Gene Information

Name : Slit_0595 (Slit_0595)
Accession : YP_003523222.1
Strain : Sideroxydans lithotrophicus ES-1
Genome accession: NC_013959
Putative virulence/resistance : Virulence
Product : flagellar protein export ATPase FliI
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.14
Position : 604736 - 606232 bp
Length : 1497 bp
Strand : -
Note : SMART: AAA ATPase; TIGRFAM: flagellar protein export ATPase FliI; ATPase, FliI/YscN family; KEGG: neu:NE2086 flagellar ATP synthase; PFAM: H+transporting two-sector ATPase alpha/subunit beta central region

DNA sequence :
ATGATCGACAGCCCCGACATCGTTGCGCAAGACGCTCCGGCACAGGCAGTGCCGCAGGGAGCCGCTGTTGCAGATGTCCA
CACAGCAACGGCCAGCCGCAACCGCCACCATCAGCGCTGGCAGGAACTGCTGCACCTGAACCGCGACCTGGTAGCACAAA
GTAATCCCTTGCTGGTAAGCGGCCGCCTGACCAAAGTCACCGGACTGGTGATGGAAGCAGTCGGCTTGCGCATGGCCGTT
GGCAGCACCTGCTGGATCGAACTGCCGAATACCCGCATCGAAGCCGAAGTGGTCGGCTTCGCCGGAGACAGATTGTTCCT
GATGCCGGAGAACGACGTACAGGGACTGGTTCCCGGCGCGCGCGTGACTCCGGCCGAACCCATGCGCGCGCCGGTGGCGC
CAGGCCAATCGCAGTTGCCGCGCCGCCGCAGCTCGGATCTGGCCCGTCATCTGCCGGTCGGTGAACACCTGCTGGGACGC
GTGCTCGACGGTGCGGGACGCCCGCTCGATCAATTCGGCCCCTTGCTGGAAAGCAAGAGCGCGCCGCTGCAAAGCCGCCC
GATCAACCCGCTGGAAAGGGCCGCGATCAAGGAAGCGCTGGATGTCGGCGTGCGCGCCATCAACAGCCTGCTCACTGTCG
GACGCGGCCAGCGCATGGGCCTGTTCGCCGGCTCCGGTGTCGGCAAGAGCGTTTTGCTCGGCATGATGGCACGCTACACC
AGCGCCGACGTGATCGTGGTCGGACTGATCGGCGAACGCGGACGCGAAGTCAAGGAATTCATCACCGACATCCTCGGCCA
GGAAGGATTGAGACGCTCGGTCGTGGTCGCCGCTCCCGCCGACACCAGCCCGTTGATGAGATTGCAGGGCGCAGCCTACG
CCACCACCATCGCCGAATATTTCCGCGACCAGGGCAAGAACGTGCTGCTCATCATGGATTCGCTCACCCGCTACGCCATG
GCGCAACGCGAGATCGCACTGGCCATCGGCGAGCCGCCCGCGACCAAGGGCTATCCGCCCTCGGTATTCGCCAAACTGCC
GCAGCTGGTAGAGCGTGCCGGCAACGGCGCGGAAGGCGGCGGCTCGATCACCGCGTTCTACACCGTGCTGACCGAAGGCG
ACGACCAGCAGGACCCCATCGCCGACAGTGCGCGCGCGATCCTCGACGGCCACATCGTGCTGTCCCGCCGACTCGCGGAA
CAGGGGCACTACCCTGCCATCGACATCGAAGCCTCGATCAGCCGCGCCATGAACCATCTGGTCAGTCCGCAGCATTTCCT
GCAGGTGCAGCGCTTCAAGCATATGTACGCACACTACCAGCGCAATCATGACCTGATCAGTGTGGGCGCCTACGTCAAGG
GCAGCGACCCGCTGCTGGATCAGGCTATCCAACTCTATCCGCACATGGAACATTTCCTCAAGCAAGGCATGTACGAGTCG
GAGGTCTATACTGCCAGCATGGGCAAATTTTCCGATTTGTTCCCGAATGAAGGTTAA

