Gene Information

Name : pykF (smi_1005)
Accession : YP_003446117.1
Strain : Streptococcus mitis B6
Genome accession: NC_013853
Putative virulence/resistance : Virulence
Product : pyruvate kinase I; fructose-stimulated
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 977931 - 979436 bp
Length : 1506 bp
Strand : +
Note : -

DNA sequence :
ATGAATAAACGTGTAAAAATCGTTGCAACTTTGGGTCCTGCGGTAGAAATCCGTGGTGGTAAAAAATTTGGTGATGACGG
ATACTGGGGTGAAAAACTTGATGTTGAAGCTTCAGCTAAAAACATTGCTAAATTGATTGAAGCTGGTGCTAACACATTCC
GTTTTAACTTCTCACACGGTGACCACCAAGAACAAGGTGAGCGTATGGCAACTGTTAAACTTGCTGAAAAACTTGCAGGT
AAAAAAGTTGGTTTCCTTCTTGATACAAAAGGACCAGAAATCCGTACAGAATTGTTCGAAGGTGAAGCTAAAGAGTATTC
ATACAAAACTGGTGAAAAAATCCGTGTTGCAACTAAACAAGGAATCAAATCAACTCGTGAAGTGATTGCATTGAACGTTG
CTGGTGCTCTTGATATCTATGATGATGTTGAAGTTGGTCGTCAAGTTTTGGTTGACGATGGTAAACTTGGTCTTCGTGTG
GTTGCTAAAGATGACGCAACTCGTGAATTCGAAGTTGAAGTTGAAAACGATGGTGTAATTGCTAAACAAAAAGGTGTTAA
CATTCCTAACACTAAAATTCCTTTCCCAGCTCTTGCTGAACGCGATAACGATGATATCCGCTTTGGTCTTGAACAAGGTA
TTAACTTTATCGCGATTTCATTCGTACGTACTGCTAAAGACGTTAACGAAGTTCGTGCAATCTGTGAAGAAACTGGAAAC
GGACACGTTCAATTGTTCGCTAAAATTGAAAACCAACAAGGTATCGATAACTTGGATGAAATCATCGAAGCTGCTGATGG
TATCATGATCGCTCGTGGTGACATGGGTATTGAAGTACCATTCGAAATGGTTCCAGTTTACCAAAAAATGATTATCACTA
AAGTCAATGCCGCTGGTAAAGTTGTTATCACTGCAACAAACATGCTTGAAACAATGACTGAAAAACCACGTGCAACTCGT
TCAGAAGTATCAGACGTATTTAACGCTGTTATCGACGGAACTGACGCAACAATGCTTTCAGGTGAGTCTGCAAACGGTAA
ATACCCACTTGAGTCAGTAACTACAATGGCTACAATCGACAAGAACGCTCAAACTCTTCTTAACGAATACGGACGTTTGA
ACTCAGATTCATTTGAACGTAACTCTAAGACAGAAGTAATGGCTTCAGCTGTTAAAGATGCTACTAACTCAATGGATATC
AAATTGGTTGTAACTCTTACTAAGACAGGTCACACTGCACGTTTGATTTCTAAATACCGTCCAAATGCTGACATCTTGGC
ATTGACATTCGACGAATTGACAGAACGTGGATTGATGTTGAACTGGGGTGTTATCCCAATGTTGACAGATGCTTCATCAT
CAACTGACGATATGTTCGAAATCGCTGAACGTAAAGCAGTTGAAGCAGGACTTGTACAATCTGGTGACGATATCGTTATC
GTAGCTGGTGTGCCAGTTGGAGAAGCTGTTCGTACAAACACAATGCGTATCCGCACAGTACGCTAA

Protein sequence :
MNKRVKIVATLGPAVEIRGGKKFGDDGYWGEKLDVEASAKNIAKLIEAGANTFRFNFSHGDHQEQGERMATVKLAEKLAG
KKVGFLLDTKGPEIRTELFEGEAKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
VAKDDATREFEVEVENDGVIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIAISFVRTAKDVNEVRAICEETGN
GHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMGIEVPFEMVPVYQKMIITKVNAAGKVVITATNMLETMTEKPRATR
SEVSDVFNAVIDGTDATMLSGESANGKYPLESVTTMATIDKNAQTLLNEYGRLNSDSFERNSKTEVMASAVKDATNSMDI
KLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTDASSSTDDMFEIAERKAVEAGLVQSGDDIVI
VAGVPVGEAVRTNTMRIRTVR

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 2e-80 45
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 6e-81 45
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 6e-81 45
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 4e-81 45

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pykF YP_003446117.1 pyruvate kinase I; fructose-stimulated VFG0479 Protein 3e-81 45