Gene Information

Name : pyk (HMPREF0868_0757)
Accession : YP_003475074.1
Strain : Clostridiales genomosp. UPII9-5
Genome accession: NC_013895
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 1034455 - 1036194 bp
Length : 1740 bp
Strand : -
Note : identified by match to protein family HMM PF00224; match to protein family HMM PF00391; match to protein family HMM PF02887; match to protein family HMM TIGR01064

DNA sequence :
ATGAGAAAAACAAAAATTATTTGTACATTGGGTCCGGCTACTGATGACGAAAATGTCTTGCGCGAATTGGTTCGCAGCGG
CATGAATTTGGCACGGTTTAATTTTTCTCACGGCACCCATGAAGAACATTTTAAACGTCTACAACTTTTGCGCCGTATTG
TTGCTGAAGAAGGTGCGACAGTTGCAACTTTGCTTGATACCAAAGGGCCGGAGATTCGTACCGGACAGTTCCCAGAAGGG
AAAGTTGAGCTTAAAATGGGCGAAAAGATAGTCATCAGACATGACGACGTGCCTGGAAATGCCCATGAGTTTTCCGTTAG
CTACAAAGACATGCATAAAGATGTGAAAGTCGGCTCACCGATCCTCATTGATGACGGCAGTGTTGAGTTGCTAGTTGACA
GCATTTCTCCGGAAGGTGACATCAGCTGTACCATCCAAAATGGCGGCTTTGTCAGCGATTATAAAAGCATCAATCTGCCC
GATTCTTTTGTGAATTTACCGGCACTGACTGAGCGTGATGTGAGTGATTTGAAATTTGCGGTTGATAACGACATGGATTT
TATCGCTGCTTCGTTCGTACGTCGCCCCTCTGATGTTGCCGAAATTCGCCAAACTTTGGAAAGATTCGGCAATCGTGATA
TCAGAATTATTGCCAAAATTGAAAATCGGGAAGGCGTAGATAAATTTGAGGAGATCTTACATGTATCTGACGCAATTATG
GTTGCTAGAGGCGATTTAGGTGTAGAAATACCTGCTTATGAAGTACCGCGTGTACAAAAGAACATGATCCGTCTTTGCTA
TAGATTTGGGATCCCCGCCATTACCGCAACGCAGATGCTCGACTCGATGATCCGCAATCCGCGTCCGACTCGTGCTGAAG
TCTCTGATGTAGCCAATGCTATTATGGACGGAACATCCGCAATCATGCTTTCCGGTGAAACCGCTTCAGGTAAATATCCG
GTTGAAGCTCTGCAGATGATGAGCAGAATTGCTGAAGAAACCGAACGTAACTACGATTATTGGGATTTCTTCCGTCACGC
GGGTGAGAAAGTACGCCCGACTGTAGGCAATGCGATCAGCCATTCATGCTGCACTACAGCTATGGACTTACAAGCTAAGG
CCATTGTTGCTATGTCCATTTCCGGCCGTACGGCGCGCCTCATTTCGCGCTTCAGACCTGGATGCCCGATTATTGCAACG
GCAGCTTCCGAGAAAGTCAGCCGTCAGTTACAGCTCAGCTGGGGTGTTATTCCTTATCACGTAAAACCGGTTAACACAAC
TGATGCCATATTCCAAAAAGGTGTGGACATAGCTGTTAATTCAGGCTTAGTAAAAACGGGTGATGTTGTAGTCATTACTT
GCGGAACACCGGTTGGTATGAGTGGAACTACTAACACCTTGAAAGTTCAAAACATCGGCAATGTTCTTTGCCAAGGCAAG
GGAATCGGTAAGGGCATAATTTCTAAAGAAGTGTTGAATACTTCGGAAAAAGATTTTAGTATGTCTAATATACCGAGGGA
CAAATATATTGTCGTAGCTAAAAATACCAATAATGATCTCATGCCGTTGTTGCGTCAAGCTGCCGGTATCGTGGTTGAAA
ATCCTGATCCTAACGGTCATGCAATCACCGTTGCTGAAGCTTTAAACATCCCAGTAATCTATGATTGCTCGAATGCAACC
AGTATTTTACGCAGTGGTCTGATTGTCAGCATGAATGTTAAATCAGGACTAATTGGATGA

Protein sequence :
MRKTKIICTLGPATDDENVLRELVRSGMNLARFNFSHGTHEEHFKRLQLLRRIVAEEGATVATLLDTKGPEIRTGQFPEG
KVELKMGEKIVIRHDDVPGNAHEFSVSYKDMHKDVKVGSPILIDDGSVELLVDSISPEGDISCTIQNGGFVSDYKSINLP
DSFVNLPALTERDVSDLKFAVDNDMDFIAASFVRRPSDVAEIRQTLERFGNRDIRIIAKIENREGVDKFEEILHVSDAIM
VARGDLGVEIPAYEVPRVQKNMIRLCYRFGIPAITATQMLDSMIRNPRPTRAEVSDVANAIMDGTSAIMLSGETASGKYP
VEALQMMSRIAEETERNYDYWDFFRHAGEKVRPTVGNAISHSCCTTAMDLQAKAIVAMSISGRTARLISRFRPGCPIIAT
AASEKVSRQLQLSWGVIPYHVKPVNTTDAIFQKGVDIAVNSGLVKTGDVVVITCGTPVGMSGTTNTLKVQNIGNVLCQGK
GIGKGIISKEVLNTSEKDFSMSNIPRDKYIVVAKNTNNDLMPLLRQAAGIVVENPDPNGHAITVAEALNIPVIYDCSNAT
SILRSGLIVSMNVKSGLIG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 7e-93 48
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 2e-93 47
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 1e-93 47
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 1e-93 47

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pyk YP_003475074.1 pyruvate kinase VFG0479 Protein 4e-94 47