Gene Information

Name : hrcN (EAMY_0532)
Accession : YP_003529890.1
Strain : Erwinia amylovora CFBP1430
Genome accession: NC_013961
Putative virulence/resistance : Virulence
Product : ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : -
Position : 606676 - 608034 bp
Length : 1359 bp
Strand : -
Note : ATP synthase subunit alpha (ATPase subunit alpha) (ATP synthase F1 sector subunit alpha), Type III secretion system ATPase, FliI/YscN, ATP synthase alpha/beta family, nucleotide-binding domain

DNA sequence :
ATGAGCGCGCTACAGCAGCGTCTGACGCAGTGGGCGCAGCAGCATCAGCGCCGCCTGGAGCGCTACGCGCCCGTTAGCCG
TTACGGCCGTGTGACGGGTATCAGCGGCATTCTGATCGAATGCATCCTGCCGGGCGCGCGCATCGGCGATTTATGCCGTA
TCCAACGCAGTGACGGCGGCAGCGTGCTGTCAGAGATCGTGGGCTTCAGCCCGGAGAAAATCCTGCTGTCGGCGCTGGGC
GCGCTGGACGGTATTTCACAGGGAGCCACTATCGTCCCGCTTTATCTGCCGCACAGTATTTGCGTCAGTGAGCAACTGCT
GGGCAGCGTGCTGGATGGTTTCGGCCGCGCGCTGGAGCCTGGCGGGCACAGCGCTTTCGCCGAACCGGGCACCCAGGTAC
GCGCCGTACCGGTGCTGAACGATGCGCCGCCCCCGACCGAGCGCCCGCGTATCACGACCCCGCTGCCCACCGGACTGCGG
GCGATTGACGGCCTGCTGACCATCGGTAACGGCCAGCGCGTGGGTATCTTTGCCGGAGCAGGCTGCGGCAAAACTACGCT
GCTGGCGGAGCTGGCGCGCAACACCCCGTGCGATGCCATCGTCTTTGGCCTGATCGGCGAGCGTGGTCGTGAACTGCGCG
AGTTTCTTGACCACGAGCTGGATGATGAATTACGCAGCCGCACGGTGCTGCTGTGCGCCACTTCGGACCGCAGCAGTATG
GAGCGCGCCCGCGCCGCATTTACCGCCACCGCCATCGCCGAGGCGTTTCGCGCCGAGGGCAAAAGCGTGCTGCTGATCGT
CGATTCACTCACCCGCTTTGCCCGCGCACAGCGTGAGATTGGCCTGGCGCTTGGCGAACCGCCCGGTCGTGGCGGGCTGC
CACCATCGGTTTACACCCTGCTGCCAAGACTGGTTGAACGGGCCGGACAAACCCAACAGGGGGCGATTACCGCCCTGTAT
TCAGTGCTGATCGAGCAGGATTCGATGAACGATCCGGTGGCCGATGAAGTGCGTTCCCTGATCGACGGTCACATCGTACT
AAGCCGCCGCCTGGCCGAACGCAACCACTATCCAGCCATCGATGTTTTAGCCAGCCTGAGCCGTACCATGAGCAACGTGG
TCGAACCCGGCCACATGATGCAGGCCGGGCGGCTGCGCAGCCTGATGGCAGCGTATCAGCAGGTTGAAATGCTGATCCGC
CTGGGTGAATACCAACCCGGAAATGACAGCCTGACCGATGCCGCCGTCAACGCCAATGACATTATCAACCGGTTCCTGCG
CCAGTCGATGCGCGCCCCCGATCCTTTTGAACAAACCCAGTATCAACTTGCGGAGGTCAGCGCCCATGCCCCTGATTGA

