Gene Information

Name : fliI3 (EAMY_2691)
Accession : YP_003532046.1
Strain : Erwinia amylovora CFBP1430
Genome accession: NC_013961
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : -
Position : 2771215 - 2772579 bp
Length : 1365 bp
Strand : -
Note : Type III secretion system ATPase, FliI/YscN family

DNA sequence :
ATGACCCGGCATTTGAAAAGCTGGCTGGACACTATCGAACGCACCGAAGCCAAAATGGGCGCAATCCCGGCGTTTCGCCG
TTATGGCCGCCTGACTCGTGCCACCGGGCTGGTGATGGAAGCCGTGGGGCTAAAGCTGCCCATTGGGGCAATCTGTGTCG
TAGAGCGCCAGACCCGTAACGGTATTATGCCGGTCGAATGTGAAGTGGTGGGTTTTAAAGACCAGCTGCTTTATCTGATG
CCGTTGGAACATGTGGATGGCGTGCTGCCGGGGACGCGCGTATACGCCCCGGATGGCGACAGCTACGAGGGTAAACAGCT
ACCGGTCGGGCCACAGCTGCTTGGCCGGGTGCTGGATGCGCAGGGACGCCCGCTGGACGGACGTCCGGCACCGTTGAAAA
CCAGCAGCGCCAGCCTGTTTACCCAGCCGATTAACCCGCTGCACCGCGACCCGATCAAAAGCGTGCTTGACGTTGGCGTC
CGCGCCATCAACGGCCTGCTGACCGTCGGACGCGGGCAGCGCATGGGCCTGTTTGCCGGTTCCGGGGTGGGTAAAAGCGT
GCTGCTGGGCATGATGGCGCGTTATACCGATGCAGATGTGATCGTCGTCGGGCTGATCGGTGAGCGTGGGCGCGAAGTCA
AAGATTTTATCGAGAACATTCTTGGGGCAGAGGGGATCGAACGCGCGGTGGTGATCGCTGCGCCAGCCGATGTTTCGCCT
GTTTTGCGCATGCAGGGCGCGGTCTACGCTACCCGTCTGGCGGAGGATTTCCGCGATCGCGGAATGAACGTCCTGCTGAT
CATGGACTCCCTGACGCGTTTTGCCATGGCGCAGCGTGAGGTGGCATTGGCGATCGGTGAGCCGCCGGCAACCAAAGGTT
ATCCGCCATCGGTGTTTGCGCGCCTGCCGGCGCTGGTAGAGCGTGCGGGCAACGGCGTTGCCGGGGGCGGTTCTGTCACC
GCTTTTTATACCGTTCTCACCGAAGGCGACGACCAGCAAGACCCGATCGCGGATTCGGCACGCGCCATTCTTGACGGGCA
TATTGTGCTGTCACGTCGTCTCGCCGAGTCCGGCCACTATCCGGCAATCGATATTGAAGCCTCGATCAGCCGTGCGATGA
CCGAGCTTATTCCGCACGCGCAGTACCGTAATGTGCAAAGCTTCAAGCAGCAACTCTCATCGTATCAGCGCAACAGCGAC
CTGGTCAGCGTGGGGGCTTATGTCAGCGGCAGCGATGCCCTGCTCGATCGCGCCATTGCGCGTTATCCGCAGATGGAAAG
CTATTTGCACCAGGCGGTCCATGAAAGCTGCGGCTATGCAGAAGCCGTTAGCGCGCTCTCTGCGCTGCTGCCTGAAGTGA
GCTGA

Protein sequence :
MTRHLKSWLDTIERTEAKMGAIPAFRRYGRLTRATGLVMEAVGLKLPIGAICVVERQTRNGIMPVECEVVGFKDQLLYLM
PLEHVDGVLPGTRVYAPDGDSYEGKQLPVGPQLLGRVLDAQGRPLDGRPAPLKTSSASLFTQPINPLHRDPIKSVLDVGV
RAINGLLTVGRGQRMGLFAGSGVGKSVLLGMMARYTDADVIVVGLIGERGREVKDFIENILGAEGIERAVVIAAPADVSP
VLRMQGAVYATRLAEDFRDRGMNVLLIMDSLTRFAMAQREVALAIGEPPATKGYPPSVFARLPALVERAGNGVAGGGSVT
AFYTVLTEGDDQQDPIADSARAILDGHIVLSRRLAESGHYPAIDIEASISRAMTELIPHAQYRNVQSFKQQLSSYQRNSD
LVSVGAYVSGSDALLDRAIARYPQMESYLHQAVHESCGYAEAVSALSALLPEVS

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-84 47
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 9e-85 47
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 7e-85 47
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 7e-85 47
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 5e-80 47
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 1e-68 47
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 5e-69 47
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 1e-68 47
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 7e-69 47
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-85 46
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 2e-85 46
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 1e-85 46
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 5e-82 46
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 5e-82 46
lscN AAO18044.1 LscN Virulence TTSS locus Protein 6e-79 45
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-73 44
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-73 44
unnamed AAL06369.1 EscN Virulence LEE Protein 1e-72 43
escN CAC81862.1 EscN protein Virulence LEE II Protein 8e-75 43
escN CAI43874.1 EscN protein Virulence LEE Protein 7e-75 43
escN NP_290268.1 hypothetical protein Virulence LEE Protein 3e-74 43
ECs4568 NP_312595.1 EscN Virulence LEE Protein 3e-74 43
escN AAC38383.1 EscN Virulence LEE Protein 4e-74 43
escN YP_003236088.1 translocator EscN Virulence LEE Protein 1e-74 43
escN AAC31513.1 L0034 Virulence LEE Protein 2e-74 43
escN YP_003223475.1 translocator EscN Virulence LEE Protein 9e-75 43
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 2e-74 43
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 9e-75 43
escN AAK26715.1 EscN Virulence LEE Protein 1e-74 43
escN AAL57542.1 EscN Virulence LEE Protein 1e-74 43
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 3e-67 42
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-69 41
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 2e-63 41
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 6e-73 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI3 YP_003532046.1 flagellum-specific ATP synthase VFG2331 Protein 2e-154 73
fliI3 YP_003532046.1 flagellum-specific ATP synthase VFG2499 Protein 7e-139 67
fliI3 YP_003532046.1 flagellum-specific ATP synthase VFG1254 Protein 1e-120 61
fliI3 YP_003532046.1 flagellum-specific ATP synthase VFG2144 Protein 1e-88 47
fliI3 YP_003532046.1 flagellum-specific ATP synthase VFG0515 Protein 3e-69 47
fliI3 YP_003532046.1 flagellum-specific ATP synthase VFG0192 Protein 1e-84 46
fliI3 YP_003532046.1 flagellum-specific ATP synthase VFG0048 Protein 2e-79 44
fliI3 YP_003532046.1 flagellum-specific ATP synthase VFG0390 Protein 1e-80 44
fliI3 YP_003532046.1 flagellum-specific ATP synthase VFG0812 Protein 1e-74 43
fliI3 YP_003532046.1 flagellum-specific ATP synthase VFG0730 Protein 2e-74 43