Protein sequence :
MIDSPDIVAQDAPAQAVPQGAAVADVHTATASRNRHHQRWQELLHLNRDLVAQSNPLLVSGRLTKVTGLVMEAVGLRMAV
GSTCWIELPNTRIEAEVVGFAGDRLFLMPENDVQGLVPGARVTPAEPMRAPVAPGQSQLPRRRSSDLARHLPVGEHLLGR
VLDGAGRPLDQFGPLLESKSAPLQSRPINPLERAAIKEALDVGVRAINSLLTVGRGQRMGLFAGSGVGKSVLLGMMARYT
SADVIVVGLIGERGREVKEFITDILGQEGLRRSVVVAAPADTSPLMRLQGAAYATTIAEYFRDQGKNVLLIMDSLTRYAM
AQREIALAIGEPPATKGYPPSVFAKLPQLVERAGNGAEGGGSITAFYTVLTEGDDQQDPIADSARAILDGHIVLSRRLAE
QGHYPAIDIEASISRAMNHLVSPQHFLQVQRFKHMYAHYQRNHDLISVGAYVKGSDPLLDQAIQLYPHMEHFLKQGMYES
EVYTASMGKFSDLFPNEG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 2e-81 49
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 1e-71 47
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 1e-71 47
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 4e-71 47
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 6e-71 47
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-71 47
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 5e-79 46
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 3e-79 46
lscN AAO18044.1 LscN Virulence TTSS locus Protein 4e-80 45
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 9e-81 45
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 7e-81 45
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 5e-80 45
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 7e-81 45
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 9e-81 45
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 4e-63 45
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-77 44
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-77 44
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 6e-72 44
escN YP_003236088.1 translocator EscN Virulence LEE Protein 1e-73 44
unnamed AAL06369.1 EscN Virulence LEE Protein 3e-73 44
escN AAL57542.1 EscN Virulence LEE Protein 2e-73 44
escN AAC31513.1 L0034 Virulence LEE Protein 2e-73 44
escN NP_290268.1 hypothetical protein Virulence LEE Protein 2e-73 44
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 2e-73 44
ECs4568 NP_312595.1 EscN Virulence LEE Protein 2e-73 44
escN CAC81862.1 EscN protein Virulence LEE II Protein 1e-73 44
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 2e-73 44
escN AAC38383.1 EscN Virulence LEE Protein 7e-74 44
escN CAI43874.1 EscN protein Virulence LEE Protein 3e-74 44
escN YP_003223475.1 translocator EscN Virulence LEE Protein 4e-74 44
escN AAK26715.1 EscN Virulence LEE Protein 2e-73 44
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 8e-67 43
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 8e-67 43
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 5e-75 43
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 2e-66 42
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 6e-69 42
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 1e-68 42
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 9e-69 42
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 8e-67 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Slit_0595 YP_003523222.1 flagellar protein export ATPase FliI VFG2331 Protein 5e-139 68
Slit_0595 YP_003523222.1 flagellar protein export ATPase FliI VFG2499 Protein 4e-147 68
Slit_0595 YP_003523222.1 flagellar protein export ATPase FliI VFG1254 Protein 6e-129 62
Slit_0595 YP_003523222.1 flagellar protein export ATPase FliI VFG0515 Protein 3e-71 47
Slit_0595 YP_003523222.1 flagellar protein export ATPase FliI VFG0048 Protein 1e-85 46
Slit_0595 YP_003523222.1 flagellar protein export ATPase FliI VFG0390 Protein 1e-80 46
Slit_0595 YP_003523222.1 flagellar protein export ATPase FliI VFG2144 Protein 9e-84 46
Slit_0595 YP_003523222.1 flagellar protein export ATPase FliI VFG0192 Protein 4e-85 45
Slit_0595 YP_003523222.1 flagellar protein export ATPase FliI VFG0730 Protein 4e-74 44
Slit_0595 YP_003523222.1 flagellar protein export ATPase FliI VFG0812 Protein 9e-74 44