Protein sequence :
MSALQQRLTQWAQQHQRRLERYAPVSRYGRVTGISGILIECILPGARIGDLCRIQRSDGGSVLSEIVGFSPEKILLSALG
ALDGISQGATIVPLYLPHSICVSEQLLGSVLDGFGRALEPGGHSAFAEPGTQVRAVPVLNDAPPPTERPRITTPLPTGLR
AIDGLLTIGNGQRVGIFAGAGCGKTTLLAELARNTPCDAIVFGLIGERGRELREFLDHELDDELRSRTVLLCATSDRSSM
ERARAAFTATAIAEAFRAEGKSVLLIVDSLTRFARAQREIGLALGEPPGRGGLPPSVYTLLPRLVERAGQTQQGAITALY
SVLIEQDSMNDPVADEVRSLIDGHIVLSRRLAERNHYPAIDVLASLSRTMSNVVEPGHMMQAGRLRSLMAAYQQVEMLIR
LGEYQPGNDSLTDAAVNANDIINRFLRQSMRAPDPFEQTQYQLAEVSAHAPD

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 0.0 100
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 8e-128 72
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 1e-126 71
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 6e-126 71
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 2e-126 71
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 4e-108 69
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 8e-106 68
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 1e-117 67
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 8e-118 67
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 9e-116 66
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 3e-109 62
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 3e-109 62
lscN AAO18044.1 LscN Virulence TTSS locus Protein 3e-81 53
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-73 50
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 6e-74 50
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 4e-74 50
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 4e-74 50
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 4e-74 50
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-69 50
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 6e-70 50
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 2e-69 50
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 3e-69 50
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-69 50
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 8e-73 49
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 6e-73 49
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-69 49
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-69 49
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 2e-68 47
escN YP_003223475.1 translocator EscN Virulence LEE Protein 4e-61 46
escN AAC31513.1 L0034 Virulence LEE Protein 5e-61 46
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 5e-61 46
escN NP_290268.1 hypothetical protein Virulence LEE Protein 6e-61 46
escN CAI43874.1 EscN protein Virulence LEE Protein 3e-61 46
ECs4568 NP_312595.1 EscN Virulence LEE Protein 6e-61 46
escN YP_003236088.1 translocator EscN Virulence LEE Protein 2e-61 45
escN AAC38383.1 EscN Virulence LEE Protein 6e-61 45
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 5e-61 44
escN AAK26715.1 EscN Virulence LEE Protein 4e-61 44
escN AAL57542.1 EscN Virulence LEE Protein 4e-61 44
escN CAC81862.1 EscN protein Virulence LEE II Protein 3e-61 44
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 2e-64 44
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 2e-64 44
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 5e-56 44
unnamed AAL06369.1 EscN Virulence LEE Protein 5e-60 44
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 8e-55 44
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 8e-55 44
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 8e-55 44
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 8e-55 44
invC AAS66861.1 InvC Not tested SSR-2 Protein 5e-54 41
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 4e-53 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
hrcN YP_003529890.1 ATP synthase VFG0390 Protein 1e-78 54
hrcN YP_003529890.1 ATP synthase VFG0048 Protein 3e-82 53
hrcN YP_003529890.1 ATP synthase VFG0192 Protein 2e-82 53
hrcN YP_003529890.1 ATP synthase VFG0515 Protein 1e-69 50
hrcN YP_003529890.1 ATP synthase VFG2144 Protein 2e-75 47
hrcN YP_003529890.1 ATP synthase VFG0812 Protein 3e-61 46
hrcN YP_003529890.1 ATP synthase VFG1254 Protein 3e-62 46
hrcN YP_003529890.1 ATP synthase VFG0730 Protein 3e-61 45
hrcN YP_003529890.1 ATP synthase VFG1008 Protein 8e-54 44
hrcN YP_003529890.1 ATP synthase VFG0555 Protein 3e-55 44
hrcN YP_003529890.1 ATP synthase VFG1778 Protein 1e-55 44
hrcN YP_003529890.1 ATP synthase VFG2331 Protein 9e-62 42
hrcN YP_003529890.1 ATP synthase VFG2459 Protein 7e-57 42