACCTGAGCCGTAAACTCGCCGCTAAAGGGCATTATCCGGCAATTAATGTTCTTAA CAGCCTCAGTCGAGTTTTTTCACAAGTTACAAATTGTCATCAGCAGCAAATGGCAAACAGTTTCCGAGATTTATTGCAGC GACAAAGTGAGATGCAATTATTTATCGATTTAGGGGAATATCGCCGGGGTGAAAACGAAAAAAACGATATTGCAATTGAT AAGAGCATACAAATGGAAGCATTTTTAAAACAAGACGTGACAGAGAAAATCGCAATATCGACCTGTCTGGATGAATTATA TGGATGCCTGGCATAG Protein sequence : MLTQMLVRLAHPLRIQGNVIEAYLPDTKIGEICEIQKSLVESQVLGLAQVTGFNKEHTFLSLLTDSQGFSRENVLLATGK PLRVDVHQGISGAIIDATGIIRGRLDDVPVTPLTGTKSIMACGAARDFSLRKTITEPLLTGIRAIDGLLTCGQGQRVGIF ATAGCGKTSLMNMLIEHSQADIYVIGLIGERGREVTEFVESMRHSSRRSRVILVYATSDRSSVERTNAAQIASSIAEYFS DMGKNVLLFIDSITRYARALRDVALSMGEMPARRGYPASVFEALPKLLERPGCFITGTITAFYTVLIENEEEPDIIGDEV RSILDGHIYLSRKLAAKGHYPAINVLNSLSRVFSQVTNCHQQQMANSFRDLLQRQSEMQLFIDLGEYRRGENEKNDIAID KSIQMEAFLKQDVTEKIAISTCLDELYGCLA |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| ysaN | AAS66842.1 | YsaN | Not tested | SSR-1 | Protein | 1e-125 | 65 |
| invC | AAS66861.1 | InvC | Not tested | SSR-2 | Protein | 2e-113 | 58 |
| invC | YP_217813.1 | ATP synthase SpaL | Virulence | SPI-1 | Protein | 2e-110 | 56 |
| spaI | NP_457288.1 | secretory apparatus ATP synthase (associated with virulence) | Virulence | SPI-1 | Protein | 2e-110 | 56 |
| spaI | NP_806497.1 | ATP synthase SpaL | Virulence | SPI-1 | Protein | 2e-110 | 56 |
| invC | NP_461815.1 | ATP synthase SpaL | Virulence | SPI-1 | Protein | 2e-110 | 56 |
| ECs3730 | NP_311757.1 | ATP synthase SpaL | Not tested | LIM | Protein | 1e-102 | 54 |
| YPO0267 | YP_002345348.1 | type III secretion system ATPase | Virulence | Not named | Protein | 4e-75 | 44 |
| XC_3006 | YP_244074.1 | type III secretion system ATPase | Virulence | Hrp PAI | Protein | 1e-78 | 44 |
| hrcN | NP_636610.1 | type III secretion system ATPase | Virulence | Hrp PAI | Protein | 1e-78 | 44 |
| hrcN | AAP34339.1 | HrcN | Virulence | Hrp PAI | Protein | 6e-77 | 44 |
| hrcN | NP_640767.1 | type III secretion system ATPase | Virulence | Hrp PAI | Protein | 3e-76 | 44 |
| hrcN | YP_362163.1 | type III secretion system ATPase | Virulence | Hrp PAI | Protein | 2e-75 | 44 |
| hrpB6 | AAB08461.1 | HrpB6 | Virulence | Hrp PAI | Protein | 1e-75 | 44 |
| hrcN | AAB06001.1 | HrcN | Virulence | Hrp PAI | Protein | 1e-66 | 44 |
| hrcN | AAG33879.1 | HrcN | Virulence | Hrp PAI | Protein | 3e-66 | 43 |
| ssaN | NP_460380.1 | type III secretion system ATPase | Virulence | SPI-2 | Protein | 2e-69 | 43 |
| ssaN | NP_456113.1 | putative type III secretion ATP synthase | Virulence | SPI-2 | Protein | 1e-69 | 43 |
| ssaN | NP_805086.1 | type III secretion system ATPase | Virulence | SPI-2 | Protein | 1e-69 | 43 |
| ssaN | CAA70537.1 | secretion system apparatus | Virulence | SPI-2 | Protein | 1e-69 | 43 |
| ssaN | YP_216423.1 | type III secretion system ATPase | Virulence | SPI-2 | Protein | 4e-70 | 43 |
| lscN | AAO18044.1 | LscN | Virulence | TTSS locus | Protein | 7e-77 | 43 |
| hrcN | NP_791227.1 | type III secretion cytoplasmic ATPase HrcN | Virulence | Hrp PAI | Protein | 3e-66 | 43 |
| hrcN | AAT96339.1 | HrcN | Virulence | S-PAI | Protein | 6e-70 | 43 |
| hrpB6 | BAB07852.1 | HrpB6 | Virulence | Hrp PAI | Protein | 4e-76 | 43 |
| hrcN | YP_198730.1 | type III secretion system ATPase | Virulence | Hrp PAI | Protein | 6e-76 | 43 |
