Gene Information

Name : topB (PANA_2102)
Accession : YP_003520397.1
Strain : Pantoea ananatis LMG 20103
Genome accession: NC_013956
Putative virulence/resistance : Unknown
Product : TopB
Function : -
COG functional category : L : Replication, recombination and repair
COG ID : COG0550
EC number : -
Position : 2348742 - 2350658 bp
Length : 1917 bp
Strand : -
Note : DNA topoisomerase III; Similar to Pantoea sp. At-9b, DNA topoisomerase III (NCBI: ZP_05729421.1); COG: DNA replication, recombination and repair; Subcellular localization as predicted by Psort 2.0: Cytoplasmic

DNA sequence :
ATGCGTTTGTTTATTGCAGAGAAACCCAGCCTTGGACGGGCCATTGCCGATGTACTGCCTAAACCGCACCGGCGTGGTGA
GGGCTTTATCGCCTGCGGCAATGACCAGATTGTCACCTGGTGCGTGGGGCATTTACTGGAGCAGGCGCAGCCTGATGTGT
ACGACAGCCGCTATGCGCGCTGGTCGCTGGTCGATCTGCCGATTATTCCTGAAAAATGGCGTTTGCAGCCGCGTCCTTCG
GTGGCAAAACAACTCAAAGTGGTGGAAGGCTTACTGGCCCAGGCCGATGAAGTGGTGCACGCCGGCGACCCGGACAGGGA
AGGCCAGCTGCTTGTGGATGAAGTGCTGGATTACCTTCAGCTTCCCGCTGAGAAACGCAGTAAAGTGCAGCGCTGTCTGA
TTAACGATCTTAACCCACAGGCTGTGGAGCGGGCTATCAACCGGCTGCGTGAGAATCGCGAATTTATTCCGCTCTGCGTG
TCCGCGCTGGCGCGAGCACGTGCCGACTGGCTTTACGGTATTAATATGACCCGCGCCTGGACGCTGCTGGGCCGGAATGC
GGGCTATGATGGCGTGTTGTCCGTTGGACGCGTACAAACGCCGGTGTTGGGGCTGGTGGTGCGACGGGATGAAGAGATCG
CGAATTTCGTCCCGAAGGATTACTTTGAAGTGAAAGCGCACATCGTCACGCCGGATGAGAGCCGGTTTACCGCGAACTGG
ATCCCCAGTGAAGCCTGTGAACCCTGGCAGGATGAAGAAGGTCGCCTGCTGAAACGCTCCCTGGCGGAACATGTGGTGGC
GCGCATCAGCGGTCAGCCCGCTATCGTTACCGCCTACAGTGACAAGCGAGAAAACGACATTGCACCGTTGCCGTTTTCAC
TCTCCAGCCTGCAAATCGAGGCGGCAAAGCGCTTTGGTTTGAGTGCACAGGCGGTGCTGGACATCTGCCAGCGGCTATAT
GAAACCCACAAGCTGATCACCTATCCACGTTCTGATAGCCGCTACTTACCCGATGAGCATTTTGCCGGCCGTCATGCGGT
GCTCAATGCGATCCAGTCCCATCAATCCGGTCTTATTCCACCAGCAGACTTTGATGCAGATCGAAAAAATCGCTGTTGGG
ATGACAAAAAAGTGGATGCGCACCACGCCATTATTCCTACCGCGCGCGCCAGCGCAGTGAAGCTTAATGAAAACGAACAG
CAGATTTATGGACTGGTTGCGCGTCAGTATTTGATGCAATTTTGTCCGGATGCCGTGTTCCGCAAATGCACTATCGATCT
GGATATCGCGGGCGGTAAATTTGTGGCTAAAGCGCGCTTTCTGGCGGAGGCGGGCTGGCGTGCGCTGTTGGGTGGCAAAG
AGCGCGATGAAGAAAACGATGGCATGCCGCTGCCTGTTGTAGCGAAAGACGATGAGCTGCTGTGCGAGCGGGGTGAGGTG
CTGGCGAAACAGACACAACCGCCACGCCACTTTACCGACGCGACCTTGCTTTCGGCCATGACCGGCATTGCGCGCTTTGT
GCAGGATAAAGAATTAAAGAAAGTGCTGCGTGCGACCGATGGTTTAGGAACGGAAGCCACGCGCGCAGGCATTATTGAAT
TACTGTTCCGTCGTTCGTTTTTGCATAAGAAAGGGCGCTATATTCATTCTACTGATGCAGGCAAAGCGCTGATCCACTCG
CTACCGGATCTCGCCGCCAGGCCGGACATGACGGCGCAATGGGAATCAACCCTGACGCGTATCAGTGAAAAGGCCTGTCG
CTATGATGAATTTATGCAACCCCTGGTGCAGACGCTGAGCGGGCTTATTCAGCAGGCGCGACAGCAGCCCTCGGGACAGG
CGTTTCGTGGTTTACCGTCCACCGGCCAAAAACGTCCACCGCGTAAAGCAGGGCGTAAAACGAAGGAAACTGAATAA

Protein sequence :
MRLFIAEKPSLGRAIADVLPKPHRRGEGFIACGNDQIVTWCVGHLLEQAQPDVYDSRYARWSLVDLPIIPEKWRLQPRPS
VAKQLKVVEGLLAQADEVVHAGDPDREGQLLVDEVLDYLQLPAEKRSKVQRCLINDLNPQAVERAINRLRENREFIPLCV
SALARARADWLYGINMTRAWTLLGRNAGYDGVLSVGRVQTPVLGLVVRRDEEIANFVPKDYFEVKAHIVTPDESRFTANW
IPSEACEPWQDEEGRLLKRSLAEHVVARISGQPAIVTAYSDKRENDIAPLPFSLSSLQIEAAKRFGLSAQAVLDICQRLY
ETHKLITYPRSDSRYLPDEHFAGRHAVLNAIQSHQSGLIPPADFDADRKNRCWDDKKVDAHHAIIPTARASAVKLNENEQ
QIYGLVARQYLMQFCPDAVFRKCTIDLDIAGGKFVAKARFLAEAGWRALLGGKERDEENDGMPLPVVAKDDELLCERGEV
LAKQTQPPRHFTDATLLSAMTGIARFVQDKELKKVLRATDGLGTEATRAGIIELLFRRSFLHKKGRYIHSTDAGKALIHS
LPDLAARPDMTAQWESTLTRISEKACRYDEFMQPLVQTLSGLIQQARQQPSGQAFRGLPSTGQKRPPRKAGRKTKETE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
topB1 YP_005176228.1 DNA topoisomerase III Not tested ICEPmu1 Protein 2e-109 42
topB NP_458624.1 topoisomerase B Not tested SPI-7 Protein 2e-105 41
topB NP_807836.1 DNA topoisomerase III Not tested SPI-7 Protein 2e-105 41
topB AAF14809.1 topoisomerase B Not tested SPI-7 Protein 1e-105 41
topB AAN62317.1 putative DNA topoisomerase III Not tested PAGI-3(SG) Protein 1e-104 41