| hrpB6 | AAF61281.1 | HrpB6 | Virulence | Hrp PAI | Protein | 4e-76 | 43 |
| hrcN | AAR25633.1 | type III secretion cytoplasmic ATPase HrcN | Not tested | Not named | Protein | 2e-60 | 42 |
| escN | AAC38383.1 | EscN | Virulence | LEE | Protein | 3e-67 | 42 |
| escN | AAC31513.1 | L0034 | Virulence | LEE | Protein | 2e-67 | 42 |
| escN | NP_290268.1 | hypothetical protein | Virulence | LEE | Protein | 2e-67 | 42 |
| unnamed | ACU09458.1 | type III secretion apparatus H+-transporting two-sector ATPase | Virulence | LEE | Protein | 2e-67 | 42 |
| ECs4568 | NP_312595.1 | EscN | Virulence | LEE | Protein | 2e-67 | 42 |
| escN | AAL57542.1 | EscN | Virulence | LEE | Protein | 2e-66 | 42 |
| escN | CAC81862.1 | EscN protein | Virulence | LEE II | Protein | 1e-66 | 42 |
| escN | YP_003223475.1 | translocator EscN | Virulence | LEE | Protein | 4e-66 | 42 |
| escN | YP_003232153.1 | T3SS structure protein EscN | Virulence | LEE | Protein | 2e-66 | 42 |
| unnamed | AAL06369.1 | EscN | Virulence | LEE | Protein | 1e-65 | 42 |
| escN | CAI43874.1 | EscN protein | Virulence | LEE | Protein | 3e-66 | 42 |
| escN | YP_003236088.1 | translocator EscN | Virulence | LEE | Protein | 1e-67 | 42 |
| escN | AAK26715.1 | EscN | Virulence | LEE | Protein | 2e-66 | 42 |
| hrcN | AAT96141.1 | HrcN | Virulence | T-PAI | Protein | 6e-68 | 42 |
| hrcN | AAT96195.1 | HrcN | Virulence | T-PAI | Protein | 5e-68 | 42 |
| hrcN | AAT96258.1 | HrcN | Virulence | S-PAI | Protein | 3e-69 | 42 |
| hrcN | AAT96299.1 | HrcN | Virulence | S-PAI | Protein | 2e-69 | 42 |
| hrcN | ABA47275.1 | HrcN | Virulence | S-PAI | Protein | 1e-72 | 42 |
| hrcN | ABQ88354.1 | HrcN | Virulence | Hrp PAI | Protein | 3e-65 | 42 |
| hrpJ4 | AAA81942.1 | HrpJ4 | Virulence | Hrp PAI | Protein | 3e-65 | 42 |
| escN | AFO66300.1 | putative LEE-encoded type III secretion system ATPase | Virulence | SESS LEE | Protein | 2e-65 | 42 |
| escN | AFO66415.1 | putative LEE-encoded type III secretion system ATPase | Virulence | SESS LEE | Protein | 2e-65 | 42 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| EAM_1561 | YP_003538643.1 | type III secretion system protein | VFG2459 | Protein | 3e-116 | 57 |
| EAM_1561 | YP_003538643.1 | type III secretion system protein | VFG1778 | Protein | 7e-107 | 57 |
| EAM_1561 | YP_003538643.1 | type III secretion system protein | VFG0555 | Protein | 1e-110 | 56 |
| EAM_1561 | YP_003538643.1 | type III secretion system protein | VFG1008 | Protein | 8e-103 | 54 |
| EAM_1561 | YP_003538643.1 | type III secretion system protein | VFG0048 | Protein | 3e-80 | 44 |
| EAM_1561 | YP_003538643.1 | type III secretion system protein | VFG0390 | Protein | 2e-75 | 44 |
| EAM_1561 | YP_003538643.1 | type III secretion system protein | VFG0192 | Protein | 1e-79 | 43 |
| EAM_1561 | YP_003538643.1 | type III secretion system protein | VFG2144 | Protein | 4e-74 | 43 |
| EAM_1561 | YP_003538643.1 | type III secretion system protein | VFG0515 | Protein | 6e-70 | 43 |
| EAM_1561 | YP_003538643.1 | type III secretion system protein | VFG0812 | Protein | 1e-67 | 42 |
| EAM_1561 | YP_003538643.1 | type III secretion system protein | VFG0730 | Protein | 2e-67 | 42 |
